scholarly journals Double staining procedure for histochemical localization of alcohol and aldehyde dehydrogenase activities in the mouse liver.

1989 ◽  
Vol 22 (3) ◽  
pp. 401-406 ◽  
Author(s):  
TOSHIMITSU WATABIKI ◽  
TAKUMA TOKIYASU ◽  
NORIAKI ISHIDA ◽  
KAZUO OGAWA
Keyword(s):  
Aldehyde Dehydrogenase ◽  
Mouse Liver ◽  
Histochemical Localization ◽  
Staining Procedure ◽  
Double Staining

A pre-embedding double staining procedure used to observe the effect of fixation on the activity of intercellular adhesion molecule on T and B cells

1990 ◽  
Vol 48 (3) ◽  
pp. 378-379
Author(s):  
Jane E. Ramberg ◽  
Shigeto Tohma ◽  
Peter E. Lipsky
Keyword(s):  
B Cells ◽  
Adhesion Molecule ◽  
Colloidal Gold ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Staining Procedure ◽  
Double Staining ◽  
T And B Cells ◽  
Microtiter Plates ◽  
Streptavidin Peroxidase

Intercellular adhesion molecule (ICAM-1) appears to be a ligand for LFA-1 dependent adhesion in T cell mediated cytotoxcity. It is found on cells of both hematopoietic and non-hematopoietic origin. While observing the activity of ICAM-1 on the surfaces of interacting T and B cells, we found that we could successfully carry out a pre-embedding double staining procedure utilizing both colloidal gold and peroxidase conjugated reagents.On 24-well microtiter plates, mitomycin-treated T4 cells were stimulated with 64.1 (anti-CD3) for one hour before the addition, in some instances, of B cells. Following a 12-48 hour incubation at 38°C, the cells were washed and then immunostained with a colloidal gold conjugated RFB-4 (anti-CD22); biotinylated R6.5 (anti-ICAM-1); followed by streptavidin/peroxidase. This method allowed us to observe two different antigens without concern about possible cross-reaction of reagents. Because we suspected ICAM-1 and R6.5 were sensitive to fixation, we tried varying concentrations of fresh paraformaldehyde before R6.5, after R6.5 and after streptavidin/peroxidase. All immunostaining and washing was done on ice with ice cold reagents.

scholarly journals Double-staining procedure for the fluorescent treponemal antibody absorption (FTA-ABS) test.

1979 ◽  
Vol 55 (2) ◽  
pp. 105-108
Author(s):  
E F Hunter ◽  
R M McKinney ◽  
S E Maddison ◽  
D D Cruce
Keyword(s):  
Staining Procedure ◽  
Double Staining ◽  
Antibody Absorption

scholarly journals A simple method for immunofluorescent double staining with primary antisera from the same species.

1993 ◽  
Vol 41 (4) ◽  
pp. 627-630 ◽  
Author(s):  
S Würden ◽  
U Homberg
Keyword(s):  
Schistocerca Gregaria ◽  
Staining Procedure ◽  
Double Staining ◽  
Double Immunofluorescence ◽  
Double Labeling ◽  
Simple Method ◽  
Fluorescent Markers ◽  
Secondary Antiserum ◽  
Secondary Antibodies ◽  
Texas Red

We have developed a new double immunofluorescence technique by which two neuroactive substances in the same tissue section can be labeled with primary antisera raised in the same species. The optic lobes of the locust Schistocerca gregaria were used as a model system to develop the staining procedure. FMRFamide-immunoreactive neurons were detected by rabbit antisera against FMRFamide and FITC-conjugated secondary antibodies. Antibodies against the second peptide, pigment-dispersing hormone (PDH), also raised in rabbit, were biotinylated and detected via streptavidin-Texas Red. Crossreactivity of the PDH immunoglobulins with the FITC-conjugated secondary antiserum was prevented by pre-incubation with rabbit gamma globulins. The two peptide immunoreactivities could be conveniently observed on the same section with the different fluorescent markers. This double labeling technique with modified antibodies is easily performed and highly useful for co-localization studies with antisera raised in the same species.

scholarly journals Alcohol preference and aldehyde dehydrogenase isozymes in mouse liver

1981 ◽  
Vol 31 ◽  
pp. 258
Author(s):  
Hiromi Yamazaki ◽  
Keiko Nishiguchi ◽  
Suehiro Nakanishi
Keyword(s):  
Aldehyde Dehydrogenase ◽  
Mouse Liver ◽  
Alcohol Preference

Aldehyde dehydrogenase as a marker for chemically initiated mouse liver tumors

1986 ◽  
Vol 31 (2) ◽  
pp. 205-211 ◽  
Author(s):  
Raymond E. Richmond ◽  
Michael A. Pereira
Keyword(s):  
Aldehyde Dehydrogenase ◽  
Mouse Liver ◽  
Liver Tumors

scholarly journals An indirect immunofluorescence double staining procedure for the simultaneous flow cytometric measurement of iodo- and chlorodeoxyuridine incorporated into DNA

Cytometry ◽  
1991 ◽  
Vol 12 (4) ◽  
pp. 366-372 ◽  
Author(s):  
P. J. M. Bakker ◽  
J. Stap ◽  
C. J. Tukker ◽  
C. H. van Oven ◽  
C. H. N. Veenhof ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Staining Procedure ◽  
Double Staining ◽  
Flow Cytometric ◽  
Flow Cytometric Measurement
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