scholarly journals Contribution to the study of potato aphids in Greece

2017 ◽  
Vol 4 ◽  
pp. 11 ◽  
Author(s):  
P.C. Panayotoy ◽  
N. Katis
Keyword(s):  
Potato Virus ◽  
High Resistance ◽  
Potato Virus Y ◽  
Aphid Species ◽  
Potato Plants ◽  
Potato Foliage ◽  
Potato Aphids ◽  
First Time

Four aphid species were identified in the Metsovo potato center, Greece. Rhopalosiphoninus latysiphon, infesting subterranean parts of potato, is reported for the first time in Greece, while Aphis frangulae, infesting the potato foliage, is reported for the first time on potato plants in Greece. High resistance to methamidophos was observed with the latter aphid species. Potato virus Y (PVY) was transmitted by all four species of aphids

Cytological responses of susceptible and extremely resistant potato plants to inoculation with potato virus Y

1999 ◽  
Vol 55 (3) ◽  
pp. 143-150 ◽  
Author(s):  
J. HINRICHS-BERGER ◽  
M. HARFOLD ◽  
S. BERGER ◽  
H. BUCHENAUER
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Plants

scholarly journals Detection and genome characterization of Potato virus Y isolates infecting potato (Solanum tuberosum L.) in La Union (Antioquia, Colombia)

2016 ◽  
Vol 34 (3) ◽  
pp. 317-328 ◽  
Author(s):  
Pablo Gutiérrez S. ◽  
Mauricio Marín M. ◽  
Daniel Muñoz E.
Keyword(s):  
Solanum Tuberosum ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Seed Tuber ◽  
Rt Pcr ◽  
Potato Plants ◽  
Nextgeneration Sequencing ◽  
Prevalent Strain ◽  
Detailed Molecular Analysis

Potato virus Y (PVY) is one of the most severe viruses affecting the production of potato (Solanum tuberosum) in the world. This study presents a detailed molecular analysis using nextgeneration sequencing (NGS), IC-RT-qPCR and RT-PCR on the PVY isolates infecting seed-tubers and foliage of potato plants cv. Diacol-Capiro in La Union (Antioquia, Colombia). Analysis of incidence by IC-RT-qPCR in 15 random leaf samples of three cultivation plots and fifteen sprouting tuber eye-buds reveal infection levels between 13.4 and 80%; a higher incidence of 86.7% was observed in seed-tuber samples with threshold cycle (Ct) values as low as 24.3. Genome assembly from a bulk of foliage samples resulted in a consensus PVY genome (PVY_LaUnionF) of 9,702 nt and 399 polymorphic sites within the polyprotein ORF; while the assembled genome from sprouts of tubers has 9,704 nt (PVY_LaUnionT) and contained only six polymorphic nucleotide sites. Phylogenetic analysis demonstrates that the PVY isolates from leaf samples are in the recombinant PVYNTN group (sequence identity >99%); while those from tuber sprouts are in the PVYN/NTN group with identities above 95%. Sanger sequencing of viral capsid suggests the presence of a third variant related to PVYO, a prevalent strain reported in potato fields worldwide.

scholarly journals Role of the methionine cycle in the temperature‐sensitive responses of potato plants to potato virus Y

2020 ◽  
Vol 22 (1) ◽  
pp. 77-91 ◽  
Author(s):  
Igor Fesenko ◽  
Nadezhda Spechenkova ◽  
Anna Mamaeva ◽  
Antonida V. Makhotenko ◽  
Andrew J. Love ◽  
...  
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Temperature Sensitive ◽  
Potato Plants ◽  
Methionine Cycle

Quantification of mineral oil accumulation and movement in potato plants and its significance in potato virus Y management

2013 ◽  
Vol 70 (8) ◽  
pp. 1243-1248 ◽  
Author(s):  
Manphool Fageria ◽  
Sébastien Boquel ◽  
Gaetan Leclair ◽  
Yvan Pelletier
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Mineral Oil ◽  
Oil Accumulation ◽  
Potato Plants

scholarly journals Modulation of Aphid Vector Activity by Potato virus Y on In Vitro Potato Plants

Plant Disease ◽  
2012 ◽  
Vol 96 (1) ◽  
pp. 82-86 ◽  
Author(s):  
S. Boquel ◽  
C. Delayen ◽  
A. Couty ◽  
P. Giordanengo ◽  
A. Ameline
Keyword(s):  
Feeding Behavior ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Aphid Species ◽  
Aphis Fabae ◽  
Plant Colonization ◽  
Electrical Penetration Graph ◽  
Vector Activity ◽  
Aphid Feeding

The effects of the infection of potato (Solanum tuberosum) plants by the nonpersistent Potato virus Y (PVY) were studied on the host plant colonization behavior of different colonizing (Myzus persicae) and noncolonizing (Aphis fabae, Brevicoryne brassicae, and Sitobion avenae) aphid species. The underlying questions of this study were to know how aphids respond when faced with PVY-infected plants and whether plant infection can modify the aphid behavior involved in PVY spread. Short-range orientation behavior was observed using a dual-choice set-up and aphid feeding behavior was monitored using the electrical penetration graph technique. None of the aphid species discriminated between healthy and PVY-infected plants. Nevertheless, most individuals of M. persicae landed on and probed only in one plant whereas noncolonizing aphid species exhibited interplant movements. Study of the aphid feeding behavior showed that PVY infection essentially modified phloem and xylem ingestion. M. persicae and S. avenae exhibited an increased duration of phloem phases on PVY-infected plants whereas A. fabae showed a decreased duration of phloem phases that benefited from an increased duration of xylem ingestion phases. None of these parameters were changed in B. brassicae. These data present evidence that aphids can respond to plants infected by nonpersistent viruses. Such behavioral modifications are discussed within the context of PVY spread in potato crops.

scholarly journals Occurrence and Molecular Characterization of Recombinant Potato virus YNTN Isolates from Sulawesi, Indonesia

Plant Disease ◽  
2016 ◽  
Vol 100 (2) ◽  
pp. 269-275 ◽  
Author(s):  
Mohamad Chikh-Ali ◽  
Nilsa A. Bosque-Pérez ◽  
Dalton Vander Pol ◽  
Dantje Sembel ◽  
Alexander V. Karasev
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Cultivar ◽  
Whole Genome ◽  
Chain Reaction ◽  
Recombinant Type ◽  
Whole Genomes ◽  
Polymerase Chain ◽  
First Time

The importance of potato has increased dramatically in Indonesia over the last three decades. During this period, ‘Granola’, a potato cultivar originally from Germany, has become the most common cultivar for fresh consumption in Indonesia. In August 2014, a survey was conducted in Sulawesi, where potato fields cultivated with Granola and its selection, ‘Super John’, were sampled for Potato virus Y (PVY) presence. PVY was found in Sulawesi for the first time. Samples determined to be positive for PVY were subsequently typed to strain using reverse-transcription polymerase chain reaction assays. All PVY isolates sampled were identified as PVYNTN recombinants, with three recombination junctions in P3, VPg, and CP regions of the genome. Three local PVY isolates were subjected to whole-genome sequencing and subsequent sequence analysis. The whole genomes of the Indonesian PVYNTN isolates I-6, I-16, and I-17 were found to be closely related to the European PVYNTN-A. This recombinant type was shown previously to cause potato tuber necrotic ringspot disease (PTNRD) in susceptible potato cultivars. The dependence of potato farmers on mostly a single cultivar, Granola, may have given a competitive advantage to PVYNTN over other PVY strains, resulting in the predominance of the PVYNTN recombinant. The dominance of PVYNTN in Sulawesi, and possibly in Indonesia as a whole, represents a potential risk to any newly introduced potato cultivar to the country, especially cultivars susceptible to PTNRD.

scholarly journals An Improved Multiplex IC-RT-PCR Assay Distinguishes Nine Strains of Potato virus Y

Plant Disease ◽  
2013 ◽  
Vol 97 (10) ◽  
pp. 1370-1374 ◽  
Author(s):  
Mohamad Chikh-Ali ◽  
Stewart M. Gray ◽  
Alexander V. Karasev
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Rna Extraction ◽  
Leaf Tissue ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Potato Leaf ◽  
Extraction Step ◽  
First Time ◽  
Virus Testing

A multiplex reverse-transcription polymerase chain reaction (RT-PCR) assay was previously developed to identify a group of Potato virus Y (PVY) isolates with unusual recombinant structures (e.g., PVYNTN-NW and SYR-III) and to differentiate them from other PVY strains. In the present study, the efficiency of this multiplex RT-PCR assay was validated and extended considerably to include five additional strains and strain groups not tested before. To make the multiplex RT-PCR assay more applicable and suitable for routine virus testing and typing, it was modified by replacing the conventional RNA extraction step with the immunocapture (IC) procedure. The results obtained using well-characterized reference isolates revealed, for the first time, that this multiplex RT-PCR assay is an accurate and robust method to identify and differentiate the nine PVY strains reported to date, including PVYO (both PVYO and PVYO-O5), PVYN, PVYNA-N, PVYNTN, PVYZ, PVYE, PVY-NE11, PVYN-Wi, and PVYN:O, which is not possible by any of the previously reported RT-PCR procedures. This would make the IC-RT-PCR procedure presented here a method of choice to identify PVY strains and assess the strain composition of PVY in a given area. The IC-RT-PCR protocol was successfully applied to typing PVY isolates in potato leaf tissue collected in the field.

Role of Hairy Nightshade in the Transmission of Different Potato virus Y Strains on Solanum tuberosum (L.)

2010 ◽  
Vol 11 (1) ◽  
pp. 38 ◽  
Author(s):  
Felix A. Cervantes ◽  
Juan M. Alvarez
Keyword(s):  
Potato Virus ◽  
Potato Virus Y ◽  
Green Peach Aphid ◽  
Transmission Rate ◽  
Solanum Tuberosum L ◽  
Macrosiphum Euphorbiae ◽  
Invasive Weed ◽  
Potato Plants ◽  
Tuber Necrosis

The complexity of the Potato virus Y (PVY) (Potyviridae: Potyvirus) pathosystem is affected by the presence of several virus strains that differ in their ability to produce tuber necrosis and by the presence of an alternate host that could increase the amount of inoculum in potato fields. Solanum sarrachoides (Sendtner) is an invasive weed from South America present in Pacific Northwest potato agro-ecosystems. It serves as reservoir of PVY and its most efficient vectors: the green peach aphid, Myzus persicae (Sulzer), and the potato aphid, Macrosiphum euphorbiae (Thomas). The role of S. sarracoides as vector and virus reservoir in PVY epidemiology was investigated through a series of laboratory and greenhouse experiments. We studied the symptoms produced in S. sarracoides upon infection with necrotic and non-necrotic strains of PVY and looked at the percentage of infection and titer accumulation of these strains. PVY infection in S. sarrachoides produced symptoms similar to those produced in PVY-infected potato plants. Mottling and yellowing were the main symptoms of infection observed in S. sarrachoides plants, especially by PVYO and PVYNTN infection. Greenhouse transmission studies revealed that PVY-infected S. sarrachoides increased the transmission rate of PVY necrotic strains by M. persicae. The necrotic strain PVYNTN reached higher titer in S. sarrachoides than in potato plants when compared to PVYO and PVYN:O These findings have broadened our understanding of the role and importance of S. sarrachoides in the PVY epidemiology in the potato ecosystems and could potentially be included in the development or optimization of virus management programs. Accepted for publication 15 March 2010. Published 26 May 2010.

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