scholarly journals Localization with a Digoxigenin-Labeled cRNA Probe of Basic Fibroblast Growth Factor mRNA in Dystrophic (mdx) Mouse Masseter Muscle and Rat Hair Follicles.

1993 ◽  
Vol 26 (5) ◽  
pp. 381-389
Author(s):  
KUNIHIRO URYU ◽  
HIROSHI IWATA ◽  
SHIGETAKA YOSHIDA ◽  
SADAO SHIOSAKA ◽  
SEIJI MATSUDA ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Masseter Muscle ◽  
Hair Follicles ◽  
Mdx Mouse ◽  
Fibroblast Growth ◽  
Crna Probe

Loss of cytoplasmic basic fibroblast growth factor from physiologically wounded myofibers of normal and dystrophic muscle

1993 ◽  
Vol 106 (1) ◽  
pp. 121-133 ◽  
Author(s):  
M.S. Clarke ◽  
R. Khakee ◽  
P.L. McNeil
Keyword(s):  
Plasma Membrane ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Plasma Membranes ◽  
Mdx Mouse ◽  
Storage Site ◽  
Fibroblast Growth ◽  
Mouse Muscle ◽  
Cellular Environment

Using muscle as an in vivo model system, we have tested the hypothesis that basic fibroblast growth factor is released from a cytoplasmic storage site into the extra-cellular environment via diffusion through survivable, mechanically-induced plasma membrane disruptions. Normal and dystrophic (mdx) mouse muscle were studied. Strong immunostaining for bFGF was detected in the cytoplasm of myofibers of uninjured muscle fixed in situ by perfusion. By contrast, myofibers did not stain cytoplasmically for bFGF after suffering lethal disruptions of their plasma membranes caused by freezing and thawing followed by sectioning. Sub-lethal, transient disruptions of myofiber plasma membranes--termed plasma membrane ‘wounds’--were shown to be induced by needle puncture or exercise of muscle. Quantitative image analysis revealed that these wounded fibers contained significantly reduced levels of bFGF. Dystrophic exercised and unexercised muscle was found to possess an approximately 6-fold higher proportion of wounded myofibers than does normal muscle under equivalent conditions. Release of bFGF at a rate that is a direct function of the frequency of myofiber wounding may explain in part how a muscle adjusts its growth to meet changing mechanical demand as well as the pathological hypertrophy characteristic of certain stages of muscular dystrophy.

A Case of Contact Dermatitis due to Fiblast Spray, a Product of Basic Fibroblast Growth Factor

2006 ◽  
Vol 68 (3) ◽  
pp. 248-250 ◽  
Author(s):  
Shuko OKADA ◽  
Takashi MASU ◽  
Takahiko TSUNODA ◽  
Ryuhei OKUYAMA ◽  
Setsuya AIBA
Keyword(s):  
Contact Dermatitis ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Basic Fibroblast Growth Factor ◽  
Fibroblast Growth
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