scholarly journals Effect of low pH treatment and agents causing mitotic delay on intracellular pH of sea urchin eggs.

1981 ◽  
Vol 22 (1) ◽  
pp. 160-164
Author(s):  
H. KIMURA ◽  
T. SHIROYA ◽  
T. AOYAMA ◽  
A. SHIMA
Nature ◽  
1976 ◽  
Vol 262 (5570) ◽  
pp. 661-664 ◽  
Author(s):  
James D. Johnson ◽  
David Epel ◽  
Miles Paul

1997 ◽  
Vol 22 (4) ◽  
pp. 387-398 ◽  
Author(s):  
Miyako S. Hamaguchi ◽  
Kenji Watanabe ◽  
Yukihisa Hamaguchi

Author(s):  
Isabelle Galgani ◽  
Danielle Pesando ◽  
Jacqueline Porthe-Nibelle ◽  
Barthélémy Fossat ◽  
Jean-Pierre Girard

1983 ◽  
Vol 100 (1) ◽  
pp. 29-38 ◽  
Author(s):  
Patrick Payan ◽  
Jean-Pierre Girard ◽  
Brigitte Ciapa

1965 ◽  
Vol 26 (4) ◽  
pp. 534 ◽  
Author(s):  
Bhashini Rao ◽  
R. T. Hinegardner

1981 ◽  
Vol 89 (2) ◽  
pp. 284-291 ◽  
Author(s):  
C H Johnson ◽  
D Epel

Intracellular pH (pH1) of sea urchin eggs and embryos was determined using DMO (5,5-dimethyl-2,4-oxazolidinedione). By this method, the pH1 of Lytechinus pictus eggs increased after fertilization from 6.86 to 7.27, and this higher pHi was maintained thereafter, as has been previously observed with pH microelectrodes. The same general result was obtained with the eggs of Strongylocentrotus purpuratus, in contrast to previous estimates of the pH of egg homogenates from this species, which had indicated a rise and then fall of pHi after fertilization. pHi did not significantly change during early cell divisions. Studies of treatments that alter pHi confirmed that ammonia alkalizes and acetate acidifies the cells. The regulation of pHi by embryos in the acidic seawater is impaired if sodium is absent, whereas unfertilized eggs can regulate pHi in acidic, sodium-free seawater.


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