scholarly journals Construction and Identification of Recombinant Virus BHV-1 Expression of VP60 Gene of RHDV

2017 ◽  
Vol 05 (02) ◽  
pp. 11-18
Author(s):  
冬伟 原
Keyword(s):  
Recombinant Virus ◽  
Vp60 Gene

scholarly journals Microbial metagenomic approach uncovers the first rabbit haemorrhagic disease virus genome in Sub-Saharan Africa

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anise N. Happi ◽  
Olusola A. Ogunsanya ◽  
Judith U. Oguzie ◽  
Paul E. Oluniyi ◽  
Alhaji S. Olono ◽  
...  
Keyword(s):  
Virus Genome ◽  
Sub Saharan Africa ◽  
High Morbidity ◽  
Vp60 Gene ◽  
Band Size ◽  
Rabbit Haemorrhagic Disease ◽  
Sub Saharan ◽  
Domestic Rabbits ◽  
Haemorrhagic Disease ◽  
Pcr Targeting

AbstractRabbit Haemorrhagic Disease (RHD) causes high morbidity and mortality in rabbits and hares. Here, we report the first genomic characterization of lagovirus GI.2 virus in domestic rabbits from sub-Saharan Africa. We used an unbiased microbial metagenomic Next Generation Sequencing (mNGS) approach to diagnose the pathogen causing the suspected outbreak of RHD in Ibadan, Nigeria. The liver, spleen, and lung samples of five rabbits from an outbreak in 2 farms were analyzed. The mNGS revealed one full and two partial RHDV2 genomes on both farms. Phylogenetic analysis showed close clustering with RHDV2 lineages from Europe (98.6% similarity with RHDV2 in the Netherlands, and 99.1 to 100% identity with RHDV2 in Germany), suggesting potential importation. Subsequently, all the samples were confirmed by RHDV virus-specific RT-PCR targeting the VP60 gene with the expected band size of 398 bp for the five rabbits sampled. Our findings highlight the need for increased genomic surveillance of RHDV2 to track its origin, understand its diversity and to inform public health policy in Nigeria, and Sub-Saharan Africa.

scholarly journals Bacmid Expression of Granulovirus Enhancin En3 Accumulates in Cell Soluble Fraction to Potentiate Nucleopolyhedrovirus Infection

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1233
Author(s):  
Adriana Ricarte-Bermejo ◽  
Oihane Simón ◽  
Ana Beatriz Fernández ◽  
Trevor Williams ◽  
Primitivo Caballero
Keyword(s):  
Trichoplusia Ni ◽  
Recombinant Virus ◽  
Spodoptera Exigua ◽  
Insect Cells ◽  
Soluble Fraction ◽  
Autographa Californica ◽  
Insect Midgut ◽  
Baculovirus Infection ◽  
Occlusion Bodies ◽  
Infected Cells

Enhancins are metalloproteinases that facilitate baculovirus infection in the insect midgut. They are more prevalent in granuloviruses (GVs), constituting up to 5% of the proteins of viral occlusion bodies (OBs). In nucleopolyhedroviruses (NPVs), in contrast, they are present in the envelope of the occlusion-derived virions (ODV). In the present study, we constructed a recombinant Autographa californica NPV (AcMNPV) that expressed the Trichoplusia ni GV (TnGV) enhancin 3 (En3), with the aim of increasing the presence of enhancin in the OBs or ODVs. En3 was successfully produced but did not localize to the OBs or the ODVs and accumulated in the soluble fraction of infected cells. As a result, increased OB pathogenicity was observed when OBs were administered in mixtures with the soluble fraction of infected cells. The mixture of OBs and the soluble fraction of Sf9 cells infected with BacPhEn3 recombinant virus was ~3- and ~4.7-fold more pathogenic than BacPh control OBs in the second and fourth instars of Spodoptera exigua, respectively. In contrast, when purified, recombinant BacPhEn3 OBs were as pathogenic as control BacPh OBs. The expression of En3 in the soluble fraction of insect cells may find applications in the development of virus-based insecticides with increased efficacy.

Recovery from autoimmunity of MRL/lpr mice after infection with an interleukin-2/vaccinia recombinant virus

Nature ◽  
1990 ◽  
Vol 346 (6281) ◽  
pp. 271-274 ◽  
Author(s):  
Jose C. Gutierrez-Ramos ◽  
Jose L. Andreu ◽  
Yolanda Revilla ◽  
Eladio Viñuela ◽  
Carlos Martinez-A
Keyword(s):  
Recombinant Virus ◽  
Interleukin 2

scholarly journals Immunoreactivity and trypsin sensitivity of recombinant virus-like particles of foot-and-mouth disease virus

2015 ◽  
Vol 59 (01) ◽  
pp. 84-91 ◽  
Author(s):  
S. H. BASAGOUDANAVAR ◽  
M. HOSAMANI ◽  
R. P. TAMIL ◽  
B. P. SREENIVASA ◽  
B. K. CHANDRASEKHAR ◽  
...  
Keyword(s):  
Disease Virus ◽  
Recombinant Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Virus Like Particles ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Identification of Two Additional Translation Products from the Matrix (M) Gene That Contribute to Vesicular Stomatitis Virus Cytopathology

2002 ◽  
Vol 76 (16) ◽  
pp. 8011-8018 ◽  
Author(s):  
Himangi R. Jayakar ◽  
Michael A. Whitt
Keyword(s):  
Amino Acids ◽  
Vesicular Stomatitis Virus ◽  
Recombinant Virus ◽  
M Protein ◽  
M Gene ◽  
Cell Rounding ◽  
Matrix Gene ◽  
The Matrix ◽  
Infected Cells ◽  
Vesicular Stomatitis

ABSTRACT The matrix (M) protein of vesicular stomatitis virus (VSV) is a multifunctional protein that is responsible for condensation of the ribonucleocapsid core during virus assembly and also plays a critical role in virus budding. The M protein is also responsible for most of the cytopathic effects (CPE) observed in infected cells. VSV CPE include inhibition of host gene expression, disablement of nucleocytoplasmic transport, and disruption of the host cytoskeleton, which results in rounding of infected cells. In this report, we show that the VSV M gene codes for two additional polypeptides, which we have named M2 and M3. These proteins are synthesized from downstream methionines in the same open reading frame as the M protein (which we refer to here as M1) and lack the first 32 (M2) or 50 (M3) amino acids of M1. Infection of cells with a recombinant virus that does not express M2 and M3 (M33,51A) resulted in a delay in cell rounding, but virus yield was not affected. Transient expression of M2 and M3 alone caused cell rounding similar to that with the full-length M1 protein, suggesting that the cell-rounding function of the M protein does not require the N-terminal 50 amino acids. To determine if M2 and M3 were sufficient for VSV-mediated CPE, both M2 and M3 were expressed from a separate cistron in a VSV mutant background that readily establishes persistent infections and that normally lacks CPE. Infection of cells with the recombinant virus that expressed M2 and M3 resulted in cell rounding indistinguishable from that with the wild-type recombinant virus. These results suggest that M2 and M3 are important for cell rounding and may play an important role in viral cytopathogenesis. To our knowledge, this is first report of the multiple coding capacities of a rhabdovirus matrix gene.

scholarly journals Generation of infectious and transmissible virions from a GB virus B full-length consensus clone in tamarins

2001 ◽  
Vol 82 (10) ◽  
pp. 2437-2448 ◽  
Author(s):  
Andrea Sbardellati ◽  
Elisa Scarselli ◽  
Ernst Verschoor ◽  
Amedeo De Tomassi ◽  
Domenico Lazzaro ◽  
...  
Keyword(s):  
Immune Response ◽  
Animal Model ◽  
Recombinant Virus ◽  
Secondary Infection ◽  
Viral Rna ◽  
Hepatic Tissue ◽  
Cdna Clones ◽  
Humoral Immune ◽  
B Genome

The strong similarity between GB virus B (GBV-B) and hepatitis C virus (HCV) makes tamarins infected by GBV-B an acceptable surrogate animal model for HCV infection. Even more attractive, for drug discovery purposes, is the idea of constructing chimeric viruses by inserting HCV genes of interest into a GBV-B genome frame. To accomplish this, infectious cDNA clones of both viruses must be available. The characterization of several HCV molecular clones capable of infecting chimpanzees has been published, whereas only one infectious GBV-B clone inducing hepatitis in tamarins has been reported so far. Here we describe the infection of tamarins by intrahepatic injection of RNA transcribed from a genomic GBV-B clone (FL-3) and transmission of the disease from infected to naive tamarins via serum inoculation. The disease resulting from both direct and secondary infection was characterized for viral RNA titre and hepatitis parameters as well as for viral RNA distribution in the hepatic tissue. Host humoral immune response to GBV-B antigens was also monitored. The progression of the disease was compared to that induced by intravenous injection of different amounts of the non-recombinant virus.

Design of recombinant virus vaccines for Lassa fever

1988 ◽  
Vol 11 ◽  
pp. 67
Author(s):  
David D. Auperin ◽  
Hilary G. Morrison ◽  
Joseph J. Esposito ◽  
James V. Lange ◽  
Susan P. Fisher-Hoch ◽  
...  
Keyword(s):  
Recombinant Virus ◽  
Lassa Fever
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