scholarly journals Changes in the Cyclin D1 Gene Copy Number in Melanoma Cell Lines Detected by Double-Target Fluorescence In Situ Hybridization.

1999 ◽  
Vol 32 (5) ◽  
pp. 431-436 ◽  
Author(s):  
Mayumi Matsuta ◽  
Morimasa Matsuta ◽  
Toshihide Akasaka ◽  
Teruo Kagabu
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Cyclin D1 ◽  
Cell Lines ◽  
Melanoma Cell ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Melanoma Cell Lines

scholarly journals TERT and AURKA Gene Copy Number Gains Enhance the Detection of Acral Lentiginous Melanomas by Fluorescence in Situ Hybridization

2014 ◽  
Vol 16 (2) ◽  
pp. 198-206 ◽  
Author(s):  
Alba Diaz ◽  
Joan Anton Puig-Butillé ◽  
Alexandra Valera ◽  
Concha Muñoz ◽  
Dolors Costa ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy

scholarly journals Anatomic site-specific patterns of gene copy number gains in skin, mucosal, and uveal melanomas detected by fluorescence in situ hybridization

2006 ◽  
Vol 449 (3) ◽  
pp. 328-333 ◽  
Author(s):  
Katharina Glatz-Krieger ◽  
Mona Pache ◽  
Coya Tapia ◽  
Alain Fuchs ◽  
Spasenija Savic ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Anatomic Site ◽  
Site Specific ◽  
Uveal Melanomas

Copy Number Analysis of c-erb-B2 (HER-2/neu) and Topoisomerase IIα Genes in Breast Carcinoma by Quantitative Real-Time Polymerase Chain Reaction Using Hybridization Probes and Fluorescence In Situ Hybridization

2005 ◽  
Vol 129 (1) ◽  
pp. 39-46
Author(s):  
Sabita K. Murthy ◽  
Anthony M. Magliocco ◽  
Douglas J. Demetrick
Keyword(s):  
Breast Cancer ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Real Time ◽  
Copy Number ◽  
Gene Copy Number ◽  
Gene Copy ◽  
Hybridization Probes ◽  
Fresh Frozen

Abstract Context.—The Topoisomerase IIα (TOP2A) protein is the target of the anthracycline class of chemotherapeutic agents. TOP2A is frequently coamplified with c-erb-B2 and consequently might be a prognostic and/or predictive factor for breast cancer patients when anthracycline-based chemotherapy is a consideration. A total of 20% to 35% of breast carcinomas show amplification of the erb-B2 gene, some of which also have coamplification of the TOP2A gene. Investigation of the prognostic or predictive significance of these gene amplifications requires a reliable and sensitive method for the measurement of gene copy number in clinical tumor samples. Objective.—To assess 2 different assay methods that might allow accurate, reproducible, quantitative, and high-throughput estimation of gene copy number in fresh, frozen, or paraffin-embedded breast cancer specimens. Design.—We developed an assay and analyzed the gene copy numbers of the erb-B2 and TOP2A genes in 8 breast cancer cell lines, 6 fresh frozen samples, and 38 paraffin-embedded breast tumor specimens by a novel real-time polymerase chain reaction (PCR) assay using hybridization probes. The results were compared with standard fluorescence in situ hybridization. Results.—We discovered a 100% concordance between assessment of gene copy number of erb-B2 and TOP2A between quantitative PCR and fluorescence in situ hybridization (FISH). Quantitative PCR also had the additional feature of uncovering an erb-B2 gene polymorphism. Finally, we observed that TOP2A amplification only occurred in conjunction with erb-B2 amplification in our paraffin-embedded cases of invasive breast carcinoma and that this event was present in 5 (42%) of 12 erb-B2 amplified cases. Conclusions.—We conclude that the potentially automatic, real-time PCR analysis using hybridization probes is an efficient method to perform copy number analysis, with results that appear identical to the FISH technique and with the benefit of identifying HER-2 polymorphisms.

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