scholarly journals A Nude Mouse Model of Orthotopic Liver Transplantation of Human Hepatocellular Carcinoma HCCLM3 Cell Xenografts and the Use of Imaging to Evaluate Tumor Progression

2019 ◽  
Vol 25 ◽  
pp. 8694-8703 ◽  
Author(s):  
Zhi-ting Xu ◽  
Hong Ding ◽  
Tian-tian Fu ◽  
Yu-li Zhu ◽  
Wen-ping Wang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Transplantation ◽  
Mouse Model ◽  
Tumor Progression ◽  
Nude Mouse ◽  
Orthotopic Liver Transplantation ◽  
Human Hepatocellular Carcinoma ◽  
Nude Mouse Model

Validation of ZMYND8 as a New Treatment Target in Hepatocellular Carcinoma

2021 ◽  
Author(s):  
Sangjoon Choi ◽  
Keun-Woo Lee ◽  
Hyun Hee Koh ◽  
Sujin Park ◽  
So-Young Yeo ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Mouse Model ◽  
Nude Mouse ◽  
Early Recurrence ◽  
Soft Agar ◽  
Migration And Invasion ◽  
Nude Mouse Model ◽  
Chain Reactions ◽  
Hcc Cells

Abstract Background: ZMYND8 (zinc finger MYND (Myeloid, Nervy and DEAF-1)-type containing 8) has been known to play an important role in tumor regulation in various types of cancer. However, the results of ZMYND8 expression and their clinical significance in hepatocellular carcinoma (HCC) have not yet been published. In the present study, we investigate the expression of ZMYND8 protein and mRNA in HCC and elucidate its prognostic significance.Methods: ZMYND8 protein and mRNA expression in 283 and 234 HCCs were investigated using immunohistochemistry and quantitative real-time polymerase chain reactions. The relationships between ZMYND8 expression with clinicopathologic features and prognosis of HCC patients were evaluated. Furthermore, we performed the invasion, migration, apoptosis, soft agar formation assay and sphere formation assay in HCC cell lines, and evaluated tumorigenicity in a nude mouse model, after ZMYND8 knockdown. Results: Overexpression of ZMYND8 protein and mRNA was observed in 20.5% and 26.9% of HCC cases, respectively. High ZMYND8 expression showed significant correlations with microvascular invasion, high Edmondson grade, advanced American Joint Committee on Cancer (AJCC), and increased alpha-fetoprotein level. ZMYND8 mRNA overexpression was an independent prognostic factor for predicting early recurrence as well as short recurrence-free survival (RFS). Downregulation of ZMYND8 reduced migration and invasion of HCC cells, and promoted apoptosis of HCC cells in an in vitro model. In a xenograft nude mouse model, knockdown of ZMYND8 significantly reduced tumor growth.Conclusions: ZMYND8 mRNA overexpression could be a prognostic marker of shorter RFS in HCC patients after curative resection. ZMYND8 might play an important role in the proliferation and progression of HCC and could be a promising candidate for targeted therapy.

scholarly journals The Role of Bridge Therapy Prior to Orthotopic Liver Transplantation

2014 ◽  
Vol 12 (8) ◽  
pp. 1183-1191 ◽  
Author(s):  
Meena A. Prasad ◽  
Laura M. Kulik
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Transplantation ◽  
Tumor Progression ◽  
Orthotopic Liver Transplantation ◽  
Consensus Statement ◽  
Waiting List ◽  
Locoregional Therapy ◽  
Unresectable Hepatocellular Carcinoma ◽  
Bridge Therapy

Orthotopic liver transplantation (OLT) offers the best chance for cure in the setting of unresectable hepatocellular carcinoma (HCC). A consensus statement recommends locoregional therapy (LRT) be considered in patients with HCC who are expected to wait more than 6 months for OLT to diminish dropout from the waiting list because of tumor progression. This article reviews LRT as a bridge to OLT in patients with HCC.

Tumor-Educated Macrophages Stimulate Tumor Progression in an Orthotopic Human Pancreatic Cancer Nude Mouse Model

2012 ◽  
Vol 172 (2) ◽  
pp. 234
Author(s):  
R.S. Menen ◽  
M.K. Hassanein ◽  
M. Momiyama ◽  
A. Suetsugu ◽  
A.R. Moossa ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Mouse Model ◽  
Tumor Progression ◽  
Nude Mouse ◽  
Human Pancreatic Cancer ◽  
Nude Mouse Model
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