scholarly journals Fluid Shear Stress Suppresses Osteoclast Differentiation in RAW264.7 Cells through Extracellular Signal-Regulated Kinase 5 (ERK5) Signaling Pathway

2020 ◽  
Vol 26 ◽  
Author(s):  
Chongwen Ma ◽  
Bin Geng ◽  
Xiaohui Zhang ◽  
Rui Li ◽  
Xinxin Yang ◽  
...  
Keyword(s):  
Shear Stress ◽  
Signaling Pathway ◽  
Fluid Shear Stress ◽  
Osteoclast Differentiation ◽  
Raw264.7 Cells ◽  
Fluid Shear ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal

scholarly journals Flow-induced Shear Stress Confers Resistance to Carboplatin in an Adherent Three-Dimensional Model for Ovarian Cancer: A Role for EGFR-Targeted Photoimmunotherapy Informed by Physical Stress

2020 ◽  
Vol 9 (4) ◽  
pp. 924
Author(s):  
Shubhankar Nath ◽  
Michael Pigula ◽  
Amjad P. Khan ◽  
William Hanna ◽  
Mustafa Kemal Ruhi ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Shear Stress ◽  
Treatment Failure ◽  
Fluid Shear Stress ◽  
Physical Stress ◽  
Molecular Heterogeneity ◽  
Fluid Shear ◽  
Extracellular Signal Regulated Kinase ◽  
Extracellular Signal ◽  
Static Conditions

A key reason for the persistently grim statistics associated with metastatic ovarian cancer is resistance to conventional agents, including platinum-based chemotherapies. A major source of treatment failure is the high degree of genetic and molecular heterogeneity, which results from significant underlying genomic instability, as well as stromal and physical cues in the microenvironment. Ovarian cancer commonly disseminates via transcoelomic routes to distant sites, which is associated with the frequent production of malignant ascites, as well as the poorest prognosis. In addition to providing a cell and protein-rich environment for cancer growth and progression, ascitic fluid also confers physical stress on tumors. An understudied area in ovarian cancer research is the impact of fluid shear stress on treatment failure. Here, we investigate the effect of fluid shear stress on response to platinum-based chemotherapy and the modulation of molecular pathways associated with aggressive disease in a perfusion model for adherent 3D ovarian cancer nodules. Resistance to carboplatin is observed under flow with a concomitant increase in the expression and activation of the epidermal growth factor receptor (EGFR) as well as downstream signaling members mitogen-activated protein kinase/extracellular signal-regulated kinase (MEK) and extracellular signal-regulated kinase (ERK). The uptake of platinum by the 3D ovarian cancer nodules was significantly higher in flow cultures compared to static cultures. A downregulation of phospho-focal adhesion kinase (p-FAK), vinculin, and phospho-paxillin was observed following carboplatin treatment in both flow and static cultures. Interestingly, low-dose anti-EGFR photoimmunotherapy (PIT), a targeted photochemical modality, was found to be equally effective in ovarian tumors grown under flow and static conditions. These findings highlight the need to further develop PIT-based combinations that target the EGFR, and sensitize ovarian cancers to chemotherapy in the context of flow-induced shear stress.

Cyclic fluid shear stress promotes osteoblastic cells proliferation through ERK5 signaling pathway

2012 ◽  
Vol 364 (1-2) ◽  
pp. 321-327 ◽  
Author(s):  
Peng Li ◽  
Yan-chao Ma ◽  
Xiao-yun Sheng ◽  
Hai-tao Dong ◽  
Hua Han ◽  
...  
Keyword(s):  
Shear Stress ◽  
Signaling Pathway ◽  
Fluid Shear Stress ◽  
Osteoblastic Cells ◽  
Fluid Shear

P 7 Fluid shear stress promotes placental growth factor upregulation in human syncytiotrophoblast through the camp-pka signaling pathway

2017 ◽  
Vol 9 ◽  
pp. 39-40
Author(s):  
Edouard Lecarpentier ◽  
Anthony Atallah ◽  
Jean Guibourdenche ◽  
Marylise Hebert-Schuster ◽  
Bassam Haddad ◽  
...  
Keyword(s):  
Shear Stress ◽  
Growth Factor ◽  
Signaling Pathway ◽  
Fluid Shear Stress ◽  
Placental Growth Factor ◽  
Fluid Shear

scholarly journals Fluid Shear Stress Promotes Placental Growth Factor Upregulation in Human Syncytiotrophoblast Through the cAMP–PKA Signaling Pathway

Hypertension ◽  
2016 ◽  
Vol 68 (6) ◽  
pp. 1438-1446 ◽  
Author(s):  
Edouard Lecarpentier ◽  
Anthony Atallah ◽  
Jean Guibourdenche ◽  
Marylise Hebert-Schuster ◽  
Sarah Vieillefosse ◽  
...  
Keyword(s):  
Shear Stress ◽  
Growth Factor ◽  
Signaling Pathway ◽  
Fluid Shear Stress ◽  
Placental Growth Factor ◽  
Fluid Shear

Fluid shear stress promotes osteoblast proliferation via the Gαq–ERK5 signaling pathway

2016 ◽  
Vol 57 (4) ◽  
pp. 299-306 ◽  
Author(s):  
Zhang Bo ◽  
Geng Bin ◽  
Wang Jing ◽  
Wang Cuifang ◽  
An Liping ◽  
...  
Keyword(s):  
Shear Stress ◽  
Signaling Pathway ◽  
Fluid Shear Stress ◽  
Osteoblast Proliferation ◽  
Fluid Shear
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