scholarly journals Η πιθανή ευεργετική δράση αυξητικών παραγόντων στην επούλωση πειραματικά δημιουργηθείσας και εκταμθείσας ενδομητριωσικής εστίας στο παχύ έντερο επιμύων

2019 ◽  
Author(s):  
Αναστασία Προδρομίδου
Keyword(s):  
Platelet Rich Plasma ◽  
Sprague Dawley ◽  
Baxter Healthcare

Σκοπός: Η αξιολόγηση της πιθανής ευεργετικής δρασης του πλάσματος εμπλουτισμένου με αιμοπετάλια (Platelet rich plasma-PRP) και του συγκολλητικού παράγοντα ινώδους (TISSEEL, Baxter Healthcare Corporation, Deerfield, IL) στην επούλωση του εντέρου μετά το “ξύρισμα”-εφαπτομενική εκτομή πειραματικά επαγόμενης ενδομητριωτικής εστίας παχέος εντέρου. Σχεδίαση μελέτης: Τυφλή τυχαιοποιημένη πειραματική μελέτη. Ζώα: Τριάντα θήλεις αρουραίοι Sprague-Dawley (SD). Παρεμβάσεις: Επαγωγή πειραματικής ενδομητρίωσης του παχέος εντέρου με αυτόλογη μεταμόσχευση ενδομητρικού ιστού σε όλα τα ζώα (1η χειρουργική επέμβαση). Τριάντα αρουραίοι τυχαιοποιήθηκαν σε 1 από τις 3 ομάδες σύμφωνα με την θεραπευτική παρέμβαση: PRP (ομάδα I, ν = 10), TISSEEL (ομάδα II, n = 10) ή κανένας παράγοντας (ομάδα III, ν=10) εφαρμόστηκαν στην “ξυρισμένη” επιφάνεια του παχέος εντέρου (2η χειρουργική επέμβαση). Μετρήσεις και κύρια αποτελέσματα: Η ενδομητρίωση του παχέος εντέρου επάχθηκε με επιτυχία σε όλα τα ζώα. Τέσσερις ημέρες μετά τη 2η χειρουργική επέμβαση, τα ζώα υπέστησαν ευθανασία και πραγματοποιήθηκε ιστολογική εξέταση. Η ιστολογική εξέταση έδειξε ότι σε σύγκριση με την ομάδα ελέγχου, η εναπόθεση κολλαγόνου βρέθηκε σε σημαντικά υψηλότερη έκφραση τόσο στην ομάδα του PRP όσο και σε αυτή του TISSEEL (p = .011 και p = .011, αντίστοιχα). Η διαταραχή της αρχιτεκτονικής των στιβάδων του παχεός εντέρου ήταν στατιστικά πιο έντονη στην ομάδα ελέγχου συγκριτικά με την ομάδα TISSEEL (p = .033) ενώ παρατηρήθηκε πιο εξεσημασμένος σχηματισμός νέων αιμοφόρων αγγείων στην ομάδα του TISSEEL σε σύγκριση με την ομάδα ελέγχου (p = .023). Δεν ανιχνεύθηκαν ιστολογικές ενδείξεις υπολειμματικής ή υποτροπιάζουσας νόσου. Συμπέρασμα: Τόσο η χρήση του PRP όσο και του TISSEEL φαίνεται να είναι ασφαλής και να σχετίζεται με βελτίωση της επούλωσης των ιστών μετά την αποψίλωση ενδομητριωσικής εστίας παχέος εντέρου, που αποδίδεται στην αυξημένη εναπόθεση κολλαγόνου, στη νεοαγγείωση και την προστασία της ακεραιότητας των στιβάδων του παχέος εντέρου. Στο πεδίο αυτό, κλινικές δοκιμές απαιτούνται για να επιβεβαιώσουν την αποτελεσματικότητα του PRP και του TISSEEL στην κλινική πράξη.

scholarly journals Effect of Combined Leukocyte-Poor Platelet-Rich Plasma and Hyaluronic Acid on Bone Marrow–Derived Mesenchymal Stem Cell and Chondrocyte Metabolism

Cartilage ◽  
2019 ◽  
pp. 194760351985873 ◽  
Author(s):  
Alexander M. Satin ◽  
Jolanta B. Norelli ◽  
Nicholas A. Sgaglione ◽  
Daniel A. Grande
Keyword(s):  
Bone Marrow ◽  
Hyaluronic Acid ◽  
Cellular Proliferation ◽  
Culture Media ◽  
Platelet Rich Plasma ◽  
Interleukin 1 ◽  
Cellular Growth ◽  
Sprague Dawley ◽  
Vitro Effect

ObjectiveGiven the potential applications of combined biologics, the authors sought to evaluate the in vitro effect of combined platelet-rich plasma (PRP) and hyaluronic acid (HA) on cellular metabolism.DesignBone marrow–derived mesenchymal stem cells (BMSCs) and chondrocytes were obtained from the femurs of Sprague-Dawley rats. An inflammatory model was created by adding 10 ng/mL interleukin-1-beta to culture media. Non-crosslinked high-molecular-weight HA, activated-PRP (aPRP), and unactivated-PRP (uPRP) were tested. Cellular proliferation and gene expression were measured at 1 week. Genes of interest included aggrecan, matrix metalloproteinase (MMP)-9, and MMP-13.ResultsCombined uPRP-HA was associated with a significant increase in chondrocyte and BMSC proliferation at numerous preparations. There was a trend of increased chondrocyte aggrecan expression with combined PRP-HA. The greatest and only significant decrease in BMSC MMP-9 expression was observed with combined PRP-HA. While a significant reduction of BMSC MMP-13 expression was seen with PRP and HA-alone, a greater reduction was observed with PRP-HA. MMP-9 chondrocyte expression was significantly reduced in cells treated with PRP-HA. PRP-alone and HA-alone at identical concentrations did not result in a significant reduction. The greatest reduction of MMP-13 chondrocyte expression was observed in chondrocytes plus combined PRP-HA.ConclusionsWe demonstrated a statistically significant increase in BMSC and chondrocyte proliferation and decreased expression of catabolic enzymes with combined PRP-HA. These results demonstrate the additive in vitro effect of combined PRP-HA to stimulate cellular growth, restore components of the articular extracellular matrix, and reduce inflammation.

scholarly journals In vivo study of wound healing processes in Sprague-Dawley model using human mesenchymal stem cells and platelet-rich plasma

2021 ◽  
Vol 8 (4) ◽  
pp. 4315-4323
Author(s):  
Karina Karina ◽  
Johannes Albert Biben ◽  
Krista Ekaputri ◽  
Imam Rosadi ◽  
Iis Rosliana ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Mesenchymal Stem Cells ◽  
Cell Differentiation ◽  
Histological Analysis ◽  
Platelet Rich Plasma ◽  
Sprague Dawley ◽  
Burn Wound ◽  
Wound Model ◽  
Healing Processes

Introduction: Mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) have a potential role in improving wound healing processes. This experimental study aims to compare PRP and MSCs to promote the wound healing process in the animal burn wound model. Methods: PRP from venous blood and MSCs from lipoaspirates were isolated from six donors. Saline solution was used as control while PRP and MSCs as treatment groups were injected to second-degree burn wounds in the backs of 10 male Sprague-Dawley rats for each group. On day 7, 5 rats from each group were euthanized for analyses VEGF gene, which has a role in angiogenesis. At the end of the study (week 5), the remaining rats were euthanized for histological analysis. Results: The VEGF expression in MSCs and PRP groups was higher than the control group (not significant). The wound healing rate was also significantly faster until 21 days post-burn in the MSCs and PRP groups and getting slowly afterward. Histological analysis showed the burned skin at day 35 had displayed the best differentiation outcome in the MSCs group. In conclusion, human-derived MSCs and PRP do not accelerate epithelialization duration of rats burn wound model. However, they improved wound’s vascularization and cell differentiation. Conclusion: MSCs are superior to PRP in enhancing cell differentiation.

In Vitro and In Vivo Inhibitory Effects of Alcohol on Platelet Functions of Rats Fed Dietary Saturated Fats.

1979 ◽  
Author(s):  
L. McGregor ◽  
S. Renaud
Keyword(s):  
Fatty Acids ◽  
Drinking Water ◽  
Corn Oil ◽  
Platelet Rich Plasma ◽  
Saturated Fatty Acids ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Platelet Functions

In the in vitro experiment, alcohol diluted in complete tyrode wae added, at 37°c, 2min before aggregation tests, to platelet-rich plasma (final dilution in plasma : 0.00016 and 0.00032%) from male, Sprague-Dawley rats. These animals were fed either laboratory chow or a high fat (40%) purified diet rich in either polyunsaturated fatty acids (22% corn oil) or in long chain saturated fatty acids (38% with 2% corn oil). Aggregation to thrombin but not to ADP was significantly reduced (50%) in all 3 groups of rats with 0.00032% alcohol, even in hyperaggregable animals fed saturated fats. Addition of 0.00016% alcohol slightly reduced platelet response to thrombin. The in vivo experiment consisted of feeding 48 weanling male Sprague-Dawley rats with purified diets, as mentioned above, rich in either polyunsaturated fatty acids or saturated fatty acids for at least 7 months. Morever, half of these animals had 6% alcohol in their drinking water for at least 2 months. Addition of alcohol, in drinking water, significantly prolonged platelet-rich plasma clotting time of saturated fat (101vs 136 sec) and in polyunsaturated fat group of animals (130vs 145 sec). Platelet maximal response of aggregation to thrombin (7.2vs 4.0 cm) and to ADP (9.0vs 5.7 cm) were significantly reduced by alcohol. Alcohol, in drinking water, appears to markedly inhibit platelet functions in rat. This seems to result from a direct effect on blood platelets since it can be partly reproduced by adding alcohol co platelet-rich plasma in vitro.

In Vitro Effect of Ethanol on ADP and Collagen-Induced Platelet Aggregation

1981 ◽  
Vol 46 (04) ◽  
pp. 673-675 ◽  
Author(s):  
Stig Bengmark ◽  
Olle Elmér ◽  
Göran Göransson ◽  
Evita Zoucas
Keyword(s):  
Platelet Aggregation ◽  
Ethanol Concentration ◽  
Platelet Rich Plasma ◽  
Rat Plasma ◽  
Rabbit Plasma ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Vitro Effect ◽  
Induced Aggregation

SummaryPlatelet aggregation was measured by Born’s method. Plasma from Sprague-Dawley rats and inbred albino rabbits was used. 20% ethanol in 0.9%(w/v) NaCl was added to plasma at final concentrations of 56.5 mM, 85.9 mM 171.5 mM and 343.1 mM or to whole blood at final concentrations of 8.7 mM, 17.4 mM, 52.5 mM, 171.5 mM and 343.1 mM. Experiments using 0.9% NaCl volumes equivalent to the added ethanol volume were also conducted. Dose-response aggregation tests showed that a decrease in ADP-and collagen-induced aggregation existed when ethanol was mixed with rat or rabbit blood prior to the preparation of platelet-rich plasma. There was no effect on ADP-induced platelet aggregation when ethanol was mixed with rat or rabbit plasma. Collagen-induced aggregation was impaired only in rat plasma when ethanol concentration reached 343.1 mM. These results suggested that ethanol modified platelet function possibly via red cells.

scholarly journals Nestin expressions of exposed pulp after direct pulp capping by calcium hydroxide and platelet rich plasma

2016 ◽  
Vol 10 (03) ◽  
pp. 341-344 ◽  
Author(s):  
Sartika Puspita ◽  
Totok Utoro ◽  
Tetiana Haniastuti
Keyword(s):  
Calcium Hydroxide ◽  
Platelet Rich Plasma ◽  
Sprague Dawley ◽  
Direct Pulp Capping ◽  
Nestin Expression ◽  
Pulp Tissue ◽  
Pulp Capping ◽  
Sprague Dawley Rats ◽  
Group 2 ◽  
Immunohistochemical Techniques

ABSTRACT Objective: The aim of this study was to evaluate nestin expression of pulp tissue following direct pulp capping with platelet-rich plasma (PRP). Materials and Methods: The thirty sound teeth from Sprague-Dawley rats were used and divided into two groups: Groups 1, teeth were capped with calcium hydroxide/Ca(OH)2 (n = 15) and Group 2 with PRP (n = 15). After 1st, 7th, and 21st days, respectively, 5 teeth each group (American Dental Association 41) were processed for light microscopic examination. Expressions of nestin were assessed by immunohistochemical techniques. Results: Nestin expression of Ca(OH)2 on the distance place of exposure at 1st and 7th days were 80% and at 21st day were 60%. Nestin expression of PRP on the distance place at 1st day was 80%, 7th 100%, and 21st day was 80%. At day 21 observation, Kruskal–Wallis test shows nestin expression was increased significantly in PRP groups (P < 0.05), but it was not increase significantly compare with Ca(OH)2. Conclusion: PRP had ability as a direct pulp capping material to induce nestin expression.

scholarly journals Effects of Varying Concentrations of Dietary Linoleic Acid on Platelet-Rich Plasma Clotting Time, Platelet Aggregation and Phospholipid Fatty Acids Composition of Rats

1977 ◽  
Author(s):  
L. McGregor ◽  
S. Renaud
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Platelet Aggregation ◽  
Corn Oil ◽  
Platelet Rich Plasma ◽  
Coconut Oil ◽  
Phospholipid Fatty Acids ◽  
Sprague Dawley ◽  
Clotting Time ◽  
Dietary Linoleic Acid

A high intake of saturated fats has been shown, in rats fed drastic diets, to be associated with an acceleration of platelet-rich plasma clotting time, an increased thrombin aggregation and a high incidence of venous thrombosis. Linoleic acid appeared to have an antithrombotic effect. In order to understand the role of dietary linoleic acid in platelet function, the present study was undertaken with different concentrations of linoleic acid (0%, 0.5%, 1% and 6%), provided by corn oil which were added to purified diet high in saturated fat (40% hydro-genated coconut oil) but otherwise completely adequate in any other aspects. These four diets were fed to 4 groups of weanling male Sprague-Dawley rats for 4 months. At the end of the feeding period, platelet-rich plasma clotting time, platelet aggregation to thrombin, ADP, arachidonate, collagen and fatty acid composition of total platelet phospholipids were determined. Rats fed 0%, 0.5%, 1% linoleic acid showed a marked hypercoagulability, hyperagregability to thrombin, in comparison to 6% linoleic acid-fed rats. Moreover, this hyperagregability and hypercoagulability were associated with the following increased ratios of platelet phospholipid fatty acids : 20 : 4/18 : 2 and 16 : 1 + 18 : 1 + 20 : 1 + 24: 1 + 20 : 3ω9/18 : 2. It seems that dietary linoleic acid plays a crucial role in platelet functions.

Platelet-Rich Plasma Releasate Promotes Regeneration and Decreases Inflammation and Apoptosis of Injured Skeletal Muscle

2018 ◽  
Vol 46 (8) ◽  
pp. 1980-1986 ◽  
Author(s):  
Wen-Chung Tsai ◽  
Tung-Yang Yu ◽  
Gwo-Jyh Chang ◽  
Li-Ping Lin ◽  
Miao-Sui Lin ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Apoptosis ◽  
Muscle Injury ◽  
Animal Studies ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Sprague Dawley ◽  
And Migration

Background: Platelet-rich plasma (PRP) contains various cytokines and growth factors that may be beneficial to the healing process of injured muscle. Based on the authors’ previous study, PRP releasate can promote proliferation and migration of skeletal muscle cells in vitro, so animal studies are performed to support the use of PRP to treat muscle injury in vivo. Purpose: To investigate the effect of PRP releasate on regeneration of injured muscle, as well as its effect on inflammatory reaction and cell apoptosis, in the early stages of the muscle-healing process. Study Design: Controlled laboratory study. Methods: The gastrocnemius muscles of Sprague-Dawley rats were injured by partial transverse incision and then treated with PRP releasate. Hematoxylin and eosin stain was used to evaluate the healing process of injured muscle at 2, 5, and 10 days after injury. TUNEL assay was used to evaluate the cell apoptosis of injured muscle after PRP releasate treatment. Immunohistochemistry was used to stain the CD68-positive cells during the healing process. Muscle contractile properties, including fast-twitch and tetanic strength, were evaluated by electric stimulation. Results: The results revealed that PRP releasate treatment could enhance the muscle-healing process and decrease CD68-positive cells and apoptotic cells. Furthermore, the tetanic strength was significantly higher in injured muscle treated with PRP releasate. Conclusion: In conclusion, PRP releasate could enhance the healing process of injured muscle and decrease inflammatory cell infiltration as well as cell apoptosis. Clinical Relevance: PRP promotes skeletal muscle healing in association with decreasing inflammation and apoptosis of injured skeletal muscle. These findings provide in vivo evidence to support the use of PRP to treat muscle injury.

Agonist-Induced Release of Neuropeptide Y by Platelets

1996 ◽  
Vol 9 (1) ◽  
pp. 1-8
Author(s):  
A. Torres Duarte ◽  
Z. Zukowska-Grojec ◽  
A.K. Myers
Keyword(s):  
Platelet Aggregation ◽  
Immune Responses ◽  
Neuropeptide Y ◽  
Platelet Rich Plasma ◽  
High Dose ◽  
Dependent Manner ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Irreversible Aggregation ◽  
Dose Dependent

Neuropeptide Y (NPY) is a multi-functional peptide localized in a number of tissues besides the brain, and its expression is high in strains of mice with autoimmune disease. Functionally, along with its neurotransmitter role, NPY has multiple effects on both vascular tissues and tissues involved in immune responses. Previous studies demonstrated that immunoreactive NPY (i-NPY) exists in large quantities in platelets and bone marrow of some species, and suggested its release during platelet aggregation by collagen. Because NPY has been hypothesized to have a role in a number of pathophysiological states in which platelets are involved, including immune responses, hypertension and vascular smooth muscle proliferation, we sought to further characterize platelet i-NPY content and release by a diverse group of physiological platelet agonists. Platelet rich plasma and gel filtered platelets were prepared from citrated whole blood of male Sprague Dawley rats. Platelet content and concentration of i-NPY were quantified by RIA. Platelet aggregation responses (turbidometric method) and release of i-NPY were measured in rat platelet preparations stimulated by a thromboxane agonist (U44069), collagen, thrombin and ADP. All agents induced dose-dependent aggregation, although ADP and U44069 were weak agonists in citrated platelet rich plasma, ADP only inducing primary aggregation. Total i-NPY content in platelet rich plasma of Sprague-Dawley rats was 32.1±3.8 pmol/ml; more than 20 pmol/ml was released into supernatant of aggregating platelets stimulated with high dose thrombin or collagen during secondary, irreversible aggregation. Although U44069 and ADP both stimulated i-NPY release in a dose-dependent manner, release was substantially lower than with the other agonists. We conclude that i-NPY release from rat platelets is associated mainly with secondary, irreversible aggregation, and can be produced by a variety of platelet stimulating agents as part of the platelet release reaction. The present study and other recent studies demonstrate wide variability in platelet i-NPY content between and within species. Investigation of the genetic regulation of i-NPY content in platelets could offer new insights into the relationship between NPY and pathophysiology of the immune and cardiovascular systems.

Platelet Aggregation and Adhesion during Dietary Copper Deficiency in Rats

1996 ◽  
Vol 75 (04) ◽  
pp. 630-634 ◽  
Author(s):  
David G Lominadze ◽  
Jack T Saari ◽  
Frederick N Miller ◽  
James L Catalfamo ◽  
David E Justus ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Platelet Aggregation ◽  
Platelet Adhesion ◽  
Copper Deficiency ◽  
Platelet Rich Plasma ◽  
Sprague Dawley ◽  
Dietary Copper ◽  
Sprague Dawley Rats ◽  
Von Willebrand

SummaryThe role of dietary copper deficiency in platelet-to-endothelial cell adhesion and in platelet-to-platelet aggregation was studied in vitro. Platelets were obtained from male, weanling Sprague-Dawley rats fed purified diets which were either copper-adequate (CuA, 6.3 μg copper/g of diet) or copper-deficient (CuD, 0.3 μg copper/g of diet) for 4 weeks. The platelet adhesion study was performed by adding CuA or CuD platelets either suspended in homologous plasma or in Tyrode buffer salt solution (TBSS) to cultured rat endothelial cells. After a one hour incubation at 37° C non-adhered platelets were removed and counted in a microcytometer. Platelet aggregation in platelet rich plasma (PRP) samples was induced by adding ADP (2 × 10−4 M) and measured in a turbidometric platelet aggregometer. The content of von Willebrand factor (vWF) in platelets and in plasma and the content of fibrinogen in platelets was determined. Platelet adhesion to rat endothelial cells was significantly lower for platelets from CuD rats than for platelets from CuA rats. ADP induced platelet aggregation from CuD rats was significantly higher than platelet aggregation from CuA rats. The content of vWF in platelets and in plasma from CuD rats was significantly lower than in platelets and plasma from CuA rats. However, the amount of fibrinogen in platelets from CuD rats was about 4-fold higher than that in platelets from CuA rats while the plasma fibrinogen was lower in CuD rats than in CuA rats. These studies illustrate that copper deficiency diminishes platelet adhesion to endothelial cells but increases platelet aggregability. The results suggest that these physiological alterations may be the result of decreased platelet vWF and increased platelet fibrinogen during dietary copper deficiency.

Sign in / Sign up

Export Citation Format

Share Document