scholarly journals Comparative Study of the Proliferative Activity of Serous- and Mucous-type Acinar Cells in Developing Mongolian Gerbil Mixed Salivary Glands

2002 ◽  
Vol 35 (4) ◽  
pp. 343-345 ◽  
Author(s):  
Toshihiro Miyazaki ◽  
Yasuhisa Inoue ◽  
Kunio Takano
Keyword(s):  
Comparative Study ◽  
Salivary Glands ◽  
Mongolian Gerbil ◽  
Proliferative Activity ◽  
Acinar Cells

scholarly journals Neurofibromin expression by normal salivary glands

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Eloá Borges Luna ◽  
Pâmella Pinho Montovani ◽  
Rafaela Elvira Rozza-de-Menezes ◽  
Karin Soares Cunha
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Acinar Cells ◽  
Staining Intensity ◽  
Neurofibromatosis 1 ◽  
Tissue Type ◽  
Sublingual Gland ◽  
Minor Salivary Glands ◽  
Expression Levels ◽  
Ductal Cells

AbstractIntroductionNeurofibromin, a protein encoded by theNF1gene, is mutated in neurofibromatosis 1, one of the most common genetic diseases. Oral manifestations are common and a high prevalence of hyposalivation was recently described in individuals with neurofibromatosis 1. Although neurofibromin is ubiquitously expressed, its expression levels vary depending on the tissue type and developmental stage of the organism. The role of neurofibromin in the development, morphology, and physiology of salivary glands is unknown and a detailed expression of neurofibromin in human normal salivary glands has never been investigated.AimTo investigate the expression levels and distribution of neurofibromin in acinar and ductal cells of major and minor salivary glands of adult individuals without NF1.Material and methodTen samples of morphologically normal major and minor salivary glands (three samples of each gland: parotid, submandibular and minor salivary; and one sample of sublingual gland) from individuals without neurofibromatosis 1 were selected to assess neurofibromin expression through immunohistochemistry. Immunoquantification was performed by a digital method.ResultsNeurofibromin was expressed in the cytoplasm of both serous and mucous acinar cells, as well as in ducts from all the samples of salivary glands. Staining intensity varied from mild to strong depending on the type of salivary gland and region (acini or ducts). Ducts had higher neurofibromin expression than acinar cells (p = 0.003). There was no statistical association between the expression of neurofibromin and the type of the salivary gland, considering acini (p = 0.09) or ducts (p = 0.50) of the four salivary glands (parotid, submandibular, minor salivary, and sublingual gland). Similar results were obtained comparing the acini (p = 0.35) and ducts (p = 0.50) of minor and major salivary glands. Besides, there was no correlation between the expression of neurofibromin and age (p = 0.08), and sex (p = 0.79) of the individuals, considering simultaneously the neurofibromin levels of acini and duct (n = 34).ConclusionNeurofibromin is expressed in the cytoplasm of serous and mucous acinar cells, and ductal cells of salivary glands, suggesting that this protein is important for salivary gland function.

scholarly journals Autoradiographic localization of dihydrotestosterone binding in the major salivary glands and other androgen-responsive organs of the mouse.

1987 ◽  
Vol 35 (10) ◽  
pp. 1053-1058 ◽  
Author(s):  
J I Morrell ◽  
E W Gresik ◽  
T Barka
Keyword(s):  
Growth Factor ◽  
Salivary Glands ◽  
Submandibular Gland ◽  
Cellular Localization ◽  
Incidental Finding ◽  
Acinar Cells ◽  
Unexpected Finding ◽  
Cervical Lymph Nodes ◽  
Major Salivary Glands ◽  
Duct Cells

Mouse submandibular glands show an androgen-dependent sexual dimorphism, reflected in higher concentrations in males than in females of bioactive peptides, such as epidermal growth factor (EGF), nerve growth factor, and renin in the cells of the granular convoluted tubules (GCT). Biochemical studies have demonstrated androgen receptors in submandibular gland and other androgen-responsive organs in mouse. We have determined the cellular localization of these receptors using steroid autoradiography. Fifteen adult gonadectomized male mice were injected intravenously with 0.13 microgram or 0.26 microgram [3H]-dihydrotestosterone (SA 135 Ci/mM); some animals were pre-treated with cyclocytidine to stimulate secretion by GCT cells. Animals were killed 15 min, 1, 2, or 3 hr after isotope injection. Steroid autoradiographs were prepared, and some were stained immunocytochemically for EGF. Of the different cell types of submandibular gland, the acinar cells most frequently and intensely concentrated [3H]-DHT; GCT cells also concentrated the hormone, as did a small number of striated duct cells. In the other major salivary glands, the only cells that concentrated the androgen were interlobular striated duct cells in sublingual gland. In prostate, anterior pituitary, and brain a large number of cells concentrated androgen, as has been previously reported. Androgen binding by the GCT cells was a predictable finding, since androgen-induced alterations in composition and form of these cells are well documented. The intense androgen concentration by the acinar cells was an unexpected finding and suggests a hitherto unknown androgen regulation of these cells. An incidental finding was intense concentration of [3H]-DHT in the nuclei of the endothelial cells of the post-capillary venules of the cervical lymph nodes.

PLAG1 enhances the stemness profiles of acinar cells in normal human salivary glands in a cell type-specific manner

2020 ◽  
Vol 62 (1) ◽  
pp. 99-106 ◽  
Author(s):  
Yuriko Goto ◽  
Miho Ibi ◽  
Hirotaka Sato ◽  
Junichi Tanaka ◽  
Rika Yasuhara ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Acinar Cells ◽  
Cell Type ◽  
Specific Manner ◽  
A Cell ◽  
Normal Human ◽  
Cell Type Specific

Comparative Study of CD8+ Lymphocytes in Salivary Glands And Liver of Patients With Chronic Hepatitis C

2014 ◽  
Vol 117 (2) ◽  
pp. e202
Author(s):  
KARLA RACHEL OLIVEIRA E SILVA ◽  
PATRÍCIA CARLOS CALDEIRA ◽  
PAULA VIEIRA TEIXEIRA VIDIGAL ◽  
MARIA AUXILIADORA VIEIRA DO CARMO
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Comparative Study ◽  
Salivary Glands ◽  
Chronic Hepatitis C

California encephalitis virus proliferation in Yukon mosquitoes incubated at low temperatures

1976 ◽  
Vol 22 (8) ◽  
pp. 1128-1136 ◽  
Author(s):  
D. M. McLean ◽  
P. N. Grass ◽  
B. D. Judd ◽  
K. S. K. Wong
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Salivary Glands ◽  
Low Temperatures ◽  
Acinar Cells ◽  
Yukon Territory ◽  
Immunoperoxidase Staining ◽  
Snowshoe Hare ◽  
Intrathoracic Inoculation ◽  
Aedes Communis

Replication of a subarctic Bunyavirus, California encephalitis (snowshoe hare subtype), was detected in salivary glands and thoraces of wild-caught Aedes communis mosquitoes from the Yukon Territory, after intrathoracic inoculation with 0.1 to 100 mouse LD50 virus, and incubation for 7 to 21 days throughout their viable temperature range of 0 to 23 °C. Immunoperoxidase staining confirmed that viral replication occurred in the cytoplasm of acinar cells of salivary glands, both by light microscopy and electron microscopy. Replication of another subarctic Bunyavirus. Northway, and a subtropical Flavivirus, Murray Valley encephalitis, was also demonstrated by infectivity titrations and immunoperoxidase reactions in salivary glands of A. communis incubated at 0, 13, and 23 °C for 7 to 21 days.

Simple mucin-type carbohydrate antigens in major salivary glands.

1994 ◽  
Vol 42 (9) ◽  
pp. 1251-1259 ◽  
Author(s):  
M H Therkildsen ◽  
U Mandel ◽  
J Thorn ◽  
M Christensen ◽  
E Dabelsteen
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Acinar Cells ◽  
Salivary Gland Tumors ◽  
Basal Cells ◽  
T Antigens ◽  
Glycosylation Pattern ◽  
Carbohydrate Antigens ◽  
Major Salivary Glands ◽  
Duct Cells

Simple mucin-type carbohydrate antigens Tn, sialosyl-Tn and T are often markers of neoplastic transformation and have very limited expression in normal tissues. We performed an immunohistological study of simple mucin-type carbohydrate antigens, including H and A variants, with well-defined monoclonal antibodies (MAb) on frozen and paraffin-embedded normal salivary gland tissue from 22 parotid, 14 submandibular, six sublingual, and 13 labial glands to elucidate the simple mucin-type glycosylation pattern in relation to cyto- and histodifferentiation. The investigated carbohydrate structures were predominantly observed in the cell cytoplasm, most often in the supranuclear area, suggesting localization to the Golgi region, whereas ductal contents were unstained. Mucous acinar cells expressed Tn, sialosyl-Tn, and H and A antigens, regardless of glandular location. Serous acinar cells, on the other hand, expressed A, H, and inconstantly sialosyl-T, Tn, and sialosyl-Tn antigens in major salivary glands, whereas serous cells of minor (labial) salivary glands expressed H exclusively, Tn and sialosyl-T antigens inconstantly, but never sialosyl-Tn and A antigens. The difference may be related to a more simple cytodifferentiation of serous cells of minor (labial) salivary glands as compared with major salivary glands. Duct cells in major salivary glands expressed A, H, and inconstantly T, sialosyl-T, and Tn antigens, whereas minor (labial) salivary glands ducts exclusively expressed H, T and sialosyl-T antigens, differences that may be related to dissimilarities in the duct system. Myoepithelial cells and basal cells exclusively expressed T and sialosyl-T antigens, which may prove useful in studies of salivary gland tumors, since these cells are known to play a key role in the histological characteristics of some salivary gland tumors. The results indicate a similar glycosylation pattern in the different major salivary glands, whereas minor (labial) salivary gland differ slightly in serous and duct cells. The limited and exclusive intracellular expression of the immature Tn, sialosyl-Tn, and T antigens indicates that these structures may be of value as markers of salivary gland tumors.

scholarly journals Effects of dietary cholesterol in the Mongolian gerbil and the rat: a comparative study

1989 ◽  
Vol 23 (1) ◽  
pp. 30-35 ◽  
Author(s):  
A. M. Temmerman ◽  
R. J. Vonk ◽  
K. Niezen-Koning ◽  
R. Berger ◽  
J. Fernandes
Keyword(s):  
Comparative Study ◽  
Mongolian Gerbil ◽  
Cholesterol Ester ◽  
Dietary Cholesterol ◽  
Hepatic Cholesterol ◽  
Cholesterol Feeding ◽  
Acidic Steroid ◽  
To Come ◽  
Steroid Excretion ◽  
Low Cholesterol

To come to a better understanding of the diet-induced cholesterol-ester storage in the gerbil liver, the reactions of the gerbil to 0·2% of cholesterol in the diet during 4 weeks were compared with those of the rat consuming the same diet. The major reason for the increased hepatic cholesterol-ester storage in the cholesterol-fed gerbil is the low cholesterol turnover in this species. This contrasts with the rat. Although faecal acidic steroid excretion can be slightly increased during cholesterol feeding in the gerbil, this increase is not sufficient to compensate for the quantity of dietary cholesterol when administered at the 0·2% level.

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