Hybrid Purity Identification of Liyouza No. 3 in Bassica napus

2019 ◽  
Vol 08 (03) ◽  
pp. 240-246
Author(s):  
时艺 周
Keyword(s):  
Hybrid Purity

Authentication of Hybrid Purity in Eggplant (Solanum melongenaL.) Through RAPD Analysis

2015 ◽  
Vol 28 (1) ◽  
pp. 109
Author(s):  
Rajshree Shukla ◽  
Nandan Mehta ◽  
Ritu R Saxena ◽  
S B Verulkar
Keyword(s):  
Rapd Analysis ◽  
Hybrid Purity

Advances of melon hybrid purity test

2018 ◽  
pp. 115-118
Author(s):  
C. Li ◽  
Y.P. Sun ◽  
Y. Yang ◽  
C. Xu ◽  
X.F. Liao
Keyword(s):  
Hybrid Purity ◽  
Purity Test

scholarly journals New Hypervariable SSR Markers for Diversity Analysis, Hybrid Purity Testing and Trait Mapping in Pigeonpea [Cajanus cajan (L.) Millspaugh]

2017 ◽  
Vol 8 ◽  
Author(s):  
Abhishek Bohra ◽  
Rintu Jha ◽  
Gaurav Pandey ◽  
Prakash G. Patil ◽  
Rachit K. Saxena ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Cajanus Cajan ◽  
Diversity Analysis ◽  
Trait Mapping ◽  
Purity Testing ◽  
Hybrid Purity

scholarly journals Feasibility of terahertz spectroscopy for hybrid purity verification of rice seeds

2018 ◽  
Vol 11 (5) ◽  
pp. 65-69 ◽  
Author(s):  
Yaling Yang ◽  
◽  
Shengling Zhou ◽  
Jie Song ◽  
Jie Huang ◽  
...  
Keyword(s):  
Terahertz Spectroscopy ◽  
Hybrid Purity ◽  
Rice Seeds

Cotton Hybrid Purity Tested by SSR Markers and Its Correlation with Pheno-type Identification in Field

2012 ◽  
Vol 37 (12) ◽  
pp. 2299-2305 ◽  
Author(s):  
Meng KUANG ◽  
Wei-Hua YANG ◽  
Yu-Cui ZHANG ◽  
Hong-Xia XU ◽  
Yan-Qin WANG ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Hybrid Purity ◽  
Cotton Hybrid ◽  
Pheno Type

scholarly journals Hybrid Purity Testing of Brassica rapa Using SSR Marker Technology

HortScience ◽  
2017 ◽  
Vol 52 (10) ◽  
pp. 1342-1348 ◽  
Author(s):  
Li Li ◽  
Ling Liu ◽  
Deshuang Zhang ◽  
Ping Wu ◽  
Fenglan Zhang ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Detection System ◽  
Rapid Identification ◽  
Genotype Frequencies ◽  
Purity Testing ◽  
Significant Difference ◽  
Hybrid Purity ◽  
Parental Inbred ◽  
Simple Sequence

Thirteen Chinese cabbage (Brassica rapa) hybrid cultivars and 26 parental inbred lines were used as experimental materials to screen for primers producing hybrid and parental complementary bands and for primers with high polymorphism information contents and low genotype frequencies. A total of 18 pairs of core primers were designed to identify the purity of Chinese cabbage. There was no significant difference in the purity percentage measured between different loci of the same strain. The fingerprint obtained by the amplification of each locus could be used to identify purity to obtain an authentic purity percentage. Curve mapping and significance analyses were conducted using the purity percentage of eight different seed samples and confirmed a sampling seed number of 96. The results of the purity test were verified by comparison with the grow-out test (GOT) using molecular markers. In conclusion, the simple sequence repeat (SSR) detection system could be used for the rapid identification of the purity of the tested Chinese cabbage hybrids.

scholarly journals Application of SSR Markers for Purity Testing of Hybrid Wheat (Triticum aestivum L.)

2020 ◽  
pp. 31-40
Author(s):  
Akanksha Tiwari ◽  
D. K. Mishra
Keyword(s):  
Ssr Markers ◽  
Research Programme ◽  
Polymorphic Marker ◽  
Accurate Method ◽  
Triticum Aestivum L ◽  
Full Potential ◽  
Purity Analysis ◽  
Hybrid Purity ◽  
Parental Lines ◽  
Wheat Hybrids

Exploitation of the full potential of any hybrid requires the possessing of genetically high-purity seeds. In order to avoid reduction in yield caused by using low purity seeds, development of a simple, rapid, and accurate method for hybrid purity assessment is of great essence and significance. For the identification of true hybrids, SSR markers provides authentic information that will help to the further progress of any research programme. In this study, total of 40 wheat hybrids and 14 parental lines were taken for the hybrid purity analysis with 20 SSR markers. These markers were used to find out the codominant loci in the hybrid and single dominant loci in parents. Out of 20 SSR markers, only 8 markers viz., Xwmc617, Xwmc457, Xwmc48, Xgwm153, Xbarc61, Xgwm273, Xbarc268 and Xgwm274 showed the polymorphic dominant loci in the parents and co-dominant loci midway between these parents. Therefore these SSR markers were used to confirm the forty hybrids. The range of allele size produced by SSR marker on the parental lines were 120 bp to 300 bp. The highest allel size (300 bp) was produced by marker Xbarc 268 whereas, the minimum allel size (120 bp) was produced by marker Xgwm 273. All the fourty hybrids showed similar banding pattern as parental lines, this proved the purity of hybrids in all these cross combinations. The confirmation of hybrid purity indicated that a single polymorphic marker was sufficient for detection of contaminations of these hybrids from their parents.

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