scholarly journals Comparison of the Analgesic and Anti-Inflammatory Effects of Xiaoyuningkun Decoction with Cynanchum Paniculatum and Fukeqianjin in a Mouse Model of Pelvic Inflammatory Disease

2019 ◽  
Vol 25 ◽  
pp. 9094-9102 ◽  
Author(s):  
Bixin Tang ◽  
Kunlun Wu ◽  
Qingyi Meng ◽  
Fang Wang
Keyword(s):  
Mouse Model ◽  
Pelvic Inflammatory Disease ◽  
Inflammatory Disease ◽  
Cynanchum Paniculatum ◽  
Anti Inflammatory

scholarly journals Anti-Inflammatory Effect of Feiyangchangweiyan Capsule on Rat Pelvic Inflammatory Disease through JNK/NF-κB Pathway

2018 ◽  
Vol 2018 ◽  
pp. 1-10
Author(s):  
Yao Li ◽  
Yang Liu ◽  
Qian Yang ◽  
Zhihui Shi ◽  
Yanhua Xie ◽  
...  
Keyword(s):  
Pelvic Inflammatory Disease ◽  
Inflammatory Disease ◽  
Genital Tract ◽  
Nuclear Translocation ◽  
Immunohistochemical Analysis ◽  
Dependent Manner ◽  
Preventive Effect ◽  
Anti Inflammatory ◽  
Dose Dependent ◽  
Inflammatory Effect

Objectives. In this study, we aimed to illustrate the preventive effect and possible mechanisms of Feiyangchangweiyan capsule (FYCWYC) on rat pelvic inflammatory disease (PID) model. Methods. To construct the rat PID model, upper genital tract was infected by multipathogen, and then drugs were orally administered for 8 days. The histological examination, immunohistochemical analysis, and ELISA were carried out. Furthermore, Western blotting was used to analyze the expression of Akt, MAPKs, NF-κB p65, and IκB-α in uterus. Results. As the results showed, infiltrations of neutrophils and lymphocytes in uterus were significantly suppressed, and IL-1β, IL-6, CXCL-1, and TNF-α were also reduced in a dose-dependent manner. We also found that FYCWYC inhibited apoptosis induced by infection. Furthermore, FYCWYC could block the infection-induced nuclear translocation of NF-κB. We found that FYCWYC treatment only decreased the phosphorylation of JNK induced by infection and had no effects on Akt and P38. Additional, the effects of SP600125, an inhibitor of phospho-JNK, were similar to the results of FYCWYC. Conclusions. Taken together, our results demonstrated that FYCWYC had anti-inflammatory effect in pathogen-induced PID model, and the mechanism might be through inhibiting NF-κB nuclear translocation which is mediated by JNK.

scholarly journals The Anti-Inflammatory Effect of Feiyangchangweiyan Capsule and Its Main Components on Pelvic Inflammatory Disease in Rats via the Regulation of the NF-κB and BAX/BCL-2 Pathway

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Yao Li ◽  
Qian Yang ◽  
Zhi-hui Shi ◽  
Min Zhou ◽  
Li Yan ◽  
...  
Keyword(s):  
Pelvic Inflammatory Disease ◽  
Inflammatory Disease ◽  
Pathogenic Bacteria ◽  
Inflammatory Cells ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Pathogen ◽  
Anti Inflammatory ◽  
Main Components ◽  
Female Specific ◽  
Inflammatory Effect

Although gastroenteritis and pelvic inflammatory disease (PID) occur in the gastrointestinal tract and pelvis, respectively, they display similar pathogeneses. The incidence of inflammation in these conditions is usually associated with dysbacteriosis, and, at times, they are caused by the same pathogenic bacteria, Escherichia coli and Streptococcus aureus. Feiyangchangweiyan capsule (FYC) is a traditional Chinese patent medicine that is widely used to treat bacterial dysentery and acute and chronic gastroenteritis. However, whether it has an effect on PID is unclear. The aim of this study was to investigate the anti-inflammatory effect of FYC and its main components, gallic acid (GA), ellagic acid (EA), and syringin (SY), on a pathogen-induced PID model and illustrate their potential mechanism of action. Female specific pathogen-free SD rats (n = 1110) were randomly divided into control, PID, FYC, GA, EA, SY, GA + EA, GA + SY, EA + SY, GA + EA + SY, and Fuke Qianjin capsule (FKC) positive groups. Histological examination and enzyme-linked immunosorbent assay (ELISA) were carried out as well as western blot analysis to detect the expression of NF-κB, BAX, BCL-2, and JNK. In this study, FYC and its main components dramatically suppressed the infiltration of inflammatory cells, reduced the production of IL-1β, TNF-α, and MCP-1, and elevated the IL-10 level to varying degrees. We also found that FYC and its main components inhibited the expression of BAX induced by infection and increased the expression of Bcl-2. FYC, GA, EA, and SY could also block the activation of the NF-κB pathway. Finally, we found that the phosphorylation of JNK could be decreased by FYC, GA, and SY. FYC and its main components exhibit anti-inflammatory effect on a pathogen-induced PID model by regulating the NF-κB and apoptosis signaling pathways.

scholarly journals Early MicroRNA Expression Profile as a Prognostic Biomarker for the Development of Pelvic Inflammatory Disease in a Mouse Model of Chlamydial Genital Infection

mBio ◽  
2014 ◽  
Vol 5 (3) ◽  
Author(s):  
Laxmi Yeruva ◽  
Garry S. A. Myers ◽  
Nicole Spencer ◽  
Heather Huot Creasy ◽  
Nancy E. Adams ◽  
...  
Keyword(s):  
Mouse Model ◽  
Pelvic Inflammatory Disease ◽  
Inflammatory Disease ◽  
Expression Profiles ◽  
Tissue Response ◽  
Pcr Analysis ◽  
Genital Infection ◽  
Content Type ◽  
Cytokine Responses ◽  
Increased Risk

ABSTRACTIt is not currently possible to predict the probability of whether a woman with a chlamydial genital infection will develop pelvic inflammatory disease (PID). To determine if specific biomarkers may be associated with distinct chlamydial pathotypes, we utilized twoChlamydia muridarumvariants (C. muridarumVar001 [CmVar001] and CmVar004) that differ in their abilities to elicit upper genital tract pathology in a mouse model. CmVar004 has a lower growth ratein vitroand induces pathology in only 20% of C57BL/6 mouse oviducts versus 83.3% of oviducts in CmVar001-infected mice. To determine if chemokine and cytokine production within 24 h of infection is associated with the outcome of pathology, levels of 15 chemokines and cytokines were measured. CmVar004 infection induced significantly lower levels of CXCL1, CXCL2, tumor necrosis factor alpha (TNF-α), and CCL2 in comparison to CmVar001 infection with similar rRNA (rs16) levels forChlamydiae. A combination of microRNA (miRNA) sequencing and quantitative real-time PCR (qRT-PCR) analysis of 134 inflammation-related miRNAs was performed 24 h postinfection to determine if the chemokine/cytokine responses would also be reflected in miRNA expression profiles. Interestingly, 12 miRNAs (miR-135a-5p, miR298-5p, miR142-3p, miR223-3p, miR299a-3p, miR147-3p, miR105, miR325-3p, miR132-3p, miR142-5p, miR155-5p, and miR-410-3p) were overexpressed during CmVar004 infection compared to CmVar001 infection, inversely correlating with the respective chemokine/cytokine responses. To our knowledge, this is the first report demonstrating that early biomarkers elicited in the host can differentiate between two pathological variants of chlamydiae and be predictive of upper tract disease.IMPORTANCEIt is apparent that an infecting chlamydial population consists of multiple genetic variants with differing capabilities of eliciting a pathological response; thus, it may be possible to identify biomarkers specific for a given virulence pathotype. miRNAs are known to regulate genes that in turn regulate signaling pathways involved in disease pathogenesis. Importantly, miRNAs are stable and can reflect a tissue response and therefore have the potential to be biomarkers of disease severity. Currently, with respect to chlamydial infections, there is no way to predict whether an infected patient is more or less likely to develop PID. However, data presented in this study indicate that the expression of a specific miRNA profile associated with a virulent variant early in the infection course may be predictive of an increased risk of pelvic inflammatory disease, allowing more aggressive treatment before significant pathology develops.

scholarly journals Assessment of quality of life and psychological condition of women with chronic inflammatory processes of the pelvic organs on the background of pelvic pain

2021 ◽  
Vol 2 ◽  
pp. 69-72
Author(s):  
N. Matviikiv
Keyword(s):  
Quality Of Life ◽  
Pelvic Inflammatory Disease ◽  
Pelvic Pain ◽  
Inflammatory Disease ◽  
Chronic Pelvic Pain ◽  
Pelvic Organs ◽  
Inflammatory Processes ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

The objective: was to assess the psychological status and quality of life of women in the treatment of recurrent pelvic inflammatory disease with chronic pelvic pain syndrome.Materials and methods. We observed 100 women of reproductive age who suffered from chronic pelvic pain syndrome in chronic inflammatory processes of the pelvic organs. All women were prescribed antibacterial therapy in combination with antihypoxants and antiplatelet agents. The first group included women (n=48) who were prescribed analgesics domestically due to existing contraindications to the use of non-steroidal anti-inflammatory drugs. The second group of women (n=52) received a rectally non-steroidal anti-inflammatory drug. The second group of women (n=52) received a rectally nonsteroidal anti-inflammatory agent. Patients were interviewed 3 months after treatment: using the Visual Analog Scale (VAS), the McGill Questionnaire, the Spielberger–Hanin Questionnaire, the Depression Center of the US Center for Epidemiological Research, the Beck Depression Rating Scale, and the SF-36 Questionnaire.Results. According to the results of the assessment of quality of life and changes in psychological state, we noticed the following differences. In the group of women who received analgesic medium before muscle therapy, the change in muscle changed but slightly compared with the group of women who were offered the use of nonsteroidal anti-inflammatory drugs. Indicators of quality of life and psychological condition in women of the second group have significantly improved.Conclusions. The results of this study indicate the relationship and comprehensive approach in the treatment of pelvic inflammatory disease, which is accompanied by pelvic pain.

Integrated method of treatment the chronic pelvic pain in inflammtory processes of the women pelvic organs

2016 ◽  
pp. 104-106
Author(s):  
L.P. Grek ◽  
Keyword(s):  
Pelvic Inflammatory Disease ◽  
Inflammatory Cytokines ◽  
Pelvic Pain ◽  
Inflammatory Disease ◽  
Chronic Pelvic Pain ◽  
Pelvic Pain Syndrome ◽  
Therapeutic Treatment ◽  
Pelvic Organs ◽  
Anti Inflammatory

The article presents the role of pro- and anti-inflammatory cytokines in the process of chronic pelvic pain (CPP) in women with pelvic inflammatory disease; therapeutic treatment for the prevention of relapse and psychological disorders. The objective: to determinate the role of the pro - and anti-inflammatory cytokines into the implementation of chronic pelvic pain syndrome (CPP) and to measure the performance of treatment which including pathophysiological factors of the woman’s pain with pelvic inflammatory disease Patients and methods: The study involved 75 women with PID. The main group consisted of 40 women with CPPS, Group 2 – 35 patients with painless passage of (the comparison group). The assessment of pain using a visual analogue scale (VAS); Pain Questionnaire McGill; conducted psychological testing, determined by the content of cytokines (IL-10, IL-6, TNF-a) in the blood serum. Results. Significantly elevated levels of TNF-a in the celebrated in the first group compared with the second (p<0.001), which is directly correlated with the duration of CPPS (r=0.422; p<0.001), the intensity of pelvic pain VAS (r=0.469; p<0.001), as well as moderate and severe symptoms of depression degree (r=0.333; p<0.05). Conclusions. The therapeutic treatment what we offered contributed to the regression of the pain after 1 month as determined by VAS 45.3±1.4 mm and 3 months 31.3±1.4 mm; reduction of reactive anxiety and depressive disorders detected in 84.7% of patients; improving psycho-emotional and general condition, normalization of sleep observed in 88.3% of women. Key words: inflammatory diseases of the pelvic organs, chronic pelvic pain, cytokines, personal anxiety.

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