scholarly journals Preparation of monoclonal antibody to rabbit kidney gamma-glutamyl transpeptidase and its cross-reactivity analysis in immunohistochemistry.

1987 ◽  
Vol 20 (2) ◽  
pp. 163-176 ◽  
Author(s):  
TOSHIKO KOMATSU ◽  
SHUJI YAMASHITA ◽  
KENJIRO YASUDA
Keyword(s):  
Monoclonal Antibody ◽  
Cross Reactivity ◽  
Rabbit Kidney ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Glutamyl Transpeptidase

scholarly journals A monoclonal antibody against human kidney gamma-glutamyl transpeptidase: preparation, immunochemical, and immunohistochemical characterization.

1989 ◽  
Vol 37 (7) ◽  
pp. 1053-1061 ◽  
Author(s):  
M Shiozawa ◽  
S Yamashita ◽  
S Aiso ◽  
K Yasuda
Keyword(s):  
Monoclonal Antibody ◽  
Immunohistochemical Study ◽  
Basolateral Membrane ◽  
Human Kidney ◽  
Normal Liver ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Human Organs ◽  
Human Enzyme ◽  
Glutamyl Transpeptidase

To perform immunohistochemical study of the distribution of gamma-glutamyl transpeptidase in human organs, a highly specific antibody against the human enzyme is required. We prepared monoclonal antibody against gamma-glutamyl transpeptidase from human kidney, using the hybridoma technique. The antibody was of the IgG1 type and the light chain belonged to the kappa class. The antibody reacted specifically with the 63 KD heavy subunit of the enzyme. Examination of the specificity of the antibody performed by immunohistochemical staining of human kidney sections revealed that the antigen was localized on the brush border and along the basolateral membrane of the epithelial cells of both the convoluted and the straight portions of the proximal tubule. This antibody was also reactive in several human organs other than kidney, including epididymis, prostate, seminal vesicle, pancreas, and normal liver, and in human hepatoma. These findings indicate the existence of an antigenic determinant common to human kidney and other organs. The monoclonal antibody did not crossreact with mouse, rat, guinea pig, rabbit, or pig kidney.

scholarly journals Distribution of gamma-glutamyl transpeptidase in human pancreas: immunohistochemical study with a monoclonal antibody.

1990 ◽  
Vol 38 (3) ◽  
pp. 339-350 ◽  
Author(s):  
K Yasuda ◽  
M Shiozawa ◽  
S Aiso ◽  
S Taniguchi ◽  
S Yamashita
Keyword(s):  
Monoclonal Antibody ◽  
Immunohistochemical Study ◽  
Islet Cells ◽  
Human Kidney ◽  
Immunoperoxidase Technique ◽  
Human Pancreas ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Glutamyl Transpeptidase ◽  
Centroacinar Cells

We studied in detail the distribution pattern of gamma-glutamyl transpeptidase (gamma-GT) in human pancreas, using the immunoperoxidase technique and a monoclonal antibody to human kidney gamma-GT. Positive reaction was confined exclusively to the luminal surface of the centroacinar cells and the epithelia of the intercalated, intralobular, and interlobular ducts. The immunoreaction was stronger in the intercalated and intralobular ducts than in the interlobular ducts. The acinar cells did not show any reaction. The islets of Langerhans were heavily surrounded by the ducts, and normal islet cells showed no reaction. The course of the ducts, from the acinar lumina to the interlobular ducts, was delineated by using reaction sites positive for gamma-GT as markers. The courses of the ducts, which comprise the pathway for pancreatic juice, were found to vary widely in their connections with each other, especially between the intralobular and interlobular ducts. At least three separate routes could be identified.

scholarly journals Distribution of gamma-glutamyl transpeptidase in human salivary glands: immunohistochemical study using a monoclonal antibody.

1993 ◽  
Vol 41 (2) ◽  
pp. 205-213 ◽  
Author(s):  
M Shiozawa ◽  
K Yasuda ◽  
S Yamashita ◽  
S Aiso
Keyword(s):  
Monoclonal Antibody ◽  
Parotid Gland ◽  
Salivary Glands ◽  
Sublingual Gland ◽  
Parotid Glands ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Luminal Membrane ◽  
Duct Cells ◽  
Glutamyl Transpeptidase

We studied in detail the distribution pattern of gamma-glutamyl transpeptidase (gamma-GT) in human salivary glands using a monoclonal antibody (MAb) to human kidney gamma-GT. In the sublingual gland, a strong reactivity of the enzyme was recognized along the luminal and lateral membranes of serous cells. Weak but positive reactivity was noted on the luminal membrane of mucous cells. The intercellular canaliculi in the demilune and luminal surfaces of excretory duct cells were also immunoreactive. In the submandibular gland, a weak reaction was observed on the luminal membrane of intercalated duct cells and striated duct cells. Faint reactivity was seen on the luminal membrane of striated duct cells of the parotid gland. No reaction was observed in serous cells of the parotid gland. The immunoreaction in the sublingual gland was stronger than that in submandibular or parotid glands.

scholarly journals Localization of gamma-glutamyl transpeptidase in human sweat glands: an immunohistochemical study using a monoclonal antibody.

1990 ◽  
Vol 38 (10) ◽  
pp. 1433-1444 ◽  
Author(s):  
K Yasuda ◽  
M Shiozawa ◽  
S Yamashita ◽  
S Aiso
Keyword(s):  
Monoclonal Antibody ◽  
Eccrine Sweat Gland ◽  
Sweat Glands ◽  
Secretory Cells ◽  
Basal Cells ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Human Sweat ◽  
Luminal Membrane ◽  
Glutamyl Transpeptidase

We studied the distribution of gamma-glutamyl transpeptidase (gamma-GT) by use of a monoclonal antibody (MAb) against human kidney gamma-GT in human sweat glands. In the eccrine sweat gland, the enzyme was localized along the luminal membrane and small apocrine extrusions of the superficial cells of the secretory portion. The intercellular canaliculi between basal cells were occasionally immunoreactive. In the secretory portion of the apocrine gland, luminal membrane and apocrine extrusions of various sizes and stages at the apices of the secretory cells exhibited positive reactions. Immunoreaction was also seen in the Golgi area of the cuboidal secretory cells. No positive reaction was observed in the myoepithelial cells of either gland or in the excretory duct cells.

Relationship Between Gamma-glutamyl Transpeptidase Activity and Inflammatory Response in Lichen Planus

2018 ◽  
Vol 69 (3) ◽  
pp. 739-743 ◽  
Author(s):  
Madalina Irina Mitran ◽  
Ilinca Nicolae ◽  
Corina Daniela Ene ◽  
Cristina Iulia Mitran ◽  
Clara Matei ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Lichen Planus ◽  
Inflammatory Process ◽  
Molecular Mechanisms ◽  
Functional Adaptation ◽  
Physical Factors ◽  
Inflammatory Disorder ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Glutamyl Transpeptidase

Chemicals used in the manufacture of synthetic fibers have been associated with undesirable side effects such as itching or skin lesions and it seems that they are involved in the induction of pathological processes such as oxidative stress and inflammation. Lichen planus (LP) can be regarded as an inflammatory disorder, chemical and physical factors playing an important role in the perpetuation of the inflammatory process. Gamma-glutamyl transpeptidase (GGT) plays an important role in the preservation of skin architecture and modulation of skin inflammation. In this study, we found that GGT activity is increased in LP patients with mild inflammation, whilst GGT is inactivated under conditions of severe inflammation. Therefore, GGT is involved in the inflammatory process, but there is no a positive correlation between its activity and the intensity of the inflammatory response. This functional adaptation of the enzyme may be due to down-regulation of its synthesis under free radical overload conditions. Understanding the molecular mechanisms involved in the modulation of intracellular redox homeostasis is an important step in the pharmacological management of patients with LP.

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