scholarly journals Morphological Study of Cell Injury in the Pancreas Induced by Alloxan as Revealed by Labeling of Fragmented DNA (TUNEL Assay).

2000 ◽  
Vol 33 (2) ◽  
pp. 109-113 ◽  
Author(s):  
Masaru Kimura ◽  
Hideto Kawamura ◽  
Takashi Nakano
Keyword(s):  
Morphological Study ◽  
Cell Injury ◽  
Tunel Assay ◽  
Fragmented Dna

scholarly journals TUNEL Assay: A Powerful Tool for Kidney Injury Evaluation

2021 ◽  
Vol 22 (1) ◽  
pp. 412
Author(s):  
Christopher L. Moore ◽  
Alena V. Savenka ◽  
Alexei G. Basnakian
Keyword(s):  
Cell Death ◽  
Dna Fragmentation ◽  
Cell Injury ◽  
Kidney Injury ◽  
Cell Types ◽  
Tunel Assay ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Hypoxic Injury ◽  
Additional Information ◽  
Tissue Compartments

Terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay is a long-established assay used to detect cell death-associated DNA fragmentation (3’-OH DNA termini) by endonucleases. Because these enzymes are particularly active in the kidney, TUNEL is widely used to identify and quantify DNA fragmentation and cell death in cultured kidney cells and animal and human kidneys resulting from toxic or hypoxic injury. The early characterization of TUNEL as an apoptotic assay has led to numerous misinterpretations of the mechanisms of kidney cell injury. Nevertheless, TUNEL is becoming increasingly popular for kidney injury assessment because it can be used universally in cultured and tissue cells and for all mechanisms of cell death. Furthermore, it is sensitive, accurate, quantitative, easily linked to particular cells or tissue compartments, and can be combined with immunohistochemistry to allow reliable identification of cell types or likely mechanisms of cell death. Traditionally, TUNEL analysis has been limited to the presence or absence of a TUNEL signal. However, additional information on the mechanism of cell death can be obtained from the analysis of TUNEL patterns.

Morphological study of fragmented DNA on touched objects

2008 ◽  
Vol 3 (1) ◽  
pp. 32-36 ◽  
Author(s):  
Toshiro Kita ◽  
Hiroki Yamaguchi ◽  
Mitsuru Yokoyama ◽  
Toshiko Tanaka ◽  
Noriyuki Tanaka
Keyword(s):  
Morphological Study ◽  
Fragmented Dna

scholarly journals In situ detection of fragmented dna (tunel assay) fails to discriminate among apoptosis, necrosis, and autolytic cell death: A cautionary note

Hepatology ◽  
1995 ◽  
Vol 21 (5) ◽  
pp. 1465-1468 ◽  
Author(s):  
Bettina Grasl Kraupp ◽  
Branislav Ruttkay-Nedecky ◽  
Helga Koudelka ◽  
Krystyna Bukowska ◽  
Wilfried Bursch ◽  
...  
Keyword(s):  
Cell Death ◽  
Tunel Assay ◽  
Cautionary Note ◽  
Fragmented Dna ◽  
In Situ Detection

A novel method to determine specificity and sensitivity of the TUNEL reaction in the quantitation of apoptosis

2003 ◽  
Vol 284 (5) ◽  
pp. C1309-C1318 ◽  
Author(s):  
K. J. Kelly ◽  
Ruben M. Sandoval ◽  
Kenneth W. Dunn ◽  
Bruce A. Molitoris ◽  
Pierre C. Dagher
Keyword(s):  
Cell Injury ◽  
Tunel Assay ◽  
Live Cells ◽  
Tunel Staining ◽  
Experimental Conditions ◽  
Simple Method ◽  
Specificity And Sensitivity ◽  
Tunel Reaction

Apoptosis is an important mode of cell death under both physiological and pathophysiological conditions. Numerous techniques are available for the study and quantitation of apoptosis in cell culture, but only few are useful when applied to complex tissues. Among these, the terminal transferase-mediated dUTP nick end-labeling (TUNEL) assay remains the most widely used technique. However, its specificity and sensitivity for the detection of apoptosis remain controversial. We developed a technique consisting of staining live cells and tissues with Hoechst 33342 and the vital dye propidium iodide (PI), followed by fixation and the TUNEL reaction. We demonstrate excellent retention of PI in necrotic cells after fixation. We also examined the distribution of TUNEL staining among necrotic and apoptotic cells in various models of cell injury in vitro and in vivo. We show that the sensitivity of the TUNEL varied between 61 and 90% in the models examined. The specificity exceeded 87% in all models but fell to 70% when a predominantly necrotic injury was induced. This novel and simple method will permit the determination of indices of sensitivity and specificity for the TUNEL assay in other tissues and experimental conditions.

Techniques for preparing the Lolium perenne coleorhiza for scanning electron microscope evaluation

1992 ◽  
Vol 50 (1) ◽  
pp. 766-767
Author(s):  
Susan B.G. Debaene ◽  
John S. Gardner ◽  
Phil S. Allen
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Lolium Perenne ◽  
Morphological Study ◽  
Root Hairs ◽  
Inner Pressure ◽  
Water Potentials ◽  
Radicle Emergence ◽  
Standard Fixation ◽  
Scanning Electron

The coleorhiza is a nonvascular sheath that encloses the embryonic radicle in Poaceae, and is generally the first tissue to emerge during germination. Delicate hairlike extensions develop from some coleorhiza cells prior to radicle emergence. Similar to root hairs, coleorhiza hairs are extremely sensitive to desiccation and are damaged by exposure to negative water potentials. The coleorhiza of Lolium perenne is somewhat spherical when first visible, after which a knob forms at a right angle to the caryopsis due to inner pressure from the elongating radicle. This knob increases in length until the radicle finally punctures the coleorhiza. Standard fixation procedures cause severe desiccation of coleorhiza cells and hairs, making morphological study of the coleorhiza difficult. This study was conducted to determine a more successful process for coleorhiza preservation.

Intracellular EDEMA does not adversely affect the ability to cryopreserve intact hearts

1993 ◽  
Vol 51 ◽  
pp. 278-279
Author(s):  
CL Hastings ◽  
RD Carlton ◽  
FG Lightfoot ◽  
AF Tryka
Keyword(s):  
Cell Injury ◽  
Median Sternotomy ◽  
Left Ventricular ◽  
Ventricular Free Wall ◽  
Hypoxic Cell ◽  
Free Wall ◽  
Sprague Dawley ◽  
Left Ventricular Free Wall ◽  
Sprague Dawley Rats

The earliest ultrastructural manifestation of hypoxic cell injury is the presence of intracellular edema. Does this intracellular edema affect the ability to cryopreserve intact myocardium? To answer this guestion, a model for anoxia induced intracellular edema (IE) was designed based on clinical intraoperative myocardial preservation protocol. The aortas of 250 gm male Sprague-Dawley rats were cannulated and a retrograde flush of Plegisol at 8°C was infused over 90 sec. The hearts were excised and placed in a 28°C bath of Lactated Ringers for 1 h. The left ventricular free wall was then sliced and the myocardium was slam frozen. Control rats (C) were anesthetized, the hearts approached by median sternotomy, and the left ventricular free wall frozen in situ immediately after slicing. The slam frozen samples were obtained utilizing the DDK PS1000, which was precooled to -185°C in liguid nitrogen. The tissue was in contact with the metal mirror for a dwell time of 20 sec, and stored in liguid nitrogen until freeze dry processing (Lightfoot, 1990).

Sign in / Sign up

Export Citation Format

Share Document