scholarly journals Concentrated Growth Factor Promotes Dental Pulp Cells Proliferation and Mineralization and Facilitates Recovery of Dental Pulp Tissue

2019 ◽  
Vol 25 ◽  
pp. 10016-10028 ◽  
Author(s):  
Songbo Tian ◽  
Jie Wang ◽  
Fusheng Dong ◽  
Nan Du ◽  
Wenjing Li ◽  
...  
Keyword(s):  
Growth Factor ◽  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Pulp Tissue ◽  
Dental Pulp Tissue ◽  
Pulp Cells

Regeneration of dental pulp tissue in immature teeth with apical periodontitis using platelet-rich plasma and dental pulp cells

2013 ◽  
Vol 46 (10) ◽  
pp. 962-970 ◽  
Author(s):  
W. Zhu ◽  
X. Zhu ◽  
G. T.-J. Huang ◽  
G. S. P. Cheung ◽  
W. L. Dissanayaka ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Platelet Rich Plasma ◽  
Apical Periodontitis ◽  
Dental Pulp Cells ◽  
Pulp Tissue ◽  
Immature Teeth ◽  
Dental Pulp Tissue ◽  
Pulp Cells

scholarly journals Biological Effects of Amelogenin Exon 5 Encoded Peptide from Enamel Matrix Derivative in Human Dental Pulp Cells

2018 ◽  
Author(s):  
Hirohito Kato ◽  
Yoichiro Taguchi ◽  
Kazuya Tominaga ◽  
Masahiro Noguchi ◽  
Kazutaka Imai ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Dental Pulp ◽  
Mapk Signaling ◽  
Mapk Signaling Pathway ◽  
Enamel Matrix Derivative ◽  
Dental Pulp Cells ◽  
Pulp Tissue ◽  
Dental Pulp Tissue ◽  
Pulp Cells ◽  
Matrix Derivative

Enamel matrix derivative (EMD) is used for periodontal tissue regeneration therapy, and can induce mineralization in dental pulp cells (DPCs). We designed a synthetic peptide (SP) derived from the response of cells to EMD, and investigated the effect of the SP on potentiating osteogenesis in DPCs, which have a critical role of dental pulp homeostasis. DPCs were treated with 0, 10, 100, or 1000 ng/mL SP to determine its effect on cell proliferation, cell migration, cell differentiation, and mineralization. We then examined the molecular effects of the SP, focusing on changes in the mitogen-activated protein kinases (MAPK) signaling pathway in these cells. The SP significantly promoted DPC proliferation and migration. Cultures treated with the SP also showed an enhanced expression of markers of osteogenic differentiation and mineralization. The SP also induced the activation of MAPK signaling pathway components. These results suggest that our SP could promote the dental pulp tissue repair by hard tissue formation and the mineralization through activating MAPK signaling pathway. This study provides the first evidence that SP might be a new material for dental pulp tissue treatment.

scholarly journals Effects of Papain-Based Gel Used For Caries Removal on Macrophages and Dental Pulp Cells

2019 ◽  
Vol 30 (5) ◽  
pp. 484-490
Author(s):  
Laura Alves Bastos ◽  
Francine Lorencetti Silva ◽  
João Pedro de Queiroz Thomé ◽  
Maya Fernanda Manfrin Arnez ◽  
Lúcia Helena Faccioli ◽  
...  
Keyword(s):  
Cell Viability ◽  
Dental Pulp ◽  
Colorimetric Assay ◽  
Inhibitory Effect ◽  
Dental Pulp Cells ◽  
Pulp Tissue ◽  
Dental Pulp Tissue ◽  
Pulp Cells ◽  
Caries Removal ◽  
Pre Treatment

Abstract Papain-based gel is used for chemical-mechanical caries removal and present antimicrobial and anti-inflammatory activities. However, its effects on dental pulp cells and on macrophages remains largely unknown. Therefore, the aim of this study was to investigate whether the papain-based gel Papacárie Duo® acts as an immunomodulator in lipopolysaccharide (LPS)-activated macrophages and its effects on dental pulp cells . J774.1 macrophage and OD-21 dental pulp cells were stimulated with 0.5% and 5% of Papacárie Duo®, following pre-treatment or not with LPS. After 24 h, a lactate dehydrogenase assay was used to measure cytotoxicity, a tetrazolium-based colorimetric assay (MTT) was used to measure cell viability, and qRT-PCR was used to analyze relative gene expression of Ptgs2, Il10, Tnf, Mmp9, Runx2, Ibsp and Spp1. Papacárie Duo® was cytotoxic and reduced cell viability at 5% but not at 0.5% in both cultures. In macrophages, Papacárie Duo® increased the expression Il10 and LPS-induced Ptgs2, but it did not affect Tnf or Mmp9. In OD-21 cells, Papacárie Duo® inhibited Runx2 and Ibsp expression, but stimulated Spp1 expression. Papain-based gel presented a concentration dependent cytotoxicity, without affecting cell viability, for dental pulp cells and macrophages. Interestingly, the gel presented an inhibitory effect on pulp cell differentiation but modulated the activation of macrophages stimulated with LPS. We speculate that in dental pulp tissue, Papacárie Duo® would impair reparative dentinogenesis but could activate macrophages to perform their role in defense and inflammation.

Melatonin levels in human diabetic dental pulp tissue and its effects on dental pulp cells under hyperglycaemic conditions

2018 ◽  
Vol 51 (10) ◽  
pp. 1149-1158 ◽  
Author(s):  
A. Milosavljević ◽  
Lj. DJukić ◽  
B. Toljić ◽  
J. Milašin ◽  
B. DŽeletović ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Dental Pulp Cells ◽  
Pulp Tissue ◽  
Dental Pulp Tissue ◽  
Pulp Cells

Effects of Recombinant Dentin Sialoprotein in Dental Pulp Cells

2012 ◽  
Vol 91 (4) ◽  
pp. 407-412 ◽  
Author(s):  
S.-Y. Lee ◽  
S.-Y. Kim ◽  
S.-H. Park ◽  
J.-J. Kim ◽  
J.-H. Jang ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Nuclear Translocation ◽  
Nodule Formation ◽  
Dental Pulp Cells ◽  
Pulp Tissue ◽  
Human Dental Pulp Cells ◽  
Exogenous Addition ◽  
Pulp Cells ◽  
Dentin Sialoprotein ◽  
And Migration

Dentin sialophosphoprotein (DSPP) is critical for dentin mineralization. However, the function of dentin sialoprotein (DSP), the cleaved product of DSPP, remains unclear. This study aimed to investigate the signal transduction pathways and effects of recombinant human DSP (rh-DSP) on proliferation, migration, and odontoblastic differentiation in human dental pulp cells (HDPCs). The exogenous addition of rh-DSP enhanced the proliferation and migration of HDPCs in dose- and time-dependent manners. rh-DSP markedly increased ALP activity, calcium nodule formation, and levels of odontoblastic marker mRNA. rh-DSP increased BMP-2 expression and Smad1/5/8 phosphorylation, which was blocked by the BMP antagonist, noggin. Furthermore, rh-DSP phosphorylated extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), Akt, and IκB-α, and induced the nuclear translocation of the NF-κB p65 subunit. Analysis of these data demonstrates a novel signaling function of rh-DSP for the promotion of growth, migration, and differentiation in HDPCS via the BMP/Smad, JNK, ERK, MAPK, and NF-κB signaling pathways, suggesting that rh-DSP may have therapeutic utility in dentin regeneration or dental pulp tissue engineering.

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