scholarly journals Inhibition of Proliferation of SGC7901 and BGC823 Human Gastric Cancer Cells by Ursolic Acid Occurs Through a Caspase-Dependent Apoptotic Pathway

2019 ◽  
Vol 25 ◽  
pp. 6846-6854 ◽  
Author(s):  
Jing Zhang ◽  
Fengjun Liu ◽  
Xin Zhang
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Ursolic Acid ◽  
Human Gastric Cancer ◽  
Gastric Cancer Cells ◽  
Apoptotic Pathway ◽  
Inhibition Of Proliferation

scholarly journals METTL3 promotes the proliferation and mobility of gastric cancer cells

Open Medicine ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Sen Lin ◽  
Jianing Liu ◽  
Wen Jiang ◽  
Peng Wang ◽  
Chao Sun ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Migration And Invasion ◽  
Human Gastric Cancer ◽  
Akt Signaling Pathway ◽  
Gastric Cancer Cells ◽  
Apoptotic Pathway ◽  
Mechanistic Investigation

AbstractMethyltransferase-like 3 (METTL3) was originally known to be responsible for N6-methyladenosine (m6A) modification of mRNA. Recent studies have found that METTL3 plays important roles in a variety of tumors by regulating the translation of oncogenes. However, the functional and regulating mechanisms of METTL3 in human gastric cancer have not yet been understood. Here we knocked down METTL3 in human gastric cancer cell lines, AGS and MKN45, by using shRNA transfection. RT-qPCR assay and western blotting verified the effectiveness of RNA interference on mRNA and protein levels, respectively. Then we found that METTL3 knockdown inhibited cell proliferation, migration and invasion in AGS and MKN45 cells. Moreover, METTL3 knockdown decreased Bcl2 and increased Bax and active Caspase-3 in gastric cancer cells, which suggested the apoptotic pathway was activated. Mechanistic investigation suggested that METTL3 led to inactivation of the AKT signaling pathway in human gastric cancer cells, including decreased phosphorylation levels of AKT and expression of down-stream effectors p70S6K and Cyclin D1. In conclusion, our study reveals that down-regulation of METTL3 inhibits the proliferation and mobility of human gastric cancer cells and leads to inactivation of the AKT signaling pathway, suggesting that METTL3 may be a potential target for the treatment of human gastric cancer.

scholarly journals Crocodile choline from Crocodylus siamensis induces apoptosis of human gastric cancer

Tumor Biology ◽  
2017 ◽  
Vol 39 (3) ◽  
pp. 101042831769432 ◽  
Author(s):  
Xiao-Mei Mao ◽  
Qi-Rui Fu ◽  
Hua-Liang Li ◽  
Ya-Hui Zheng ◽  
Shu-Ming Chen ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Notch Pathway ◽  
Human Gastric Cancer ◽  
Short Interfering Rna ◽  
Gastric Cancer Cells ◽  
Apoptotic Pathway ◽  
Mitochondrial Apoptotic Pathway ◽  
Crocodylus Siamensis ◽  
Interfering Rna

Crocodile choline, an active compound isolated from Crocodylus siamensis, was found to exert potent anti-cancer activities against human gastric cancer cells in vitro and in vivo. Our study revealed that crocodile choline led to cell cycle arrest at the G2/M phase through attenuating the expressions of cyclins, Cyclin B1, and CDK-1. Furthermore, crocodile choline accelerated apoptosis through the mitochondrial apoptotic pathway with the decrease in mitochondrial membrane potential, the increase in reactive oxygen species production and Bax/Bcl-2 ratio, and the activation of caspase-3 along with the release of cytochrome c. In addition, this study, for the first time, shows that Notch pathway is remarkably deregulated by crocodile choline. The combination of crocodile choline and Notch1 short interfering RNA led to dramatically increased cytotoxicity than observed with either agent alone. Notch1 short interfering RNA sensitized and potentiated the capability of crocodile choline to suppress the cell progression and invasion of gastric cancer. Taken together, these data suggested that crocodile choline was a potent progression inhibitor of gastric cancer cells, which was correlated with mitochondrial apoptotic pathway and Notch pathway. Combining Notch1 inhibitors with crocodile choline might represent a novel approach for gastric cancer.

Helicobacter pyloriinduces Snail expression through ROS-mediated activation of Erk and inactivation of GSK-3β in human gastric cancer cells

2016 ◽  
Vol 55 (12) ◽  
pp. 2236-2246 ◽  
Author(s):  
Hoang-Kieu-Chi Ngo ◽  
Hee Geum Lee ◽  
Juan-Yu Piao ◽  
Xiancai Zhong ◽  
Ha-Na Lee ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Human Gastric Cancer ◽  
Gastric Cancer Cells ◽  
Snail Expression ◽  
Gsk 3Β

scholarly journals TET2 regulates LncRNA-ANRIL expression and inhibits the growth of human gastric cancer cells

IUBMB Life ◽  
2016 ◽  
Vol 68 (5) ◽  
pp. 355-364 ◽  
Author(s):  
Wei Deng ◽  
Jin Wang ◽  
Jun Zhang ◽  
Jun Cai ◽  
Zhigang Bai ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Human Gastric Cancer ◽  
Gastric Cancer Cells
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