scholarly journals Assessing species and stage specific effects of preservation on fish oocytes over different temporal scales

2015 ◽  
Vol 16 (3) ◽  
pp. 533 ◽  
Author(s):  
M. RAKKA ◽  
K. GANIAS
Keyword(s):  
Developmental Stage ◽  
Elemental Composition ◽  
Oocyte Size ◽  
Temporal Scales ◽  
European Anchovy ◽  
Specific Effects ◽  
Fish Oocytes

This study assessed the effect of 10% neutral buffered formalin and of three ethanol solutions of different concentration on Mediterranean sardine and European anchovy oocytes over several temporal scales (days, weeks, months). The two species exhibit differences both in the elemental composition and the shape of their oocytes which further allowed an appraisal of oocyte shrinkage dynamics in relation to oocyte shape, developmental stage and composition. We showed that the effect of the preservative on oocyte size is stage specific while different preservation periods of ovarian material might lead to discrepancies among studies.

Resource matching across habitats is limited by competition at patch scales in an estuarine-opportunist fish

2005 ◽  
Vol 62 (4) ◽  
pp. 913-924 ◽  
Author(s):  
Karl M Polivka
Keyword(s):  
Interspecific Competition ◽  
Resource Availability ◽  
Large Scale ◽  
Substantial Reduction ◽  
Fish Growth ◽  
Matching Theory ◽  
Temporal Scales ◽  
Specific Effects ◽  
Resource Matching ◽  
Number Of Individuals

I used field observations, assays, and experiments with the euryhaline cottid Cottus aleuticus to evaluate the extent to which average resource availability drives the large-scale distribution of these fish among upstream and estuarine habitats and how interspecific competition from a congener affects its performance in the estuary. Population densities of C. aleuticus were only consistent with resource densities across years during two of five study years, indicating a lack of resource matching at large temporal scales. On shorter temporal scales, fish growth rates that were two to three times higher in the estuary compared with the stream were inconsistent with the predictions of resource matching theory. A manipulation of C. aleuticus density showed that the estuary could support at least twice the number of individuals that occurred there; thus, the profitable estuary is underutilized. Interspecific competition with Cottus asper was partially responsible for this underutilization as indicated by a substantial reduction in growth and condition among C. aleuticus individuals in experimental manipulations that compared intra- and inter-specific effects. Observed spatial overlap between these two cottids combined with the results of the competition experiment suggests that C. aleuticus is more strongly limited in its ability to use estuarine habitats opportunistically by interspecific competition than by intraspecific competition.

scholarly journals Species- and developmental stage-specific effects of allelopathy and competition of invasive Impatiens glandulifera on co-occurring plants

PLoS ONE ◽  
2018 ◽  
Vol 13 (11) ◽  
pp. e0205843 ◽  
Author(s):  
Judith Bieberich ◽  
Marianne Lauerer ◽  
Maria Drachsler ◽  
Julian Heinrichs ◽  
Stefanie Müller ◽  
...  
Keyword(s):  
Developmental Stage ◽  
Impatiens Glandulifera ◽  
Specific Effects

scholarly journals Developmental Stage-Specific Effects of Parenting on Adolescents’ Emotion Regulation: A Longitudinal Study From Infancy to Late Adolescence

2021 ◽  
Vol 12 ◽  
Author(s):  
Jaakko Tammilehto ◽  
Raija-Leena Punamäki ◽  
Marjo Flykt ◽  
Mervi Vänskä ◽  
Lotta M. Heikkilä ◽  
...  
Keyword(s):  
Emotion Regulation ◽  
Developmental Stage ◽  
Middle Childhood ◽  
Structural Equation ◽  
Developmental Plasticity ◽  
Structural Equation Models ◽  
Developmental Stages ◽  
Late Adolescence ◽  
Cognitive Emotion Regulation ◽  
Specific Effects

The quality of parenting shapes the development of children’s emotion regulation. However, the relative importance of parenting in different developmental stages, indicative of sensitive periods, has rarely been studied. Therefore, we formulated four hypothetical developmental timing models to test the stage-specific effects of mothering and fathering in terms of parental autonomy and intimacy in infancy, middle childhood, and late adolescence on adolescents’ emotion regulation. The emotion regulation included reappraisal, suppression, and rumination. We hypothesized that both mothering and fathering in each developmental stage contribute unique effects to adolescents’ emotion regulation patterns. The participants were 885 families followed from pregnancy to late adolescence. This preregistered study used data at the children’s ages of 1 year, 7 to 8 years, and 18 years. At each measurement point, maternal and paternal autonomy and intimacy were assessed with self- and partner reports using the Subjective Family Picture Test. At the age of 18 years, adolescents’ reappraisal and suppression were assessed using the Emotion Regulation Questionnaire and rumination using the Cognitive Emotion Regulation Questionnaire. Stage-specific effects were tested comparing structural equation models. Against our hypotheses, the results showed no effects of mothering or fathering in infancy, middle childhood, or late adolescence on adolescents’ emotion regulation patterns. The results were consistent irrespective of both the reporter (i.e., self or partner) and the parental dimension (i.e., autonomy or intimacy). In addition to our main results, there were relatively low agreement between the parents in each other’s parenting and descriptive discontinuity of parenting across time (i.e., configural measurement invariance). Overall, we found no support for the stage-specific effects of parent-reported parenting in infancy, middle childhood, or late adolescence on adolescents’ emotion regulation. Instead, our findings might reflect the high developmental plasticity of emotion regulation from infancy to late adolescence.

scholarly journals GATA1s exerts developmental stage-specific effects in human hematopoiesis

Haematologica ◽  
2018 ◽  
Vol 103 (8) ◽  
pp. e336-e340 ◽  
Author(s):  
Sofia Gialesaki ◽  
Anna Katharina Mahnken ◽  
Lena Schmid ◽  
Maurice Labuhn ◽  
Raj Bhayadia ◽  
...  
Keyword(s):  
Developmental Stage ◽  
Specific Effects

scholarly journals Oocyte batch development and enumeration in the European anchovy (Engraulis encrasicolus)

2016 ◽  
Vol 17 (3) ◽  
pp. 670 ◽  
Author(s):  
R. FERRERI ◽  
K. GANIAS ◽  
S. GENOVESE ◽  
I. FONTANA ◽  
G. GIACALONE ◽  
...  
Keyword(s):  
Batch Fecundity ◽  
Oocyte Size ◽  
Image Analysis Technique ◽  
Frequency Distributions ◽  
European Anchovy ◽  
Engraulis Encrasicolus ◽  
Hydrated Oocyte ◽  
Analysis Technique ◽  
Whole Mount ◽  
European Anchovy Engraulis Encrasicolus

An alternative method to the traditional hydrated oocyte (HO) method has been evaluated for the Sicilian anchovy, Engraulis encrasicolus. The method is based on the processing of ovarian whole mount images and the identification of the spawning batch in oocyte size frequency distributions and shows the advantage that it can be applied to various oocyte stages rather than strictly to the HO stage. Despite the peculiar elliptical shape of anchovy oocytes, this image analysis technique was fully successful since the yolked stage appeared to perform equally to the HO stage for anchovy batch fecundity measurements.

scholarly journals Drosophila melanogaster p53 has developmental stage-specific and sex-specific effects on adult life span indicative of sexual antagonistic pleiotropy

Aging ◽  
2009 ◽  
Vol 1 (11) ◽  
pp. 903-936 ◽  
Author(s):  
Morris Waskar ◽  
Gary N. Landis ◽  
Jie Shen ◽  
Christina Curtis ◽  
Kevin Tozer ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Life Span ◽  
Developmental Stage ◽  
Adult Life ◽  
Antagonistic Pleiotropy ◽  
Adult Life Span ◽  
Specific Effects

Alterations of ultrastructure and elemental composition in cultured human epidermal keratinocytes after treatment with nitrofurazone and silver sulfadiazine

1987 ◽  
Vol 45 ◽  
pp. 676-677
Author(s):  
A. R. Crooker ◽  
M. C. Myers ◽  
T. L. Beard ◽  
E. S. Graham
Keyword(s):  
Electron Microscopy ◽  
Elemental Composition ◽  
Pyrolytic Carbon ◽  
Human Keratinocytes ◽  
Silver Sulfadiazine ◽  
Epidermal Keratinocytes ◽  
Human Epidermal Keratinocytes ◽  
Culture Systems ◽  
Cytotoxicity Endpoints

Cell culture systems have become increasingly popular as a means of screening toxic agents and studying toxic mechanisms of drugs and other chemicals at the cellular and subcellular levels. These in vitro tests can be conducted rapidly in a broad range of relevant mammalian culture systems; a variety of biological and biochemical cytotoxicity endpoints can be examined. The following study utilized human keratinocytes to evaluate the relative cytotoxicities of nitrofurazone (NF) and silver sulfadiazine (SS), the active ingredients of FURACIN(R) Topical Cream and SILVADENE(R) Cream, respectively. These compounds are anti-infectives used in the treatment of burn patients. Cell ultrastructure and elemental composition were utilized as cytotoxicity endpoints.Normal Human Epidermal Keratinocytes (HK) were prepared from the EpiPackTM culture system (Clonetics Corporation, Boulder, CO). For scanning electron microscopy (SEM) and transmission electron microscopy (TEM), cells were seeded on sterile 35 mm Falcon plastic dishes; for elemental microanalysis, cells were plated on polished pyrolytic carbon discs (E. Fullam, Latham, NY) placed in the culture dishes.

Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

1986 ◽  
Vol 44 ◽  
pp. 134-135
Author(s):  
A. J. Tousimis
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Elemental Composition ◽  
Isotope Labeling ◽  
Structural Components ◽  
X Ray ◽  
Human Small Intestine ◽  
Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

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