scholarly journals Inhibitory Effects of Plumbagin on Retinal Pigment Epithelial Cell Epithelial-Mesenchymal Transition In Vitro and In Vivo

2018 ◽  
Vol 24 ◽  
pp. 1502-1510 ◽  
Author(s):  
Haiting Chen ◽  
Huifang Wang ◽  
Jianbin An ◽  
Qingli Shang ◽  
Jingxue Ma
Keyword(s):  
Epithelial Cell ◽  
Retinal Pigment Epithelial ◽  
Epithelial Mesenchymal Transition ◽  
Retinal Pigment Epithelial Cell ◽  
Retinal Pigment ◽  
Inhibitory Effects ◽  
Mesenchymal Transition ◽  
Pigment Epithelial

scholarly journals Protective Effects of Fucoidan on Epithelial-Mesenchymal Transition of Retinal Pigment Epithelial Cells and Progression of Proliferative Vitreoretinopathy

2018 ◽  
Vol 46 (4) ◽  
pp. 1704-1715 ◽  
Author(s):  
Yao Zhang ◽  
Dongwan Zhao ◽  
Shuai Yang ◽  
Haipei Yao ◽  
Min Li ◽  
...  
Keyword(s):  
Retinal Pigment Epithelial ◽  
Epithelial Mesenchymal Transition ◽  
Retinal Pigment ◽  
Protective Effects ◽  
Mesenchymal Transition ◽  
Rpe Cells ◽  
Pigment Epithelial ◽  
Experimental Pvr ◽  
Tgf Β1

Background/Aims: Proliferative vitreoretinopathy (PVR) is a severe blinding complication of rhegmatogenous retinal detachment. Epithelial-mesenchymal transition (EMT) of retinal pigment epithelial (RPE) cells is thought to play a pivotal role in the pathogenesis of PVR. Fucoidan, a marine extract, reportedly has many benefits effects in a variety of tissues and organs such as anti-inflammation, anti-oxidative stress, and anti-carcinogenesis. In this study, we investigated the potential role of fucoidan on EMT in RPE cells and its effect on the development of PVR. Methods: MTS, Transwell, and collagen gel contraction assays were employed to measure the viability, migration, and contraction of RPE cells, respectively. mRNA and protein expression were evaluated via real-time quantitative PCR and western blot analysis, respectively. In vivo, a pigmented rabbit model of PVR was established to examine the anti-PVR effect of fucoidan. Results: Fucoidan reversed the transforming growth factor (TGF)-β1-induced EMT of RPE cells, including the increased expression of α-smooth muscle actin (α-SMA) and fibronectin and down-regulation of E-cadherin in human primary RPE cells. Moreover, the upregulation of phosphorylated Smad2/3 induced by TGF-β1 was suppressed by fucoidan. Fucoidan also inhibited the migration and contraction of RPE cells induced by TGF-β1. In vivo, fucoidan inhibited the progression of experimental PVR in rabbit eyes. Histological findings showed that fucoidan suppressed the formation of α-SMA-positive epiretinal membranes. Conclusion: Our findings regarding the protective effects of fucoidan on the EMT of RPE cells and experimental PVR suggest the potential clinical application of fucoidan as an anti-PVR agent.

Pleiotropic role of atorvastatin in regulation of human retinal pigment epithelial cell behaviors in vitro

2011 ◽  
Vol 93 (6) ◽  
pp. 842-851 ◽  
Author(s):  
Wen-Chuan Wu ◽  
Yu-Hung Lai ◽  
Ming-Chu Hsieh ◽  
Yo-Chen Chang ◽  
Meng-Hsien Wu ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment Epithelial Cell ◽  
Retinal Pigment ◽  
Cell Behaviors ◽  
Pigment Epithelial

scholarly journals In Vivo Autofluorescence Imaging of the Human and Macaque Retinal Pigment Epithelial Cell Mosaic

2009 ◽  
Vol 50 (3) ◽  
pp. 1350 ◽  
Author(s):  
Jessica I. W. Morgan ◽  
Alfredo Dubra ◽  
Robert Wolfe ◽  
William H. Merigan ◽  
David R. Williams
Keyword(s):  
Epithelial Cell ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment Epithelial Cell ◽  
Retinal Pigment ◽  
Autofluorescence Imaging ◽  
Pigment Epithelial

scholarly journals A Semiautomated, Phenotypic, In Vitro Scratch Assay for Assessing Retinal Pigment Epithelial Cell Wound Healing

2020 ◽  
Vol 36 (4) ◽  
pp. 257-266
Author(s):  
Tina Storm ◽  
Iain Wilson ◽  
Ross Campbell ◽  
Arantxa Bolinches-Amorós ◽  
Angela J. Russell ◽  
...  
Keyword(s):  
Wound Healing ◽  
Epithelial Cell ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment Epithelial Cell ◽  
Retinal Pigment ◽  
Scratch Assay ◽  
Pigment Epithelial
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