scholarly journals Overexpression of DNA (Cytosine-5)-Methyltransferase 1 (DNMT1) And DNA (Cytosine-5)-Methyltransferase 3A (DNMT3A) Is Associated with Aggressive Behavior and Hypermethylation of Tumor Suppressor Genes in Human Pituitary Adenomas

2018 ◽  
Vol 24 ◽  
pp. 4841-4850 ◽  
Author(s):  
Hou-Shi Ma ◽  
Elaine Lu Wang ◽  
Wen-Fei Xu ◽  
Shozo Yamada ◽  
Katsuhiko Yoshimoto ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Aggressive Behavior ◽  
Pituitary Adenomas ◽  
Tumor Suppressor Genes ◽  
Human Pituitary ◽  
Suppressor Genes ◽  
Human Pituitary Adenomas ◽  
Methyltransferase 1

scholarly journals Inactivation of RASSF1A tumor suppressor gene by aberrant promoter hypermethylation in human pituitary adenomas

2005 ◽  
Vol 85 (4) ◽  
pp. 464-473 ◽  
Author(s):  
Zhi Rong Qian ◽  
Toshiaki Sano ◽  
Katsuhiko Yoshimoto ◽  
Shozo Yamada ◽  
Akira Ishizuka ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Tumor Suppressor Gene ◽  
Pituitary Adenomas ◽  
Promoter Hypermethylation ◽  
Suppressor Gene ◽  
Human Pituitary ◽  
Human Pituitary Adenomas

scholarly journals RX-3117 (Fluorocyclopentenyl-Cytosine)-Mediated Down-Regulation of DNA Methyltransferase 1 Leads to Protein Expression of Tumor-Suppressor Genes and Increased Functionality of the Proton-Coupled Folate Carrier

2020 ◽  
Vol 21 (8) ◽  
pp. 2717 ◽  
Author(s):  
Dzjemma Sarkisjan ◽  
Joris R. Julsing ◽  
Btissame El Hassouni ◽  
Richard J. Honeywell ◽  
Ietje Kathmann ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Protein Expression ◽  
Tumor Suppressor Genes ◽  
Dna Methyltransferase ◽  
Functional Assay ◽  
Dna Methyltransferase 1 ◽  
Suppressor Genes ◽  
Resistant Variant ◽  
E Cadherin ◽  
Methyltransferase 1

(1) Background: RX-3117 (fluorocyclopentenyl-cytosine) is a cytidine analog that inhibits DNA methyltransferase 1 (DNMT1). We investigated the mechanism and potential of RX-3117 as a demethylating agent in several in vitro models. (2) Methods: we used western blotting to measure expression of several proteins known to be down-regulated by DNA methylation: O6-methylguanine-DNA methyltransferase (MGMT) and the tumor-suppressor genes, p16 and E-cadherin. Transport of methotrexate (MTX) mediated by the proton-coupled folate transporter (PCFT) was used as a functional assay. (3) Results: RX-3117 treatment decreased total DNA-cytosine-methylation in A549 non-small cell lung cancer (NSCLC) cells, and induced protein expression of MGMT, p16 and E-cadherin in A549 and SW1573 NSCLC cells. Leukemic CCRF-CEM cells and the MTX-resistant variant (CEM/MTX, with a deficient reduced folate carrier) have a very low expression of PCFT due to promoter hypermethylation. In CEM/MTX cells, pre-treatment with RX-3117 increased PCFT-mediated MTX uptake 8-fold, and in CEM cells 4-fold. With the reference hypomethylating agent 5-aza-2′-deoxycytidine similar values were obtained. RX-3117 also increased PCFT gene expression and PCFT protein. (4) Conclusion: RX-3117 down-regulates DNMT1, leading to hypomethylation of DNA. From the increased protein expression of tumor-suppressor genes and functional activation of PCFT, we concluded that RX-3117 might have induced hypomethylation of the promotor.

scholarly journals MUC1-C induces DNA methyltransferase 1 and represses tumor suppressor genes in acute myeloid leukemia

Oncotarget ◽  
2016 ◽  
Vol 7 (26) ◽  
pp. 38974-38987 ◽  
Author(s):  
Ashujit Tagde ◽  
Hasan Rajabi ◽  
Dina Stroopinsky ◽  
Reddy Gali ◽  
Maroof Alam ◽  
...  
Keyword(s):  
Acute Myeloid Leukemia ◽  
Tumor Suppressor ◽  
Tumor Suppressor Genes ◽  
Myeloid Leukemia ◽  
Dna Methyltransferase ◽  
Dna Methyltransferase 1 ◽  
Suppressor Genes ◽  
Acute Myeloid ◽  
Methyltransferase 1

Abstract #826 Sox 2 Expression in Human Pituitary Adenomas

2018 ◽  
Vol 24 ◽  
pp. 186
Author(s):  
Cristina Capatina ◽  
Anca Campeanu ◽  
Marius Raica ◽  
Mihail Coculescu ◽  
Catalina Poiana
Keyword(s):  
Pituitary Adenomas ◽  
Human Pituitary ◽  
Human Pituitary Adenomas
Sign in / Sign up

Export Citation Format

Share Document