scholarly journals Identification and Control of Neurospora crassa Causing High Rate of Pollution in Tissue Culture of Rubber Tree

2017 ◽  
Vol 06 (06) ◽  
pp. 385-392
Author(s):  
婷 郭
Keyword(s):  
Tissue Culture ◽  
Neurospora Crassa ◽  
Rubber Tree ◽  
High Rate ◽  
And Control

scholarly journals Osteoblastic Response to the Defective Matrix in the Osteogenesis Imperfecta Murine (oim) Mouse

Endocrinology ◽  
2002 ◽  
Vol 143 (5) ◽  
pp. 1594-1601 ◽  
Author(s):  
I. Kalajzic ◽  
J. Terzic ◽  
Z. Rumboldt ◽  
K. Mack ◽  
A. Naprta ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Osteogenesis Imperfecta ◽  
Bone Matrix ◽  
High Rate ◽  
Transferase Activity ◽  
Osteoblast Activity ◽  
Chloramphenicol Acetyl Transferase Activity ◽  
High Bone ◽  
Normal Quantity ◽  
And Control

Abstract This work examines the cellular pathophysiology associated with the weakened bone matrix found in a murine model of osteogenesis imperfecta murine (oim). Histomorphometric analysis of oim/oim bone showed significantly diminished bone mass, and the osteoblast and osteoclast histomorphometric parameters were increased in the oim/oim mice, compared with wild-type (+/+) mice. To assess osteoblast activity, a rat Col1a1 promoter linked to the chloramphenicol acetyltransferase reporter transgene was bred into the oim model. At 8 d and 1 month of age, no difference in transgene activity between oim and control mice was observed. However, at 3 months of age, chloramphenicol acetyl transferase activity was elevated in oim/oim;Tg/Tg, compared with +/+;Tg/Tg and oim/+;Tg/Tg. High levels of urinary pyridinoline crosslinks in the oim/oim;Tg/Tg mice were present at all ages, reflecting continuing high bone resorption. Our data portray a state of ineffective osteogenesis in which the mutant mouse never accumulates a normal quantity of bone matrix. However, it is only after the completion of the rapid growth phase that the high activity of the oim/oim osteoblast can compensate for the high rate of bone resorption. This relationship between bone formation and resorption may explain why the severity of osteogenesis imperfecta decreases after puberty is completed. The ability to quantify high bone turnover and advantages of using a transgene that reflects osteoblast lineage activity make this a useful model for studying interventions designed to improve the bone strength in osteogenesis imperfecta.

scholarly journals The behavioral effects of antibiotic treatment on the snailBiomphalaria glabrata

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e4171 ◽  
Author(s):  
Euan R.O. Allan ◽  
Michael S. Blouin
Keyword(s):  
Tissue Culture ◽  
Intermediate Host ◽  
Antibiotic Treatment ◽  
New World ◽  
Biomphalaria Glabrata ◽  
Behavioral Effects ◽  
Neglected Tropical Disease ◽  
Intermediate Hosts ◽  
And Control ◽  
Main Model

Schistosomiasis is a detrimental neglected tropical disease that is transmitted by Planorbid snails. Understanding the transmission and control of this disease requires an extensive understanding of these intermediate hosts, which is only achieved by the effective rearing and study of species such asBiomphalaria glabrata. This species is the intermediate host forSchistosoma mansoniin the New World, and is also the main model for studying schistosomes in mollusks. Antibiotics are used routinely inB. glabratatissue culture, and occasionally on live snails. Here we show that standard doses of three common antibiotics (penicillin, streptomycin and gentamicin) drastically diminish the activity of healthyB. glabrata, but that treated snails recover rapidly when placed in fresh water. Ampicillin treated snails did not show altered activity. We suggest that researchers keep these apparent toxicities in mind if a need for antibiotic treatment of live Planorbid snails arises.

scholarly journals Proteomic Differences in the Hippocampus and Cortex of Epilepsy Brain Tissue

2020 ◽  
Author(s):  
Geoffrey Pires ◽  
Dominique Leitner ◽  
Eleanor Drummond ◽  
Evgeny Kanshin ◽  
Shruti Nayak ◽  
...  
Keyword(s):  
Dentate Gyrus ◽  
Frontal Cortex ◽  
G Protein ◽  
Molecular Mechanisms ◽  
High Rate ◽  
Label Free ◽  
Protein Subunit ◽  
Unexpected Death ◽  
Hippocampal Ca1 ◽  
And Control

AbstractEpilepsy is a common neurological disorder affecting over 70 million people worldwide, with a high rate of pharmaco-resistance, diverse comorbidities including progressive cognitive and behavioral disorders, and increased mortality from direct (e.g., Sudden Unexpected Death in Epilepsy [SUDEP], accidents, drowning) or indirect effects of seizures and therapies. Extensive research with animal models and human studies provides limited insights into the mechanisms underlying seizures and epileptogenesis, and these have not translated into significant reductions in pharmaco-resistance, morbidities or mortality. To help define changes in molecular signaling networks associated with epilepsy, we examined the proteome of brain samples from epilepsy and control cases. Label-free quantitative mass spectrometry (MS) was performed on the hippocampal CA1-3 region, frontal cortex, and dentate gyrus microdissected from epilepsy and control cases (n=14/group). Epilepsy cases had significant differences in the expression of 777 proteins in the hippocampal CA1-3 region, 296 proteins in the frontal cortex, and 49 proteins in the dentate gyrus in comparison to control cases. Network analysis showed that proteins involved in protein synthesis, mitochondrial function, G-protein signaling, and synaptic plasticity were particularly altered in epilepsy. While protein differences were most pronounced in the hippocampus, similar changes were observed in other brain regions indicating broad proteomic abnormalities in epilepsy. Among the most significantly altered proteins, G-protein Subunit Beta 1 (GNB1) was one of the most significantly decreased proteins in epilepsy in all regions studied, highlighting the importance of G-protein subunit signaling and G-protein–coupled receptors (GPCRs) in epilepsy. Our results provide insights into the molecular mechanisms underlying epilepsy, which may allow for novel targeted therapeutic strategies.

Discrepancies in carcinoembryonic antigen measurements: survey and control values vs values for patients.

1987 ◽  
Vol 33 (4) ◽  
pp. 563-566 ◽  
Author(s):  
G G Klee ◽  
L A Dodge ◽  
G Reynoso
Keyword(s):  
Tissue Culture ◽  
Carcinoembryonic Antigen ◽  
Enzyme Immunoassay ◽  
Colon Carcinoma ◽  
Proficiency Testing ◽  
Analytical Methods ◽  
Test Results ◽  
And Control ◽  
The Relationship ◽  
Metastatic Colon Carcinoma

Abstract We analyzed the carcinoembryonic antigen (CEA) test results reported in the College of American Pathologists' (CAP) surveys to determine the relationship between the source of CEA used to manufacture the survey specimens and the discrepancies among analytical methods. With the 1983 survey specimens, which were prepared from metastatic colon carcinoma, laboratories using Roche RIA with Clinetics columns reported results that were only one-half the values reported by laboratories using the Abbott polyclonal enzyme immunoassay. With the 1984 specimens, prepared from a different metastatic colon carcinoma, and the 1985 specimens, prepared from a tissue-culture source of CEA, the Roche results were about one-sixth as large as the Abbott results. These differences are larger than the reported assay differences for patients' specimens. In addition, twofold proportional differences were found when survey and control specimens were tested with different lots of Abbott polyclonal reagent, whereas only random differences were found with 102 patients' specimens. Evidently, assay systems perform differently with proficiency-testing and control specimens than with patients' specimens.

CULTIVATION OF POLIOMYELITIS VIRUS IN TISSUE CULTURE

1952 ◽  
Vol 30 (3) ◽  
pp. 231-245 ◽  
Author(s):  
Joan C. Thicke ◽  
Darline Duncan ◽  
William Wood ◽  
A. E. Franklin ◽  
A. J. Rhodes
Keyword(s):  
Tissue Culture ◽  
Cell Morphology ◽  
Synthetic Medium ◽  
Embryonic Brain ◽  
Human Embryonic Kidney ◽  
Virus Growth ◽  
Nutritive Medium ◽  
Poliomyelitis Virus ◽  
Cytopathogenic Effect ◽  
And Control

This paper presents observations on the growth of Lansing poliomyelitis virus in fluid cultures of various human embryonic and adult tissues. The evidence suggests that viral multiplication has occurred in cultures of monkey testis, human embryonic kidney, and mixtures of brain and cord. Satisfactory virus growth has been obtained particularly in cultures containing human embryonic brain and cord. Virus is present in tissue culture fluids in which the original inoculum has been diluted 10−33.3 by subcultivation. Preliminary observations suggest that a synthetic medium (Mixture 199) devised by Morgan, Morton, and Parker is superior to Hanks–Simms solution as a nutritive medium in such cultures. The cytopathogenic effect of the virus, as revealed by pH determinations and cell morphology, has been noted, although a characteristic pH differential between virus infected and control flasks was not commonly observed. Attempts to grow the virus on a larger scale in Kolle flasks are described.

scholarly journals An Evaluation of Dazomet Incorporation Methods on Soilborne Organisms and Pine Seedling Production in Southern Nurseries

2003 ◽  
Vol 27 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Stephen W. Fraedrich ◽  
L. David Dwinell
Keyword(s):  
Morphological Characteristics ◽  
High Rate ◽  
Parasitic Nematodes ◽  
Seedling Production ◽  
Seedling Density ◽  
The North ◽  
Nursery Plant ◽  
Shoot Weight ◽  
And Control

Abstract The use of dazomet as a fall and spring fumigant for pine seedling production and control of soilborne pests was evaluated at two southern nurseries. Dazomet was applied at low (280–325 kg/ha) and high (493–560 kg/ha) rates and incorporated with a rototiller or spading machine. Comparisons were made with methyl bromide/chloropicrin (MBC) fumigation and nonfumigated control treatments. Dazomet incorporation method had no effect on seedling density at either nursery, and often did not affect seedling morphological characteristics. At the Georgia (GA) nursery, seedling density and morphological characteristics did not differ among fumigant treatments except in the spring study area where shoot weight was greater in the MBC treatment than the dazomet or nonfumigated control treatments. In the fall study area at the North Carolina (NC) nursery, seedling density was greater in the high-rate dazomet treatment than the nonfumigated control. Seedlings were generally larger in MBC and dazomet treatments than the control. Seedling density and morphological characteristics did not differ among fumigation treatments in the spring study area. Fumigation with MBC or dazomet generally reduced the percentage of roots withPythium andFusarium spp. compared to controls at the GA nursery and the fall fumigation area in the NC nursery. Plant parasitic nematodes were found infrequently at both nurseries and did not differ among treatments. Nutsedge (Cyperus spp.) was the major problem at the GA nursery and was effectively controlled only with MBC. Compared to the MBC treatment, the abundance of soilborne fungi and the association of certain types ofTrichoderma with roots was often lower in the dazomet treatments. The importance of these differences for long term seedling production and management of soilborne diseases is not known at this time. South. J. Appl. For. 27(1):41–51.

scholarly journals Principles of Formation of a Vehicle’s Remote Diagnostics

2019 ◽  
Vol 302 ◽  
pp. 01019
Author(s):  
Łukasz Muślewski ◽  
Roman Zinko ◽  
Oleg Polishchuk
Keyword(s):  
Remote Access ◽  
High Rate ◽  
Control Parameters ◽  
Diagnostics And Control ◽  
Preventive Control ◽  
Serial Production ◽  
Remote Diagnostics ◽  
Intellectual System ◽  
Access To Data ◽  
And Control

Complex of remote diagnostics and control of an automobile’s nodes enables to remotely control the state of vehicle’s nodes and diagnose malfunctions; control ecological norms and compliance with traffic regulations. Its implementation will allow diagnostics of intermittent malfunctions due to application of the proposed methods of remote diagnostics of an automobile’s nodes as well as to reduce terms of diagnostics significantly due to application of intellectual system of malfunctions detection and due to possibility to control parameters in real time mode. Implementation of the complex will cut unforeseen idle time of vehicles due to preventive control of the tendencies in nodes parameters changes, and due to executing modern repair or replacement of nodes and units. The complex grants safe local and remote access to data and reports by using WebPortal and VPN technologies with possibility to appoint personal right to every user. Due to application of the elaborated methods and the complex that implements it, one can achieve high rate of economic efficiency due to collection of statistic information under conditions of actual exploitation, diagnostics of emerging malfunctions of various difficulty degrees, and making necessary corrections in the structure of an automobile’s nodes prior to serial production launch.

scholarly journals Endogenous Bacterial Contamination of Plant Tissue Culture Materials: Identification and Control Strategy

2018 ◽  
Vol 28 (1) ◽  
pp. 99-108 ◽  
Author(s):  
Mohammad Ali ◽  
Shefali Boonerjee ◽  
Mohammad Nurul Islam ◽  
Mihir Lal Saha ◽  
M Imdadul Hoque ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Bacterial Contamination ◽  
Antibiotic Sensitivity ◽  
Sensitivity Test ◽  
Bacterial Isolates ◽  
Culture And Sensitivity ◽  
And Control

The endogenous bacterial contamination of plant tissue culture materials and their possible control was studied. Nine bacterial isolates were isolated from the contaminated tissue culture materials viz. potato and tea. On the basis of morphology and biochemical characters of nine isolates, seven were identified as Gram positive belonging to Bacillus alcalophilus, B. circulans, B. infantis, B. lentus, B. schlegelii, B. pumilus and B. subtilis. Remaining two were Gram negative and identified as Enterobacter cloacae sub. sp. dissolvens and Pantoea agglomerans. Molecular analysis was conducted on the basis of 16S rDNA sequence to confirm three isolates. Culture and sensitivity test was carried out to screen out the antibiotic sensitivity where streptomycin (S-10), polymyxin (PB-300) and gentamicin (CN-120) antibiotics were found to be effective against all bacterial isolates. The culture and sensitivity test reflected the feasibility to control or eliminate the contaminant bacteria during in vitro culture of plant which is very much required in the commercial tissue culture production.Plant Tissue Cult. & Biotech. 28(1): 99-108, 2018 (June)

scholarly journals Roasted coffee wastes as a substrate for Escherichia coli to grow and produce hydrogen

2020 ◽  
Vol 367 (11) ◽  
Author(s):  
Hripsime Petrosyan ◽  
Liana Vanyan ◽  
Satenik Mirzoyan ◽  
Armen Trchounian ◽  
Karen Trchounian
Keyword(s):  
Escherichia Coli ◽  
H2 Production ◽  
High Rate ◽  
Main Role ◽  
Wild Type ◽  
Roasted Coffee ◽  
Energy Needs ◽  
Genetic Modifications ◽  
Coffee Grounds ◽  
And Control

ABSTRACT After brewing roasted coffee, spent coffee grounds (SCGs) are generated being one of the daily wastes emerging in dominant countries with high rate and big quantity. Escherichia coli BW25113 wild-type strain, mutants with defects in hydrogen (H2)-producing/oxidizing four hydrogenases (Hyd) (ΔhyaB ΔhybC, ΔhycE, ΔhyfG) and septuple mutant (ΔhyaB ΔhybC ΔhycA ΔfdoG ΔldhA ΔfrdC ΔaceE) were investigated by measuring change of external pH, bacterial growth and H2 production during the utilization of SCG hydrolysate. In wild type, H2 was produced with rate of 1.28 mL H2 (g sugar)−1 h−1 yielding 30.7 mL H2 (g sugar)−1 or 2.75 L (kg SCG)−1 during 24 h. In septuple mutant, H2 production yield was 72 mL H2 (g sugar)−1 with rate of 3 mL H2 (g sugar)−1 h−1. H2 generation was absent in hycE single mutant showing the main role of Hyd-3 in H2 production. During utilization of SCG wild type, specific growth rate was 0.72 ± 0.01 h−1 with biomass yield of 0.3 g L−1. Genetic modifications and control of external parameters during growth could lead to prolonged and enhanced microbiological H2 production by organic wastes, which will aid more efficiently global sustainable energy needs resulting in diversification of mobile and fixed energy sources.

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