scholarly journals Use of AMP(2-amino-2-methyl-1-propanol) buffer for histochemical demonstration of alkaline phosphatase activity.

1988 ◽  
Vol 21 (4) ◽  
pp. 321-326 ◽  
Author(s):  
YU XIU SHI ◽  
NOBUKAZU ARAKI ◽  
TOYOSHI FUJIMOTO ◽  
KAZUO OGAWA
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Histochemical Demonstration

scholarly journals Simultaneous autoradiographic and histochemical analysis of bone formed in vitro.

1986 ◽  
Vol 34 (6) ◽  
pp. 769-773 ◽  
Author(s):  
H C Tenenbaum ◽  
C A McCulloch ◽  
K Palangio
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Time Course ◽  
Bone Cell ◽  
Histochemical Demonstration ◽  
Thymidine Uptake ◽  
Kinetics In Vivo

The simultaneous histochemical demonstration of alkaline phosphatase activity and autoradiographic demonstration of [3H]-thymidine uptake is valuable for study of bone cell kinetics in vivo or in vitro. By use of this technique, it has been possible to detect changes induced by a single dose of dexamethasone (10(-7) M) in the time course of alkaline phosphatase activity, the number of alkaline phosphatase-positive cells, and [3H]-thymidine labeling in bone formed in vitro.

scholarly journals LOCALIZED AREAS OF HIGH ALKALINE PHOSPHATASE ACTIVITY IN THE TERMINAL ARTERIAL TREE

1962 ◽  
Vol 15 (1) ◽  
pp. 73-84 ◽  
Author(s):  
Flaviu C. A. Romanul ◽  
Roger G. Bannister
Keyword(s):  
Alkaline Phosphatase ◽  
Enzymatic Activity ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Histochemical Demonstration ◽  
Capillary Endothelium ◽  
Parent Vessel ◽  
Arterial Tree ◽  
Fresh Frozen ◽  
Cryostat Sections

Fresh frozen skeletal muscles of rats, rabbits, and humans were sectioned in a cryostat. Sections 12 to 32 micra thick were incubated in a substrate solution for the histochemical demonstration of non-specific alkaline phosphatase activity. A modified azo dye coupling technique was used at pH 9.5. Localized areas of high enzymatic activity were found in specific and well defined areas along the terminal arterial tree, in addition to the activity which has been previously described in capillary endothelium. Arterial branches with luminal diameters of 25 micra or less showed staining of their endothelium starting abruptly at their origin from the parent vessel and fading distally. Smaller arterial branches showed the same localization of enzymatic activity and stained more intensely. Other organs of rats surveyed showed arterial branches with the same pattern of staining. Identical results were obtained using the Gomori technique for alkaline phosphatase. Extensive saline perfusion of the vascular tree did not affect the observed localization of enzymatic activity. The enzymatic activity described may be part of the mechanism regulating the blood flow.

LEUCOCYTE METABOLISM IN PREGNANCY

1960 ◽  
Vol XXXV (IV) ◽  
pp. 575-584 ◽  
Author(s):  
C. Borel ◽  
J. Frei ◽  
A. Vannotti
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Consumption ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lactate Production ◽  
Glucose Consumption ◽  
In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

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