scholarly journals Curcumin Treatment is Associated with Increased Expression of the N-Methyl-D-Aspartate Receptor (NMDAR) Subunit, NR2A, in a Rat PC12 Cell Line Model of Alzheimer’s Disease Treated with the Acetyl Amyloid-β Peptide, Aβ(25–35)

2018 ◽  
Vol 24 ◽  
pp. 2693-2699 ◽  
Author(s):  
Wei Qian ◽  
Haiyan Li ◽  
Ningfeng Pan ◽  
Changchun Zhang
Keyword(s):  
Cell Line ◽  
Pc12 Cell ◽  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Curcumin Treatment ◽  
Pc12 Cell Line ◽  
Aspartate Receptor ◽  
Cell Line Model ◽  
Model Of Alzheimer’S Disease ◽  
Nmdar Subunit

scholarly journals Establishment and Characterization of Methylmercury-Resistant PC12 Cell Line

1994 ◽  
Vol 102 ◽  
pp. 313 ◽  
Author(s):  
Kyoko Miura ◽  
Thomas W. Clarkson ◽  
Kazumasa Ikeda ◽  
Akira Naganuma ◽  
Nobumasa Imura
Keyword(s):  
Cell Line ◽  
Pc12 Cell ◽  
Pc12 Cell Line

scholarly journals Synaptotagmin VII Is Targeted to Dense-core Vesicles and Regulates Their Ca2+-dependent Exocytosis in PC12 Cells

2004 ◽  
Vol 279 (50) ◽  
pp. 52677-52684 ◽  
Author(s):  
Mitsunori Fukuda ◽  
Eiko Kanno ◽  
Megumi Satoh ◽  
Chika Saegusa ◽  
Akitsugu Yamamoto
Keyword(s):  
Plasma Membrane ◽  
Neuropeptide Y ◽  
Cell Line ◽  
Pc12 Cells ◽  
Pc12 Cell ◽  
Small Interfering Rna ◽  
Dense Core ◽  
Dense Core Vesicles ◽  
Pc12 Cell Line ◽  
Interfering Rna

It has recently been proposed that synaptotagmin (Syt) VII functions as a plasma membrane Ca2+sensor for dense-core vesicle exocytosis in PC12 cells based on the results of transient overexpression studies using green fluorescent protein (GFP)-tagged Syt VII; however, the precise subcellular localization of Syt VII is still a matter of controversy (plasma membraneversussecretory granules). In this study we established a PC12 cell line “stably expressing” the Syt VII-GFP molecule and demonstrated by immunocytochemical and immunoelectron microscopic analyses that the Syt VII-GFP protein is localized on dense-core vesicles as well as in other intracellular membranous structures, such as thetrans-Golgi network and lysosomes. Syt VII-GFP forms a complex with endogenous Syts I and IX, but not with Syt IV, and it colocalize well with Syts I and IX in the cellular processes (where dense-core vesicles are accumulated) in the PC12 cell line. We further demonstrated by an N-terminal antibody-uptake experiment that Syt VII-GFP-containing dense-core vesicles undergo Ca2+-dependent exocytosis, the same as endogenous Syt IX-containing vesicles. Moreover, silencing of Syt VII-GFP with specific small interfering RNA dramatically reduced high KCl-dependent neuropeptide Y secretion from the stable PC12 cell line (∼60% of the control cells), whereas the same small interfering RNA had little effect on neuropeptide Y secretion from the wild-type PC12 cells (∼85–90% of the control cells), indicating that the level of endogenous expression of Syt VII molecules must be low. Our results indicate that the targeting of Syt VII-GFP molecules to specific membrane compartment(s) is affected by the transfection method (transient expressionversusstable expression) and suggested that Syt VII molecule on dense-core vesicles functions as a vesicular Ca2+sensor for exocytosis in endocrine cells.

Amyloid-β disrupts unitary calcium entry through endothelial NMDA receptors in mouse cerebral arteries

2021 ◽  
pp. 0271678X2110395
Author(s):  
Emily C Peters ◽  
Michael T Gee ◽  
Lukas N Pawlowski ◽  
Allison M Kath ◽  
Felipe D Polk ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cerebral Arteries ◽  
Amyloid Β ◽  
Amyloid Angiopathy ◽  
Wild Type ◽  
Pial Arteries ◽  
Aspartate Receptor ◽  
Model Of Alzheimer’S Disease ◽  
Intracellular Ca ◽  
Cerebral Amyloid

Transient increases in intracellular Ca2+ activate endothelium-dependent vasodilatory pathways. This process is impaired in cerebral amyloid angiopathy, where amyloid- β(1-40) accumulates around blood vessels. In neurons, amyloid- β impairs the Ca2+-permeable N-methyl-D-aspartate receptor (NMDAR), a mediator of endothelium-dependent dilation in arteries. We hypothesized that amyloid- β(1-40) reduces NMDAR-elicited Ca2+ signals in mouse cerebral artery endothelial cells, blunting dilation. Cerebral arteries isolated from 4-5 months-old, male and female cdh5:Gcamp8 mice were used for imaging of unitary Ca2+ influx through NMDAR ( NMDAR sparklets) and intracellular Ca2+ transients. The NMDAR agonist NMDA (10 µmol/L) increased frequency of NMDAR sparklets and intracellular Ca2+ transients in endothelial cells; these effects were prevented by NMDAR antagonists D-AP5 and MK-801. Next, we tested if amyloid- β(1-40) impairs NMDAR-elicited Ca2+ transients. Cerebral arteries incubated with amyloid- β(1-40) (5 µmol/L) exhibited reduced NMDAR sparklets and intracellular Ca2+ transients. Lastly, we observed that NMDA-induced dilation of pial arteries is reduced by acute intraluminal amyloid- β(1-40), as well as in a mouse model of Alzheimer’s disease, the 5x-FAD, linked to downregulation of Grin1 mRNA compared to wild-type littermates. These data suggest that endothelial NMDAR mediate dilation via Ca2+-dependent pathways, a process disrupted by amyloid- β(1-40) and impaired in 5x-FAD mice.

scholarly journals Establishment and characterization of methylmercury-resistant PC12 cell line.

1994 ◽  
Vol 102 (suppl 3) ◽  
pp. 313-315 ◽  
Author(s):  
K Miura ◽  
T W Clarkson ◽  
K Ikeda ◽  
A Naganuma ◽  
N Imura
Keyword(s):  
Cell Line ◽  
Pc12 Cell ◽  
Pc12 Cell Line

scholarly journals Effect of human skin explants on the neurite growth of the PC12 cell line

2013 ◽  
Vol 22 (3) ◽  
pp. 224-225 ◽  
Author(s):  
Nicolas Lebonvallet ◽  
Jean-Pierre Pennec ◽  
Christelle Le Gall ◽  
Ulysse Pereira ◽  
Nicholas Boulais ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Skin ◽  
Pc12 Cell ◽  
Neurite Growth ◽  
Pc12 Cell Line ◽  
Skin Explants

Regulatory networks upon neurogenesis induction in PC12 cell line by small molecules

2019 ◽  
Vol 234 (10) ◽  
pp. 18813-18824
Author(s):  
Nafiseh Falsafi ◽  
Tahereh Soleimani ◽  
Hossein Fallahi ◽  
Mehri Azadbakht
Keyword(s):  
Small Molecules ◽  
Cell Line ◽  
Pc12 Cell ◽  
Regulatory Networks ◽  
Pc12 Cell Line
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