scholarly journals The Increased Formation of Alkali-Labile Sites by a Spin-Trapping Agent .ALPHA.-Phenyl-N-tert-Butylnitrone in .GAMMA.-Irradiated Frozen Aqueous Solution of DNA.

1997 ◽  
Vol 38 (3) ◽  
pp. 153-155 ◽  
Author(s):  
MIKINORI KUWABARA ◽  
HIDEKI OHSHIMA ◽  
YOSHIHARU IIDA ◽  
OSAMU INANAMI
Keyword(s):  
Aqueous Solution ◽  
Spin Trapping ◽  
Gamma Irradiated ◽  
Trapping Agent

The spin trapping agent PBN stimulates H2O2-induced Erk and Src kinase activity in human neuroblastoma cells

Neuroreport ◽  
2002 ◽  
Vol 13 (8) ◽  
pp. 1057-1061 ◽  
Author(s):  
Pelin Kelicen ◽  
Ippolita Cantuti-Castelvetri ◽  
Can Pekiner ◽  
K. Eric Paulson
Keyword(s):  
Kinase Activity ◽  
Neuroblastoma Cells ◽  
Src Kinase ◽  
Spin Trapping ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Trapping Agent

Paraquat-induced lung injury: prevention by N-tert-butyl-alpha-phenylnitrone, a free-radical spin-trapping agent

1992 ◽  
Vol 262 (2) ◽  
pp. L147-L152
Author(s):  
T. Sata ◽  
E. Kubota ◽  
H. P. Misra ◽  
M. Mojarad ◽  
H. Pakbaz ◽  
...  
Keyword(s):  
Free Radical ◽  
Lung Injury ◽  
Guinea Pig ◽  
Weight Ratio ◽  
Spin Trapping ◽  
Lung Weight ◽  
Paramagnetic Resonance ◽  
Arterial Perfusion ◽  
Tert Butyl ◽  
Trapping Agent

The herbicide paraquat causes lung injury that is believed to be oxygen-radical mediated. To further characterize this injury and explore new methods of preventing it, we used the spin-trapping agent N-tert-butyl-alpha-phenylnitrone (PBN) to identify the paraquat radical in lung tissue and to reduce the injury resulting from the subsequent generation of reactive oxygen species. The formation of a paraquat free radical by guinea pig lung was detected under anaerobic conditions by electron paramagnetic resonance spectrometry. Infused (25, 50, or 100 mg/kg) into guinea pig lungs (perfused at constant flow with Krebs solution containing 4% bovine serum albumin and ventilated with 95% O2-5% CO2), paraquat produced dose-dependent increases in peak airway pressure (Paw), mean pulmonary arterial perfusion pressure (Ppa), and wet-to-dry (W/D) lung weight ratio. At 100 mg/kg, paraquat increased Paw by 589.6 +/- 59.8% (mean +/- SE, n = 8) and W/D ratio from 5.33 +/- 0.07 to 6.29 +/- 0.11 (P less than 0.001). Pulmonary vascular leak index increased from 0.40 +/- 0.09 to 1.96 +/- 0.45 (P less than 0.02), without changes in pulmonary microvascular pressure. Perfusate concentrations of thromboxane B2 and 6-ketoprostaglandin F1 alpha increased, but indomethacin did not reduce the injury. PBN (2.3 mM) markedly attenuated all evidence of lung injury, which was also reduced by catalase, mannitol, ethanol, and vitamin E.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Pentamethylnitrosobenzene as a Spin-trapping Agent

1977 ◽  
Vol 50 (12) ◽  
pp. 3124-3126 ◽  
Author(s):  
Takahisa Doba ◽  
Tsuneki Ichikawa ◽  
Hiroshi Yoshida
Keyword(s):  
Spin Trapping ◽  
Trapping Agent
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