scholarly journals Staining of serous acinar cells of human parotid gland with wheat germ agglutinin-gold complex.

1985 ◽  
Vol 18 (4) ◽  
pp. 391-394 ◽  
Author(s):  
MITSUO MACHINO ◽  
HIROYUKI MORIOKA ◽  
MASAYOSHI TACHIBANA
Keyword(s):  
Parotid Gland ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Acinar Cells ◽  
Gold Complex ◽  
Human Parotid Gland

scholarly journals Formation of post-confluence structure in human parotid gland acinar cells on PLGA through regulation of E-cadherin

Biomaterials ◽  
2012 ◽  
Vol 33 (2) ◽  
pp. 464-472 ◽  
Author(s):  
Yen-Hui Chan ◽  
Tsung-Wei Huang ◽  
Ya-Shuan Chou ◽  
Sheng-Hao Hsu ◽  
Wei-Fang Su ◽  
...  
Keyword(s):  
Parotid Gland ◽  
Acinar Cells ◽  
Human Parotid Gland ◽  
E Cadherin

Fine structure of acinar cells of human parotid gland

1973 ◽  
Vol 176 (2) ◽  
pp. 149-165 ◽  
Author(s):  
Alessandro Riva ◽  
Francesca Riva -Testa
Keyword(s):  
Fine Structure ◽  
Parotid Gland ◽  
Acinar Cells ◽  
Human Parotid Gland

Effects of fibroblasts on the function of acinar cells from the same human parotid gland

Head & Neck ◽  
2015 ◽  
Vol 38 (S1) ◽  
pp. E279-E286 ◽  
Author(s):  
Ya-Shuan Chou ◽  
Yong-Chong Lin ◽  
Tai-Horng Young ◽  
Pei-Jen Lou
Keyword(s):  
Parotid Gland ◽  
Acinar Cells ◽  
Human Parotid Gland

Altered Serous Granules in Acinar Cells of a Human Parotid Gland

1990 ◽  
Vol 14 (1) ◽  
pp. 11-19 ◽  
Author(s):  
Bernard Tandler ◽  
Alessandro Riva
Keyword(s):  
Parotid Gland ◽  
Acinar Cells ◽  
Human Parotid Gland

Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

1977 ◽  
Vol 35 ◽  
pp. 640-641
Author(s):  
J. R. Ruby
Keyword(s):  
Endoplasmic Reticulum ◽  
Parotid Gland ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Duct System ◽  
Parotid Glands ◽  
Dasypus Novemcinctus ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

Failure of 6D1 or Exposure of Platelets to ADP to Affect Agglutination Induced by Wheat Germ Agglutinin

1989 ◽  
Vol 62 (02) ◽  
pp. 815 ◽  
Author(s):  
Marjorie B Zucker ◽  
Robert A Grant ◽  
Evelyn A Mauss
Keyword(s):  
Wheat Germ Agglutinin ◽  
Wheat Germ

Transport Characteristics of Wheat Germ Agglutinin-Modified Insulin-Liposomes and Solid Lipid Nanoparticles in a Perfused Rat Intestinal Model

2006 ◽  
Vol 6 (9) ◽  
pp. 2959-2966 ◽  
Author(s):  
Na Zhang ◽  
Qineng Ping ◽  
Guihua Huang ◽  
Xiuzhen Han ◽  
Yanna Cheng ◽  
...  
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Serum Insulin ◽  
Lipid Nanoparticles ◽  
Solid Lipid ◽  
Mucosal Absorption ◽  
Perfusion Experiment ◽  
Absorption Sites

Wheat germ agglutinin (WGA) modified liposomes and solid lipid nanoparticles (SLNs) were evaluated for improving intestinal absorption of insulin. In an in situ local intestinal perfusion experiment, formulations containing 100 IU/kg insulin were administered to the duodenum, jejunum, and ileum of fasted rats. As hypothesized, ileum was the best intestinal location for the absorption of insulin-containing liposomes. Serum insulin concentrations decreased for the various formulations in different absorption sites according to the following trends: Duodenum > ileum > jejunum for WGA-modified insulin-containing liposomes; duodenum > jejunum > ileum for WGA-modified insulin-containing SLNs; ileum > jejunum > duodenum for insulin-containing liposomes; ileum > duodenum > jejunum for insulin-containing SLNs; and duodenum ≥ ileum > jejunum for aqueous solution of insulin. These results imply that the nanoparticle type and delivery site were important factors with respect to increasing the bioavailability of insulin following oral administration. The proteolytic degradation as well as the epithelial permeability were primary determinants influcing insulin mucosal absorption.

Identification of distinct haemocyte populations from the freshwater bivalves swan mussel (Anodonta cygnea) and duck mussel (Anodonta anatina) using wheat-germ agglutinin

2017 ◽  
Vol 95 (12) ◽  
pp. 937-947 ◽  
Author(s):  
M. Hinzmann ◽  
M. Lopes-Lima ◽  
F. Cerca ◽  
A. Correia ◽  
J. Machado ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Transmission Microscopy ◽  
Anodonta Cygnea ◽  
Functional Studies ◽  
Lectin Staining ◽  
Surface Staining ◽  
Cytological Features ◽  
Freshwater Bivalves

Haemocytes play a major role in molluscs immunity. Functional studies are, however, impaired by limited available experimental tools to identify and sort distinct haemocyte populations. Therefore, using nonlethal methods, we aimed at evaluating whether lectin staining combined with flow cytometry could be used to distinguish circulating haemocyte populations from two freshwater bivalves of the family Unionidae, the duck mussel (Anodonta anatina (L., 1758)) and the swan mussel (Anodonta cygnea (L., 1758)). Based on classical classification, haemocytes were distinguished as granulocytes and hyalinocytes and cytological features were visualized using transmission microscopy and staining techniques. Size, granularity, viability, and surface staining using lectins as specific probes were analysed by flow cytometry and fluorescence microscopy. The microscopic proportions of granulocytes and hyalinocytes significantly differed, being of 70% and 30% for A. cygnea and of 85% and 15% for A. anatina, respectively. Two haemocyte populations were sorted by flow cytometry based on size and granularity and confirmed as granulocytes and hyalinocytes. Interestingly, two different granulocyte populations could be further discriminated in A. cygnea according to their binding affinity to wheat-germ agglutinin (WGA), whereas granulocytes of A. anatina all stained similarly. Our results show that WGA labelling combined with flow cytometry can be used to better discriminate Anodonta haemocyte populations and obtain purified populations for functional studies.

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