scholarly journals Incorporation of tritiated water (HTO) and organically bound tritium (OBT) into phospholipids and gangliosides of rat brain.

1985 ◽  
Vol 26 (4) ◽  
pp. 385-394
Author(s):  
MARIA KOWALSKA
1984 ◽  
Vol 19 (1) ◽  
pp. 101-110
Author(s):  
Thomas G. Dunstall

Abstract Chlorella pyrenoidosa, grown in batch culture under chronic exposure to tritiated water, was used to model behavior of tritium at the primary producer level of an aquatic food chain. The ratio of organically-bound tritium to tritium in the medium increased during initial growth, eventually reaching an asymptotic value of 0.59 after seven cell doublings. Loss of previously formed organically-bound tritium from cells transferred to tritium-free media averaged less than 5% for exponential growth phase cultures after three cell doublings. Over a comparable time period, organically-bound tritium lost from senescent cells averaged 13% which was attributed to increased degradative metabolism in stationary growth phase cultures. The concentration of tritium in organically-bound form may exceed environmental concentrations of tritium in water under dynamic conditions in which a pulse of tritiated water to the environment is dissipated over time.


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