scholarly journals Concentrated Growth Factors Can Inhibit Photoaging Damage Induced by Ultraviolet A (UVA) on the Human Dermal Fibroblasts In Vitro

2019 ◽  
Vol 25 ◽  
pp. 3739-3749 ◽  
Author(s):  
Junyin Chen ◽  
Dandan Jiao ◽  
Meng Zhang ◽  
Shihong Zhong ◽  
Tai Zhang ◽  
...  
Keyword(s):  
Growth Factors ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A ◽  
Concentrated Growth Factors

Singlet oxygen is an early intermediate in cytokine-dependent ultraviolet-A induction of interstitial collagenase in human dermal fibroblasts in vitro

FEBS Letters ◽  
1997 ◽  
Vol 413 (2) ◽  
pp. 239-242 ◽  
Author(s):  
Meinhard Wlaschek ◽  
Jutta Wenk ◽  
Peter Brenneisen ◽  
Karlis Briviba ◽  
Agatha Schwarz ◽  
...  
Keyword(s):  
Singlet Oxygen ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A ◽  
Interstitial Collagenase

In vitro adhesion of human dermal fibroblasts on iron cross-linked alginate films

2009 ◽  
Vol 4 (2) ◽  
pp. 025008 ◽  
Author(s):  
Ikuko Machida-Sano ◽  
Yasushi Matsuda ◽  
Hideo Namiki
Keyword(s):  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
In Vitro Adhesion

scholarly journals Protective Effect of Sulfated Polysaccharides from Celluclast-Assisted Extract of Hizikia fusiforme Against Ultraviolet B-Induced Skin Damage by Regulating NF-κB, AP-1, and MAPKs Signaling Pathways In Vitro in Human Dermal Fibroblasts

Marine Drugs ◽  
2018 ◽  
Vol 16 (7) ◽  
pp. 239 ◽  
Author(s):  
Lei Wang ◽  
WonWoo Lee ◽  
Jae Oh ◽  
Yong Cui ◽  
BoMi Ryu ◽  
...  
Keyword(s):  
Protective Effect ◽  
Signaling Pathways ◽  
Dermal Fibroblasts ◽  
Sulfated Polysaccharides ◽  
Dependent Manner ◽  
Human Dermal Fibroblasts ◽  
Skin Damage ◽  
Hizikia Fusiforme ◽  
Hdf Cells

Our previous study evaluated the antioxidant activities of sulfated polysaccharides from Celluclast-assisted extract of Hizikia fusiforme (HFPS) in vitro in Vero cells and in vivo in zebrafish. The results showed that HFPS possesses strong antioxidant activity and suggested the potential photo-protective activities of HFPS. Hence, in the present study, we investigated the protective effects of HFPS against ultraviolet (UV) B-induced skin damage in vitro in human dermal fibroblasts (HDF cells). The results indicate that HFPS significantly reduced intracellular reactive oxygen species (ROS) level and improved the viability of UVB-irradiated HDF cells in a dose-dependent manner. Furthermore, HFPS significantly inhibited intracellular collagenase and elastase activities, remarkably protected collagen synthesis, and reduced matrix metalloproteinases (MMPs) expression by regulating nuclear factor kappa B (NF-κB), activator protein 1 (AP-1), and mitogen-activated protein kinases (MAPKs) signaling pathways in UVB-irradiated HDF cells. These results suggest that HFPS possesses strong UV protective effect, and can be a potential ingredient in the pharmaceutical and cosmetic industries.

Photobiomodulation with 590 nm Wavelength Delays the Telomere Shortening and Replicative Senescence of Human Dermal Fibroblasts In Vitro

2020 ◽  
Vol 38 (11) ◽  
pp. 656-660
Author(s):  
Borislav Arabadjiev ◽  
Roumen Pankov ◽  
Ivelina Vassileva ◽  
Lyuben Sashov Petrov ◽  
Ivan Buchvarov
Keyword(s):  
Replicative Senescence ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Telomere Shortening

scholarly journals Senescence of Human Fibroblasts after Psoralen Photoactivation Is Mediated by ATR Kinase and Persistent DNA Damage Foci at Telomeres

2006 ◽  
Vol 17 (4) ◽  
pp. 1758-1767 ◽  
Author(s):  
Miriam Grosse Hovest ◽  
Nicole Brüggenolte ◽  
Kijawasch Shah Hosseini ◽  
Thomas Krieg ◽  
Gernot Herrmann
Keyword(s):  
Dna Damage ◽  
Human Fibroblasts ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Ultraviolet A ◽  
Cross Link ◽  
Telomeric Dna ◽  
Histone H2ax ◽  
Senescent Phenotype ◽  
Atr Kinase

Cellular senescence is a phenotype that is likely linked with aging. Recent concepts view different forms of senescence as permanently maintained DNA damage responses partially characterized by the presence of senescence-associated DNA damage foci at dysfunctional telomeres. Irradiation of primary human dermal fibroblasts with the photosensitizer 8-methoxypsoralen and ultraviolet A radiation (PUVA) induces senescence. In the present study, we demonstrate that senescence after PUVA depends on DNA interstrand cross-link (ICL) formation that activates ATR kinase. ATR is necessary for the manifestation and maintenance of the senescent phenotype, because depletion of ATR expression before PUVA prevents induction of senescence, and reduction of ATR expression in PUVA-senesced fibroblasts releases cells from growth arrest. We find an ATR-dependent phosphorylation of the histone H2AX (γ-H2AX). After PUVA, ATR and γ-H2AX colocalize in multiple nuclear foci. After several days, only few predominantly telomere-localized foci persist and telomeric DNA can be coimmunoprecipitated with ATR from PUVA-senesced fibroblasts. We thus identify ATR as a novel mediator of telomere-dependent senescence in response to ICL induced by photoactivated psoralens.

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