scholarly journals A Simple Method for Optimization of Reference Gene Identification and Normalization in DNA Microarray Analysis

2016 ◽  
Vol 22 ◽  
pp. 45-52 ◽  
Author(s):  
Federico M. Casares
Keyword(s):  
Microarray Analysis ◽  
Reference Gene ◽  
Dna Microarray ◽  
Gene Identification ◽  
Simple Method ◽  
Dna Microarray Analysis

scholarly journals The Potential of 2-aza-8-Oxohypoxanthine as a Cosmetic Ingredient

Cosmetics ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 60
Author(s):  
Hisae Aoshima ◽  
Masayuki Ito ◽  
Rinta Ibuki ◽  
Hirokazu Kawagishi
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Cell Activation ◽  
Skin Barrier ◽  
Efficacy Evaluation ◽  
Activation Effect ◽  
Expression Levels ◽  
Dna Microarray Analysis

In this study, we verified the effects of 2-aza-8-oxohypoxanthine (AOH) on human epidermal cell proliferation by performing DNA microarray analysis. Cell proliferation was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, which measures mitochondrial respiration in normal human epidermal keratinocyte (NHEK) cells. Gene expression levels were determined by DNA microarray analysis of 177 genes involved in skin aging and disease. AOH showed a significant increase in cell viability at concentrations between 7.8 and 31.3 μg/mL and a significant decrease at concentrations above 250 μg/mL. DNA microarray analysis showed that AOH significantly increased the gene expression of CLDN1, DSC1, DSG1, and CDH1 (E-cadherin), which are involved in intercellular adhesion and skin barrier functioning. AOH also up-regulated the expression of KLK5, KLK7, and SPIMK5, which are proteases involved in stratum corneum detachment. Furthermore, AOH significantly stimulated the expression of KRT1, KRT10, TGM1, and IVL, which are considered general differentiation indicators, and that of SPRR1B, a cornified envelope component protein. AOH exerted a cell activation effect on human epidermal cells. Since AOH did not cause cytotoxicity, it was considered that the compound had no adverse effects on the skin. In addition, it was found that AOH stimulated the expression levels of genes involved in skin barrier functioning by DNA microarray analysis. Therefore, AOH has the potential for practical use as a cosmetic ingredient. This is the first report of efficacy evaluation tests performed for AOH.

scholarly journals DNA Microarray Analysis Identifies CKS2 and LEPR as Potential Markers of Meningioma Recurrence

2011 ◽  
Vol 16 (10) ◽  
pp. 1440-1450 ◽  
Author(s):  
Francesca Menghi ◽  
Francesca N. Orzan ◽  
Marica Eoli ◽  
Mariangela Farinotti ◽  
Emanuela Maderna ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Dna Microarray ◽  
Dna Microarray Analysis ◽  
Meningioma Recurrence

scholarly journals DNA microarray analysis of global gene regulation by A-factor in Streptomyces griseus

Microbiology ◽  
2009 ◽  
Vol 155 (7) ◽  
pp. 2197-2210 ◽  
Author(s):  
Hirofumi Hara ◽  
Yasuo Ohnishi ◽  
Sueharu Horinouchi
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Streptomyces Griseus ◽  
Exponential Growth Phase ◽  
Mobility Shift ◽  
Dna Microarray Analysis ◽  
Regulatory Cascade ◽  
Transcriptional Units ◽  
Inducible Genes

A-factor (2-isocapryloyl-3R-hydroxymethyl-γ-butyrolactone) is a microbial hormone that triggers morphological differentiation and secondary metabolism in Streptomyces griseus. The effects of A-factor on global gene expression were determined by DNA microarray analysis of transcriptomes obtained with the A-factor-deficient mutant ΔafsA. A-factor was added at a concentration of 25 ng ml−1 to mutant ΔafsA at the middle of the exponential growth phase, and RNA samples were prepared from the cells grown after A-factor addition for a further 5, 15 and 30 min, and 1, 2, 4, 8 and 12 h. The effects of A-factor on transcription of all protein-coding genes of S. griseus were evaluated by comparison of the transcriptomes with those obtained from cells grown in the absence of A-factor. Analysis of variance among the transcriptomes revealed that 477 genes, which were dispersed throughout the chromosome, were differentially expressed during the 12 h after addition of A-factor, when evaluated by specific criteria. Quality threshold clustering analysis with regard to putative polycistronic transcriptional units and levels of upregulation predicted that 152 genes belonging to 74 transcriptional units were probable A-factor-inducible genes. Competitive electrophoretic mobility shift assays using DNA fragments including putative promoter regions of these 74 transcriptional units suggested that AdpA bound 37 regions to activate 72 genes in total. Many of these A-factor-inducible genes encoded proteins of unknown function, suggesting that the A-factor regulatory cascade of S. griseus affects gene expression at a specific time point more profoundly than expected.

scholarly journals DNA microarray analysis of normal rat kidney epithelial cells treated with cadmium

2011 ◽  
Vol 36 (1) ◽  
pp. 127-129 ◽  
Author(s):  
Maki Tokumoto ◽  
Tomoaki Ohtsu ◽  
Akiko Honda ◽  
Yasuyuki Fujiwara ◽  
Hisamitsu Nagase ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Rat Kidney ◽  
Dna Microarray Analysis ◽  
Normal Rat ◽  
Kidney Epithelial Cells
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