scholarly journals Some enzymatic activities registered in eggs and gut tissues of the olive fruit fly, Dacus oleae (Gmelin)

2017 ◽  
Vol 6 ◽  
pp. 23 ◽  
Author(s):  
C. Stamopoulos
Keyword(s):  
Amino Acids ◽  
Alkaline Phosphatase ◽  
Fruit Fly ◽  
Enzymatic Activities ◽  
Olive Fruit Fly ◽  
Enzymatic System ◽  
Dacus Oleae ◽  
Essential Nutrients ◽  
Clear Effect ◽  
Different Content

Nineteen enzymatic activities of eggs, gut tissues of larvae and adults of Dacus oleae have been determined using the API-ZYM micromethod. The enzymatic activities of eggs were much weaker than those of larval or adult guts. The activities increased progressively to the L2 and L3. The lack of feeding caused a reduction of certain activities in larvae (alkaline phosphatase, esterases, aminopeptidases) but not in adults. The addition of streptomycin to the food of adults, to obtain “aposymbiotic” individuals, did not have a clear effect on the activities of the insect’s various stages. Although the enzymes studied were not the only ones that occur in the insect’s gut tissues, it seems that there are few similarities between the enzymatic system of larvae and that of adults, most probably because of the different content of the two diets in essential nutrients such as amino acids, proteins, and lipids.

scholarly journals The genetics of Dacus oleae: III. Amount of variation at two esterase loci in a Greek population

1969 ◽  
Vol 14 (3) ◽  
pp. 249-258 ◽  
Author(s):  
E. Zouros ◽  
C. B. Krimbas
Keyword(s):  
Allele Frequency ◽  
Fruit Fly ◽  
Olive Fruit Fly ◽  
Allele Frequency Distribution ◽  
Natural Conditions ◽  
Dacus Oleae ◽  
Random Drift ◽  
Selective Neutrality ◽  
Esterase Loci ◽  
Silent Allele

Two polymorphic esterase loci, EstA and EstB, of the olive-fruit fly Dacus oleae were studied in a natural population. The analysis of about 500 individuals revealed the presence of 15 alleles for EstA and 12 alleles for EstB. A ‘silent’ allele was found segregating at both loci. Segregation data for most of the alleles are presented. The allele frequency distribution follows the same pattern at both loci: one allele of each gene has a frequency of nearly 0·50, a few have frequencies between 0·05 and 0·15 and many are below 0·05. Two main hypotheses, those of overdominance and selective neutrality, were examined in order to explain these polymorphisms. We deduced that under both hypotheses a relatively high mutation rate is necessary to balance the result of random drift. This rate was estimated to be higher than 4 × 10−5 for the EstA locus. Since homozygotes for the ‘silent’ allele at the first or at the second locus were found in the population in expected frequencies, it was concluded that these alleles are not inferior to active ones under natural conditions.

scholarly journals Synthesis of Dacus Pheromone, 1,7-Dioxaspiro[5.5]Undecane and Its Encapsulation in PLLA Microspheres for Their Potential Use as Controlled Release Devices

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1053
Author(s):  
Stavroula A. Zisopoulou ◽  
Christina K. Chatzinikolaou ◽  
John K. Gallos ◽  
Anna Ofrydopoulou ◽  
Dimitra A. Lambropoulou ◽  
...  
Keyword(s):  
Solid Phase ◽  
Population Control ◽  
Fruit Fly ◽  
Olive Fruit Fly ◽  
Dacus Oleae ◽  
1H And 13C Nmr ◽  
Olive Fruit ◽  
Synthetic Procedure ◽  
Ft Ir ◽  
Release Devices

Olive fruit fly Dacus oleae is a well-known pest infecting the bark of olive fruit, leading to reduction of extracted olive oil properties. Among chemicals proposed for Dacus oleae population control, pheromone 1,7-dioxaspiro(5.5)undecane (DSU), Dacus pheromone, is considered as a promising agent, which is added in several traps. However, all proposed systems manage to sufficiently deliver DSU for only two weeks. Furthermore, an additional problem is the limited available amount of pheromone to use in such systems. To overcome this, in the present study, a novel synthetic procedure of DSU is described, including only five steps. Intermediate products were studied by High Resolution Mass Spectroscopy Electrospray Ionization (HRMS-ESI) (m/z), while the resulting DSU was further characterized by 1H and 13C-NMR. Synthesized DSU was further encapsulated in poly(L-lactic acid) (PLLA) microparticles in three different concentrations; 5, 10 and 20% w/w. Its successful incorporation was studied by FT-IR, XRD and differential scanning calorimeter (DSC) while two procedures, liquid extraction and solid phase microextraction, followed by GC-MS analysis, was used for quantification of pheromone to microparticles. It was found that microparticles loading was over 85% for all three formulations. Its release showed a prolonged profile for microparticles containing 20% w/w DSU, lasting four weeks, while the quantity of DSU released reached 100%. These microparticles could be appropriate to control Dacus oleae population.

Effect of heat stress on survival and reproduction of the olive fruit fly Bactocera (Dacus) oleae

2010 ◽  
Vol 135 (5) ◽  
pp. 359-366 ◽  
Author(s):  
M. L. Pappas ◽  
G. D. Broufas ◽  
N. Koufali ◽  
P. Pieri ◽  
D. S. Koveos
Keyword(s):  
Heat Stress ◽  
Fruit Fly ◽  
Olive Fruit Fly ◽  
Dacus Oleae ◽  
Olive Fruit ◽  
Survival And Reproduction

scholarly journals The genetics of Dacus oleae: II. The genetics of two adult esterases

1968 ◽  
Vol 12 (1) ◽  
pp. 1-9 ◽  
Author(s):  
E. Zouros ◽  
S. Tsakas ◽  
C. B. Krimbas
Keyword(s):  
Gel Electrophoresis ◽  
Fruit Fly ◽  
Olive Fruit Fly ◽  
Starch Gel Electrophoresis ◽  
Dacus Oleae ◽  
Lipase Gene ◽  
Multiple Alleles ◽  
Olive Fruit ◽  
Starch Gel ◽  
Silent Allele

The genetics of two esterase systems in adults of the olive fruit fly Dacus oleae (Gmel.) have been studied by starch gel electrophoresis. Two unlinked autosomal genes, each with multiple alleles, control the synthesis of a number of isozymes. Gene A controls the synthesis of an acetylcholinesterase, and gene B that of a pseudocholinesterase or lipase. Gene B produces hybrid enzyme in heterozygotes for active alleles. In crosses the segregation of seven active and one silent allele in gene A, and of six active and one silent allele in gene B, have been studied. Six more active alleles for gene A and five active alleles of gene B have not been studied in this paper.

Population studies on the olive fruit fly, Dacus oleae (Gmel.) (Dipt., Tephritidae) in Western Crete

2009 ◽  
Vol 93 (1-5) ◽  
pp. 463-476 ◽  
Author(s):  
A. P. Economopoulos ◽  
G. E. Haniotakis ◽  
S. Michelakis ◽  
G. J. Tsiropoulos ◽  
G. A. Zervas ◽  
...  
Keyword(s):  
Population Studies ◽  
Fruit Fly ◽  
Olive Fruit Fly ◽  
Dacus Oleae ◽  
Olive Fruit
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