scholarly journals Application of SRAP Molecular Techniques in Plant Genome Research

2013 ◽  
Vol 02 (03) ◽  
pp. 87-91
Author(s):  
彦锦 李
Keyword(s):  
Molecular Techniques ◽  
Plant Genome ◽  
Genome Research

scholarly journals Upstream – News in Genomics

2001 ◽  
Vol 2 (6) ◽  
pp. 355-358
Keyword(s):  
Experimental Study ◽  
National Science Foundation ◽  
Mouse Genome ◽  
Genome Project ◽  
Plant Genome ◽  
Genome Research ◽  
Human Genes ◽  
The Us ◽  
National Science ◽  
Mouse Genome Project

Since our last issue, several important genomes have been completely or ‘almost completely’ sequenced. The debate over the number of human genes has flared up once more, with one computational and one experimental study into the annotation of the human genome. The mouse genome project has a clone fingerprint map to aid their sequencing effort. The SAGE technique has been applied toDrosophilaand the US National Science Foundation announced increased spending on plant genome research.

Evidence of Rubus Yellow Net Virus Integration into the Red Raspberry Genome

2020 ◽  
Vol 160 (6) ◽  
pp. 329-334
Author(s):  
Alfredo Diaz-Lara ◽  
Nola J. Mosier ◽  
Kristian Stevens ◽  
Karen E. Keller ◽  
Robert R. Martin
Keyword(s):  
High Throughput Sequencing ◽  
Rolling Circle Amplification ◽  
Standard Test ◽  
Molecular Techniques ◽  
Test Method ◽  
Plant Genome ◽  
Red Raspberry ◽  
Rolling Circle ◽  
Certification Programs ◽  
Dnase Treatment

Rubus yellow net virus (RYNV) infects Rubus spp., causing a severe decline when present in mixed infections with other viruses. RYNV belongs to the family Caulimoviridae, also known as plant pararetroviruses, which can exist as episomal or integrated elements (endogenous). Most of integrated pararetroviruses are noninfectious; however, a few cases have been reported where they excised from the plant genome and formed infectious particles. Graft transmission onto indicator plants R. occidentalis “Munger” has been the standard test method for RYNV detection in certification programs. Previously, it was noticed that some RYNV PCR-positive plants did not induce symptoms on “Munger”, suggesting an integration event. In this study, bio-indexing and different molecular techniques were employed to differentiate between integrated and episomal RYNV sequences. Reverse transcription-PCR using RYNV-specific oligonucleotides after DNase treatment generated positive results for the virus in graft transmissible isolates (episomal) only. To confirm these results, rolling circle amplification on DNA preparations from the same samples resulted in amplicons identified as RYNV only from plants with graft transmissible RYNV. High-throughput sequencing was used to identify the RYNV-like sequences present in the host DNA. These results indicate the integration of RYNV into the red raspberry genome and highlight the necessity to recognize this phenomenon (integration) in future Rubus quarantine and certification programs.

scholarly journals MIPS PlantsDB: a database framework for comparative plant genome research

2012 ◽  
Vol 41 (D1) ◽  
pp. D1144-D1151 ◽  
Author(s):  
Thomas Nussbaumer ◽  
Mihaela M. Martis ◽  
Stephan K. Roessner ◽  
Matthias Pfeifer ◽  
Kai C. Bader ◽  
...  
Keyword(s):  
Plant Genome ◽  
Genome Research

scholarly journals MIPSPlantsDB--plant database resource for integrative and comparative plant genome research

2007 ◽  
Vol 35 (Database) ◽  
pp. D834-D840 ◽  
Author(s):  
M. Spannagl ◽  
O. Noubibou ◽  
D. Haase ◽  
L. Yang ◽  
H. Gundlach ◽  
...  
Keyword(s):  
Plant Genome ◽  
Genome Research

scholarly journals Plant Genome Horizons: Michael Bennett's Contribution to Genome Research

2008 ◽  
Vol 101 (6) ◽  
pp. 737-746 ◽  
Author(s):  
I. J. Leitch ◽  
M. F. Fay
Keyword(s):  
Plant Genome ◽  
Genome Research

scholarly journals Plant Genome Research Outreach Portal. A Gateway to Plant Genome Research “Outreach” Programs and Activities

2004 ◽  
Vol 134 (3) ◽  
pp. 889-889 ◽  
Author(s):  
Sanford B. Baran ◽  
Carolyn J. Lawrence ◽  
Volker Brendel
Keyword(s):  
Plant Genome ◽  
Genome Research ◽  
Outreach Programs
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