scholarly journals Histologic staining methods for hepatitis B surface antigen (HBS AG) and interpretation of dye reactions in vitro.

1984 ◽  
Vol 17 (3) ◽  
pp. 241-249
Author(s):  
MASACHIKA SENBA
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Staining Methods ◽  
Hbs Ag

Stability and Activity of Intravenous Immunoglobulin with Neonatal Dextrose and Total Parenteral Nutrient Solutions

1994 ◽  
Vol 28 (9) ◽  
pp. 1014-1017
Author(s):  
Christine A. Lindsay ◽  
Ken Dang ◽  
James M. Adams ◽  
Ching Nan Ou ◽  
Carol J. Baker
Keyword(s):  
Hepatitis B ◽  
Intravenous Immunoglobulin ◽  
Surface Antigen ◽  
Immunoglobulin G ◽  
Functional Activity ◽  
Hepatitis B Surface Antigen ◽  
Type Iii ◽  
Apparent Decrease ◽  
Total Immunoglobulin

OBJECTIVE: To determine in vitro the compatibility of reconstituted intravenous immunoglobulin (IVIG) (Gammagard, Baxter-Hyland) with five different neonatal and pediatric intravenous solutions in Viaflex polyvinyl chloride bags. DESIGN: In vitro compatibility study. INTERVENTIONS: Samples were taken at time=0, 10, 30, 60, 90, and 120 minutes and at 4, 8, 12, and 24 hours and assayed for total immunoglobulin G content and antibodies to hepatitis B surface antigen. Type III group B Streptococcus (GBS) and opsonic activity for type III GBS were analyzed at time=0, 60, and 120 minutes and 12 and 24 hours. All results were compared with those from pure IVIG. RESULTS: Our results demonstrate that mixing IVIG with intravenous solutions commonly used in the care of premature infants (dextrose 5% in water [D5W], D15W, D5W/NaCl 0.225%, and total parenteral nutrition [TPN]) does not significantly alter total immunoglobulin G concentrations or concentration of antibodies to hepatitis B surface antigen or type III GBS. As well, the in vitro functional activity for type III GBS of the IVIG, when mixed with these solutions for up to 24 hours, remained intact. An apparent decrease in bactericidal killing was seen with the IVIG/central TPN mixture. This apparent decrease was found to be an artifact of the high concentration of glucose (20 percent) in the solution. CONCLUSIONS: We propose that Gammagard may be mixed with these solutions through Y-site connections without loss of antibody content or functional activity of the IVIG.

478 KINETICS OF SERUM HEPATITIS B SURFACE ANTIGEN LEVEL IN GENOTYPE C HBS AG SEROCLEARED CARRIERS

2012 ◽  
Vol 56 ◽  
pp. S188
Author(s):  
S. Nagaoka ◽  
H. Yatsuhashi ◽  
Y. Kugiytama ◽  
K. Ogihara ◽  
S. Bekki ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Antigen Level ◽  
Serum Hepatitis ◽  
Genotype C ◽  
Hbs Ag ◽  
Kinetics Of

Conjugates of Hepatitis B Surface Antigen (14-32)Pre-S2 Region with N-Acetylmuramic Acid, N-Acetylnormuramic Acid, and Their L-Alanyl-D-Isoglutamine Derivatives: Synthesis and Studies on Immunogenicity

1992 ◽  
Vol 57 (1) ◽  
pp. 204-211
Author(s):  
Zbigniev Maćkiewicz ◽  
Hanna Świderska ◽  
Maria Kalmanowa ◽  
Zygfryd Smiatacz ◽  
Adam Nowosławski ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
Phase Method ◽  
Virus Surface ◽  
Solid Phase Method ◽  
Cellular And Humoral Immunity ◽  
Cellular Immune

Four novel analogs of hepatitis B virus surface antigen (14-32)Pre-S2 region fragment attached covalently to N-acetylmuramic acid, N-acetylmuramyl-L-alanyl-D-isoglutamine, N-acetylnormuramic acid, and N-acetylnormuramyl-L-alanyl-D-isoglutamine were synthesized by the solid phase method. The ability of analogs to induce cellular and humoral immunity to native HBsAg was tested on rabbits. Cellular immune response occurred in vitro, and HBs antibodies were detected in all immunized animals. No additional adjuvants were used in the tests.

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