scholarly journals Microwave Fixation and Localization of Calcium in Synaptic Terminals and Muscular Cells by Electron Probe X-Ray Microanalysis and Electron Energy-Loss Spectroscopy Imaging.

1997 ◽  
Vol 30 (3) ◽  
pp. 277-301 ◽  
Author(s):  
Vinci Mizuhira ◽  
Hiroshi Hasegawa ◽  
Mitsuru Notoya
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Electron Energy Loss Spectroscopy ◽  
Electron Probe ◽  
X Ray ◽  
Synaptic Terminals ◽  
Microwave Fixation ◽  
Electron Energy Loss

Electron probe analysis, x-ray mapping and electron energy loss spectroscopy of elemental distribution in Escherichia coli B

1984 ◽  
Vol 42 ◽  
pp. 570-571
Author(s):  
Chung-Fu Chang ◽  
Henry Shuman ◽  
Andrew P. Somlyo
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Electron Energy Loss Spectroscopy ◽  
Electron Probe ◽  
Bright Field Image ◽  
X Ray ◽  
E Coli ◽  
Specimen Holder ◽  
Freeze Dried ◽  
Electron Energy Loss

The ability of bacteria to concentrate minerals is well known, but little is known about the subcellular distribution of ions. Electron probe microanalysis, X-ray mapping and electron energy loss spectroscopy of ultrathin cryosections are particularly suitable for determining composition at the ultrastructural level in cells, including bacterial spores. In the present study, we report preliminary experiments with these methods on elemental concentrations and distribution in E. coli B, including differences in the calcium content between dividing and non-dividing cells.E. coli B were grown in the presence of 1% tryptone (DIFCO), 0.2% glucose and 0.1M NaCl. The cells were harvested at a concentration of ∽3x108 cell/ml (in the late log phase). Cells were washed in nominallv ion-free solutions and then frozen, in the presence of 10% PVP, in supercooled Freon 22 at -164°C. The specimens were freeze-dried and cryosectloned as described previously. Analyses were performed on a Gatan LN2 cold specimen holder, at a temperature of -101°C. A bright-field image of the cryosectioned E. coli is shown in Fig. 1.

Signal/Noise Ratio and Elemental Detectivity by Eels

1982 ◽  
Vol 40 ◽  
pp. 488-489
Author(s):  
R. F. Egerton
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Elemental Analysis ◽  
Electron Energy Loss Spectroscopy ◽  
Emission Spectroscopy ◽  
X Ray ◽  
Signal Noise ◽  
Characteristic Signal ◽  
Noise Ratio ◽  
Electron Energy Loss

An important parameter governing the sensitivity and accuracy of elemental analysis by electron energy-loss spectroscopy (EELS) or by X-ray emission spectroscopy is the signal/noise ratio of the characteristic signal.

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