A Method for Differentiating Fetal Bovine Serum from Newborn Calf Serum

2017 ◽  
Vol 16 ◽  
Author(s):  
Michelle Cheever ◽  
Rosemary Versteegen ◽  
Alyssa Master
Keyword(s):  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Calf Serum ◽  
Newborn Calf Serum ◽  
Fetal Bovine

A comparison of the effects of fetal bovine serum and newborn calf serum on cell growth and maintenance of cryopreserved mouse spermatogonial stem cells

2020 ◽  
Vol 47 (12) ◽  
pp. 9609-9614
Author(s):  
Afshin Pirnia ◽  
Vahideh Assadollahi ◽  
Masoud Alasvand ◽  
Arezou Moghadam ◽  
Mohammad Reza Gholami
Keyword(s):  
Stem Cells ◽  
Cell Growth ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Calf Serum ◽  
Spermatogonial Stem Cells ◽  
Newborn Calf Serum ◽  
Fetal Bovine

Effects of Resin or Charcoal Treatment on Fetal Bovine Serum and Bovine Calf Serum

2009 ◽  
Vol 34 (4) ◽  
pp. 101-108 ◽  
Author(s):  
Zhimin Cao ◽  
Clint West ◽  
Carol S. Norton-Wenzel ◽  
Robert Rej ◽  
Faith B. Davis ◽  
...  
Keyword(s):  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Calf Serum ◽  
Bovine Calf Serum ◽  
Fetal Bovine

scholarly journals Study on the growth promoting capacity of calf and fetal bovine serum for animal cells "in vitro" II: electrophoretic study and survey on the antiproteolytic activity of pools of calf and fetal bovine serum

1984 ◽  
Vol 26 (2) ◽  
pp. 97-104 ◽  
Author(s):  
Edda de Rizzo ◽  
Celidéia Aparecida Coppi Vaz ◽  
Inácio França Mendes ◽  
Anatércia Ferreira Bonfim Yano
Keyword(s):  
Bovine Serum ◽  
Culture Media ◽  
Fetal Bovine Serum ◽  
Calf Serum ◽  
Animal Origin ◽  
Electrophoretic Study ◽  
Fetal Bovine ◽  
Growth Promoting ◽  
Antiproteolytic Activity

Calf serum and fetal bovine serum present great variability as to its growth promoting efficiency (GPE). As supplement of culture media to cultivate cells of animal origin they stimulate the "in vitro" multiplication and maintain cell viability. When fourteen lots of calf sera of variable GPE had the total protein contents as well as the percentages of serum fractions determined, no significant differences that could possibly explain the variability of the GPE were observed. Evaluation of the antiproteolytic activity of nineteen lots of calf serum and eighteen serum lots of younger calves showed that the former exhibited lower antiproteolytic titers (1:40 to 1:80) than the latter (1:80 to 1:160). Twelve lots of fetal bovine serum studied in parallel, showed the highest concentration of antiproteolytic factors, with titers equal to 1:320. Sera of bovine origin, but not fetal sera, are usually heat-inactivated, what was demonstrated to be responsible for the decrease of the antiproteolytic activity of 75% of the lots tested. This could explain the inability of certain heat-inactivated sera in promoting multiplication of some cells "in vitro", as verified with primary monkey kidney cells. The results obtained in this study indicated the convenience of submiting each lot of serum to be introduced in cell culture to previous determination of its characteristics, such as growth promoting efficiency, antiproteolytic activity and also toxicity, absence of extraneous agents, etc., in order to minimize the possibility of using serum lots of questionable quality, thus preventing not only the loss of cell lines, but also undesirable and sometimes expensive delays.

117 EFFECT OF STEPWISE DILUTION ON THE VIABILITY OF FROZEN - THAWED BOVINE OOCYTES MATURED IN VITRO

2007 ◽  
Vol 19 (1) ◽  
pp. 176 ◽  
Author(s):  
A. Hamawaki ◽  
S. Hamano ◽  
M. Yoshikawa ◽  
K. Matsukawa
Keyword(s):  
Bovine Serum ◽  
Room Temperature ◽  
Fetal Bovine Serum ◽  
Calf Serum ◽  
Fertilization Rate ◽  
Single Step ◽  
Normal Morphology ◽  
Fetal Bovine ◽  
Bovine Oocytes

The purpose of this study was to evaluate the effect of stepwise dilution on the viability of frozen–thawed bovine oocytes matured in vitro. Oocytes matured in vitro were denuded and equilibrated in modified TCM-199 (m199: 11 mmol L-1 HEPES, 9 mmol L-1 Na-HEPES, 5 mmol L-1 NaHCO3, 20% (v/v) calf serum) supplemented with 10% (v/v) glycerol for 15 min at room temperature (RT). Then they were exposed to m199 with 10% glycerol and 0.25 mol L-1 sucrose and loaded into 0.25-mL plastic straws. The straws were sealed and seeded at -6�C, cooled at the rate of 0.33�C min-1 to -25�C, and plunged into LN2. For thawing, the straws were first held in air at RT for 10 s, followed by immersion in 30�C water for 10 s. In the first experiment, frozen-thawed oocytes were subjected to cryoprotectants in 5 different manners of dilution. In the non-step dilution, the oocytes (n = 60) were put into m199 for 5 min. In the single-step dilution, the oocytes (n = 37) were transferred to 0.25 mol L-1 sucrose in m199 for 5 min. In the two-step dilution, the oocytes (n = 56) were transferred to 0.5 and then 0.25 mol L-1 sucrose in m199 for 5 and 5 min, respectively. In the three-step dilution, the oocytes (n = 57) were transferred to 0.75, 0.5, and 0.25 mol L-1 sucrose in m199 for 1, 5, and 5 min, respectively. In the four-step dilution, the oocytes (n = 52) were transferred to 1.0, 0.75, 0.5, and 0.25 mol L-1 sucrose in m199 for 1, 1, 5, and 5 min, respectively. After dilution, all of the oocytes were washed twice in TCM-199 supplemented with 5% fetal bovine serum for 5 min and cultured for 1 h to assess the morphology. The rate of morphological normal oocytes in the four-step dilution (94.2%) was significantly (P < 0.05) higher than that in other groups (non-, single-, two-, and three-step dilution: 61.7%, 73.0%, 78.6%, and 77.2%). In the second experiment, non-frozen (control, n = 170) and frozen–thawed oocytes (n = 145) with four-step dilution were fertilized and cultured in vitro (Kuwayama 1992 J. Reprod. Fert. 96, 187–193). To assess fertilization, some of the oocytes were fixed at 10 h after insemination. Cleavage and blastocyst rates were determined on Day 2 and Day 8 after fertilization (Day 0), respectively. There was no difference (P > 0.05) between control and frozen–thawed oocytes in the fertilization rate (88.0% vs. 93.1%). Some of the frozen–thawed oocytes cleaved and developed to blastocysts (44.0% and 11.2%), although the rates were significantly (P < 0.01) lower than those in control (71.7% and 35.0%). These results indicate that stepwise dilution of frozen–thawed oocytes improves the recovery of oocytes with normal morphology, and that the oocytes maintain the abilities to be fertilized and develop to blastocysts.

Treatment of fetal bovine serum improves early development of porcine embryos by alleviating oxidative stress

2014 ◽  
Author(s):  
Seon-A Choi ◽  
Seong-Eun Mun ◽  
Pil-Soo Jeong ◽  
Hae-Jun Yang ◽  
Seung-Bin Yoon ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Early Development ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

МОДЕЛИРОВАНИЕ СИСТЕМ ЭКСТРАКОРПОРАЛЬНОГО СОЗРЕВАНИЯ ООЦИТОВ КРУПНОГО РОГАТОГО СКОТА С ИСПОЛЬЗОВАНИЕМ СТРУКТУРНЫХ КОМПОНЕНТОВ ОВАРИАЛЬНЫХ ФОЛЛИКУЛОВ *

2019 ◽  
pp. 20-22
Author(s):  
T.I. KUZMINA ◽  
I.V. CHISTYAKOVA
Keyword(s):  
Granulosa Cells ◽  
Follicular Fluid ◽  
Bovine Serum ◽  
Egg Quality ◽  
Fetal Bovine Serum ◽  
Reproductive Technologies ◽  
Blastocyst Stage ◽  
Preimplantation Embryos ◽  
Cultural Systems ◽  
Fetal Bovine

Создание эффективной унифицированной системы дозревания донорских ооцитов обеспечит повышение результативности инновационных клеточных репродуктивных технологий. В исследовании проведен сравнительный мониторинг показателеймейотического созревания ооцитов коров, созревших в различных системах, дополненных структурными компонентами фолликулов (СКФ стенки фолликулов, клетки гранулезы, белки) и фолликулярной жидкостью,а также потенций к развитию из них доимплантационных эмбрионов. Анализу подверглись ооциты, прокультивированные в следующих системах:среда ТС199 с добавлением 10 фетальной бычьей сыворотки (ФБС), 50 мкг/мл эстрадиола, 10 мкг/мл лютеинизирующего гормона (ЛГ), 10 мкг/мл фолликулостимулирующего гормона (ФСГ) среда ТС199 с 10 эстральной сывороткой коров среда ТС199 с 50 жидкости из фолликулов диаметром 9 мм среда ТС199 с добавлением белков фолликулярной жидкости молекулярной массой 65 кДасреда ТС199 с 10 ФБС и 1106 клеток гранулезы среда ТС199 с 10 ФБС и тканью фолликула. В культуральные среды ко всем исследованным группам ооцитов добавляли антибиотики. Использование CКФ обеспечило значительное снижение доли ооцитов с дегенерированным хроматином, что способствовало увеличению уровня доимпланационных эмбрионов на стадии бластоцисты. Так, доля бластоцист, развившихся из ооцитов, созревших в среде со стенками фолликулов,составила43,5. В этой же группе выявлен минимальный уровень дегенерированных зародышей (6,45). Полученные данные предлагается использовать при моделировании систем дозревания ооцитов коров с целью повышения качества яйцеклеток.The creation of an effective unified maturation system of donor oocytes provides an increase in the efficiency of innovative cellular reproductive technologies. The comparative analysis of the meiotic maturation indicators of bovine oocytes, which were matured in different cultural systems modified by follicular structural components (FSC follicular walls, granulosa cells, proteins) and follicular fluid, as well as the potential for preimplantation embryonic development were evaluated in this study. Oocytes matured in following cultural systems: medium TC199 supplemented with 10 fetal bovine serum and 50 g/ml of estradiol, 10 g/ml of luteinizing hormone (LH), 10 g/ml of folliclestimulating hormone (FSH) medium TC199 with 10 estrous cow serum medium TC199 with 50 liquid from follicles with a diameter of 9 mm medium TC199 supplemented with the follicular fluid proteins with molecular weight 65 kDa medium TC199 with 10 fetal bovine serum and 1106 granulosa cells medium TC199 with the addition of 10 fetal bovine serum and follicle tissues were analyzed. Antibiotics were added to cultural media of all experimental groups of oocytes. The usage of FSC ensured the decrease in the proportion of oocytes with degenerated chromatin, which contribute the rise of the level of preimplantation embryos at the blastocyst stage. Thus, the proportion of blastocysts developed from oocytes matured in medium supplemented with follicular walls was 43.5. In the same experimental group, the number of degenerated embryos was 6.45. The obtained data are supposed to be used for modeling the cultural systems of cow oocytes in order to improve the egg quality.

scholarly journals Effect of fetal bovine serum on erythropoietin receptor expression and viability of breast cancer cells

2020 ◽  
Vol 27 (2) ◽  
pp. 653-658
Author(s):  
Guan-Young Teo ◽  
Abdullah Rasedee ◽  
Nagi. A. AL-Haj ◽  
Chaw Yee Beh ◽  
Chee Wun How ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Bovine Serum ◽  
Breast Cancer Cells ◽  
Fetal Bovine Serum ◽  
Erythropoietin Receptor ◽  
Receptor Expression ◽  
Fetal Bovine
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