Separation and Characterization of a Monoclonal IgG2 Antibody by Cation Exchange Chromatography

2003 ◽  
Vol 2 (6) ◽  
pp. 37-43 ◽  
Author(s):  
Yuling Zhang ◽  
Andrew Goetze ◽  
Julia Boyce ◽  
Mary Gerhart ◽  
Shawn Novick ◽  
...  
Keyword(s):  
Cation Exchange ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Igg2 Antibody

Characterization of the Acidic Species of a Monoclonal Antibody Using Weak Cation Exchange Chromatography and LC-MS

2015 ◽  
Vol 87 (17) ◽  
pp. 9084-9092 ◽  
Author(s):  
Gomathinayagam Ponniah ◽  
Adriana Kita ◽  
Christine Nowak ◽  
Alyssa Neill ◽  
Yekaterina Kori ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cation Exchange ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Weak Cation Exchange

Chromatographic separations of diastereoisomers of the 5,7,12,14-tetramethyl-1,4,8,11 -tetraazacyclotetradeca-4,11-dienenickel(II) cation, and characterization of the nickel(II) complex of the new macrocyclic ligand 5,7,7,12,14-pentamethyl- 1,4,8,11 -tetraazacyclotetradeca-4,11-diene

1980 ◽  
Vol 33 (7) ◽  
pp. 1447 ◽  
Author(s):  
GH Searle ◽  
RW Hay ◽  
B Jeragh
Keyword(s):  
Cation Exchange ◽  
Column Chromatography ◽  
Macrocyclic Ligand ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Chromatographic Separations ◽  
Chiral Carbon ◽  
Diene Ligands

Planar nickel(II) complexes of different macrocyclic tetraazacyclotetradeca-4,11-diene ligands and diastereoisomers which differ in chiral carbon configurations have been separated by cation- exchange chromatography on SP-Sephadex C-25 resin. Of the limited number of successful eluents, sodium glycinate is the most effective. The separations are considered to result from differential associations between the glycinate anion and the complex cations. The technique has been successfully applied to the separation of the C-racemic and C-meso diastereoisomers of [Ni(ttcd)]2+ (ttcd = 5,7,12,14-tetramethyl-1,4,8,11- tetraazacyclotetradeca-4,11-diene). Diastereoisomers arising from chiral NH centres cannot be separated by this technique since the effective eluents are generally basic, causing epimerization. The use of 1H and 13C n.m.r. spectra in these studies is discussed, and the isolation of the nickel(II) complex of the new macrocyclic ligand ptcd (5,7,7,12,14-pentamethyl-1,4,8,11-tetraazacyclotetradeca-4,11-diene) by column chromatography described. The isolated [Ni(ptcd)]-(ClO4)2 is a mixture of the diastereoisomers C-axial,N-racemic and C-axial,N-meso.

scholarly journals Application of High-performance Cation-exchange Chromatography for Characterization of Stinging Insect Venoms.

1983 ◽  
Vol 37b ◽  
pp. 252-254 ◽  
Author(s):  
Roland Einarsson ◽  
Ing-Mari Åstrand ◽  
Per Ålin ◽  
Bengt Mannervik ◽  
Jan-Eric Berg ◽  
...  
Keyword(s):  
Cation Exchange ◽  
High Performance ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Stinging Insect

Characterization of asparagine 330 deamidation in an Fc-fragment of IgG1 using cation exchange chromatography and peptide mapping

2014 ◽  
Vol 965 ◽  
pp. 65-71 ◽  
Author(s):  
Yonghua Taylor Zhang ◽  
Jennifer Hu ◽  
Amanda L. Pace ◽  
Rita Wong ◽  
Y. John Wang ◽  
...  
Keyword(s):  
Cation Exchange ◽  
Peptide Mapping ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Fc Fragment

scholarly journals Characterization of therapeutic proteins by cation exchange chromatography-mass spectrometry and top-down analysis

mAbs ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 1739825 ◽  
Author(s):  
Rachel Liuqing Shi ◽  
Gang Xiao ◽  
Thomas M. Dillon ◽  
Margaret S. Ricci ◽  
Pavel V. Bondarenko
Keyword(s):  
Mass Spectrometry ◽  
Cation Exchange ◽  
Therapeutic Proteins ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Top Down ◽  
Chromatography Mass Spectrometry

Purification and Characterization of a β-Glucosidase Specific for 2,4-Dihydroxy- 7-methoxy-1,4-benzoxazin-3-one (DIMBOA) Glucoside in Maize

1999 ◽  
Vol 54 (3-4) ◽  
pp. 181-185 ◽  
Author(s):  
Akira Oikawa ◽  
Kenkichi Ebisui ◽  
Masayuki Sue ◽  
Atsushi Ishihara ◽  
Hajime Iwamura
Keyword(s):  
Zea Mays ◽  
Ion Exchange ◽  
Cation Exchange ◽  
Hydroxamic Acid ◽  
Zea Mays L ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Purification And Characterization ◽  
Apparent Homogeneity

Occurrence and properties of hydroxamic acid glucoside glucosidase were investigated in 10-day-old, autotrophic maize (Zea mays L.) in which 2,4-dihydroxy-7-methoxy-1,4- benzoxazin-3-one glucoside (DIMBOA-G) is a major benzoxazinone component. Crude extracts of both leaves and roots showed glucosidase activity for both DIMBOA-G and 2,4- dihydroxy-1,4-benzoxazin-3-one glucoside (DIBOA-G). A cation-exchange chromatography after cryoprecipitation of the extract from leaves gave a peak with both activities, and further purification by ion-exchange and hydroxyapatite chromatography gave a fraction with an apparent homogeneity, the purification being 560 fold. The Km values (mᴍ) of the purified glucosidase were 0.16 for DIMBOA-G, 0.68 for DIBOA-G and 2.96 for p-nitrophenyl-β-ᴅ-glucopyranoside. The activity on salicin and esculin was too low to be detected. The data indicate that a glucosidase specific for DIMBOA-G comes into contact with constitutive benzoxazinone glucosides producing defensive aglycone when plants are damaged by microbial or insect attacks.

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