scholarly journals Βacterial flora isolated from the oesophageal bulb of the olive fruit fly Dacus oleae (Gmelin)

2017 ◽  
Vol 6 ◽  
pp. 43 ◽  
Author(s):  
C. Stamopoulos ◽  
Ν. Μ. Tzanetakis

In a study of the bacterial flora occurring in the adults of the olive fruit fly, Dacus oleae (Gmelin) (Diptera: Tephritidae), oesophageal diverticulum, a total of 28 strains were obtained. Six of them were Gram – negative and identified as Pseudomonas niendocina, Morarella nonliquefasciens (2), Alcaligenes sp., Enterobacter cloacae (2) and 22 Gram-positive classified as Kurthia sp., Staphylococcus subgroup VI, Micrococcus roseus, Bacillus pumilus, B. licheniformis (3) and B. subtilis(15). None of the above bacteria are strictly fixed and constantly present in the oesophageal diverticulum, suggesting that the bacterial flora associated with D. oleae depends on environmental factors, and could be used as a nutrient source for the insect apart from its possible other symbiotic role.

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1053
Author(s):  
Stavroula A. Zisopoulou ◽  
Christina K. Chatzinikolaou ◽  
John K. Gallos ◽  
Anna Ofrydopoulou ◽  
Dimitra A. Lambropoulou ◽  
...  

Olive fruit fly Dacus oleae is a well-known pest infecting the bark of olive fruit, leading to reduction of extracted olive oil properties. Among chemicals proposed for Dacus oleae population control, pheromone 1,7-dioxaspiro(5.5)undecane (DSU), Dacus pheromone, is considered as a promising agent, which is added in several traps. However, all proposed systems manage to sufficiently deliver DSU for only two weeks. Furthermore, an additional problem is the limited available amount of pheromone to use in such systems. To overcome this, in the present study, a novel synthetic procedure of DSU is described, including only five steps. Intermediate products were studied by High Resolution Mass Spectroscopy Electrospray Ionization (HRMS-ESI) (m/z), while the resulting DSU was further characterized by 1H and 13C-NMR. Synthesized DSU was further encapsulated in poly(L-lactic acid) (PLLA) microparticles in three different concentrations; 5, 10 and 20% w/w. Its successful incorporation was studied by FT-IR, XRD and differential scanning calorimeter (DSC) while two procedures, liquid extraction and solid phase microextraction, followed by GC-MS analysis, was used for quantification of pheromone to microparticles. It was found that microparticles loading was over 85% for all three formulations. Its release showed a prolonged profile for microparticles containing 20% w/w DSU, lasting four weeks, while the quantity of DSU released reached 100%. These microparticles could be appropriate to control Dacus oleae population.


2010 ◽  
Vol 135 (5) ◽  
pp. 359-366 ◽  
Author(s):  
M. L. Pappas ◽  
G. D. Broufas ◽  
N. Koufali ◽  
P. Pieri ◽  
D. S. Koveos

1968 ◽  
Vol 12 (1) ◽  
pp. 1-9 ◽  
Author(s):  
E. Zouros ◽  
S. Tsakas ◽  
C. B. Krimbas

The genetics of two esterase systems in adults of the olive fruit fly Dacus oleae (Gmel.) have been studied by starch gel electrophoresis. Two unlinked autosomal genes, each with multiple alleles, control the synthesis of a number of isozymes. Gene A controls the synthesis of an acetylcholinesterase, and gene B that of a pseudocholinesterase or lipase. Gene B produces hybrid enzyme in heterozygotes for active alleles. In crosses the segregation of seven active and one silent allele in gene A, and of six active and one silent allele in gene B, have been studied. Six more active alleles for gene A and five active alleles of gene B have not been studied in this paper.


2009 ◽  
Vol 93 (1-5) ◽  
pp. 463-476 ◽  
Author(s):  
A. P. Economopoulos ◽  
G. E. Haniotakis ◽  
S. Michelakis ◽  
G. J. Tsiropoulos ◽  
G. A. Zervas ◽  
...  

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