scholarly journals Resveratrol protects vascular smooth muscle cells against high glucose-induced oxidative stress and cell proliferation in vitro

2014 ◽  
Vol 20 ◽  
pp. 82-92 ◽  
Author(s):  
Yawei Xu
Keyword(s):  
Oxidative Stress ◽  
Cell Proliferation ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
High Glucose ◽  
Muscle Cells ◽  
Proliferation In Vitro

Wheat germ-derived peptide ADWGGPLPH abolishes high glucose-induced oxidative stress via modulation of the PKCζ/AMPK/NOX4 pathway

2020 ◽  
Vol 11 (8) ◽  
pp. 6843-6854 ◽  
Author(s):  
Fang Wang ◽  
Zebin Weng ◽  
Yi Lyu ◽  
Yifan Bao ◽  
Juncheng Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
High Glucose ◽  
Wheat Germ ◽  
Muscle Cells ◽  
Antioxidative Effect ◽  
Underlying Mechanisms

This study explores the antioxidative effect of a specific wheat germ-derived peptide on high glucose-induced oxidative stress in vascular smooth muscle cells (VSMCs) and the underlying mechanisms.

scholarly journals ADAMTS-7 promotes vascular smooth muscle cells proliferation in vitro and in vivo

2015 ◽  
Vol 58 (7) ◽  
pp. 674-681 ◽  
Author(s):  
Lu Zhang ◽  
Fang Yu ◽  
Li Wang ◽  
JinGang Zheng ◽  
YaoYao Du ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Proliferation In Vitro

Abstract 462: Ascl3 Protects Vascular Smooth Muscle Cells Against Oxidative Stress

2013 ◽  
Vol 33 (suppl_1) ◽  
Author(s):  
Swarajit K Biswas ◽  
Liliana Hernandez ◽  
Yan Xiao ◽  
Mushtaq Ahmad ◽  
Minerva T Garcia-Barrio
Keyword(s):  
Oxidative Stress ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Mitochondrial Pathway ◽  
Protective Role ◽  
Over Expression

Atherosclerosis initiation and progression depends on multiple risk factors and is sustained by self-perpetuating inflammatory cellular and molecular events that ultimately translate into increased oxidative stress inducing endothelial dysfunction, macrophage activation and altering vascular smooth muscle cell (VSMC) homeostasis. Our initial work identified the under-characterized bHLH achaete-scute like 3, ASCL3, as a pro-proliferative anti-apoptotic factor in VSMC and prompted the hypothesis that ASCL3 plays a protective role against oxidative stress in VSMC. We determined that ASCL3 expression is up-regulated in the media and lesions of the atherosclerotic aorta in ApoE-mice fed a high fat diet. In vitro , ASCL3 expression is consistent with an adaptive role in oxidative stress. In primary RASMC, time- and dose-response to H 2 O 2 (0.5-2mM, 4-24h) result in marked ASCL3 mRNA increase (~10-fold) at low concentrations or shorter times and is reduced as oxidative stress increases. siRNA knockdown of ASCL3 increases susceptibility of RASMC to 0.25mM H 2 O 2 (low dose) as determined by caspase-3 activation. Stable over-expression of ASCL3 in A7r5 cells results in enhanced protection at higher doses of H 2 O 2 , and is associated with higher AKT and ERK1/2 phosporylation. This protective effect involves the mitochondrial pathway since ASCL3 up-regulates Bcl-2/Bax ratio in basal and induced conditions (0.4mM, 4h), inhibits mitochondrial depolarization, prevents mitochondrial fragmentation and preserves mitochondrial DNA. ROS levels are significantly reduced in ASCL3 over-expressing cells (0.2mM, 2h). ASCL3 over-expression appears to increase mitochondrial density and results in altered mitochondrial and cristae morphology as determined by TEM. Surprisingly, subcellular localization studies indicate that ASCL3 localizes primarily to the mitochondria. In summary, low oxidative stress in VSMC promotes survival, with enhanced proliferation, and as it increases, induces adaptation associated with an antioxidant gene expression profile. Eventually high oxidative stress leads to cell death. Altogether, our data indicate that ASCL3 plays a protective role in the survival and adaptation of vascular smooth muscle cells to oxidative stress.

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