scholarly journals High CXC Chemokine Ligand 16 (CXCL16) Expression Promotes Proliferation and Metastasis of Lung Cancer via Regulating the NF-κB Pathway

2018 ◽  
Vol 24 ◽  
pp. 405-411 ◽  
Author(s):  
Kun Liang ◽  
Yanru Liu ◽  
Dun Eer ◽  
Jingbin Liu ◽  
Fan Yang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cxc Chemokine ◽  
Chemokine Ligand ◽  
Proliferation And Metastasis ◽  
Cxcl16 Expression

scholarly journals P2.01-040 CXC Chemokine Receptor 3 and ELR Motif Negative CXC Chemokine Ligand Axis in Non-Small Cell Lung Cancer

2017 ◽  
Vol 12 (1) ◽  
pp. S809-S810
Author(s):  
Artjoms Spaks ◽  
Donats Breiva ◽  
Ilmars Tracums ◽  
Anastasija Bistrova ◽  
Krista Grigorovica ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Chemokine Receptor ◽  
Small Cell ◽  
Small Cell Lung ◽  
Cxc Chemokine ◽  
Chemokine Ligand

scholarly journals Human IP-9: A Keratinocyte-Derived High Affinity CXC-Chemokine Ligand for the IP-10/Mig Receptor (CXCR3)1

1999 ◽  
Vol 112 (5) ◽  
pp. 716-722 ◽  
Author(s):  
Cornelis P. Tensen ◽  
Jacoba Flier ◽  
Elizabeth M.H. van der Raaij-Helmer ◽  
Shakun Sampat-Sardjoepersad ◽  
Roel C. van der Schors ◽  
...  
Keyword(s):  
High Affinity ◽  
Cxc Chemokine ◽  
Chemokine Ligand

scholarly journals Secreted CXCL12 (SDF-1) forms dimers under physiological conditions

2012 ◽  
Vol 442 (2) ◽  
pp. 433-442 ◽  
Author(s):  
Paramita Ray ◽  
Sarah A. Lewin ◽  
Laura Anne Mihalko ◽  
Sasha-Cai Lesher-Perez ◽  
Shuichi Takayama ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Extracellular Space ◽  
Mammalian Cells ◽  
Cell Signalling ◽  
Xenograft Model ◽  
Physiological Conditions ◽  
Cxc Chemokine ◽  
Chemokine Ligand ◽  
Chemokine Cxcl12 ◽  
And Function

Chemokine CXCL12 (CXC chemokine ligand 12) signalling through CXCR (CXC chemokine receptor) 4 and CXCR7 has essential functions in development and underlies diseases including cancer, atherosclerosis and autoimmunity. Chemokines may form homodimers that regulate receptor binding and signalling, but previous studies with synthetic CXCL12 have produced conflicting evidence for homodimerization. We used bioluminescence imaging with GL (Gaussia luciferase) fusions to investigate dimerization of CXCL12 secreted from mammalian cells. Using column chromatography and GL complementation, we established that CXCL12 was secreted from mammalian cells as both monomers and dimers. Secreted CXCL12 also formed homodimers in the extracellular space. Monomeric CXCL12 preferentially activated CXCR4 signalling through Gαi and Akt, whereas dimeric CXCL12 more effectively promoted recruitment of β-arrestin 2 to CXCR4 and chemotaxis of CXCR4-expressing breast cancer cells. We also showed that CXCR7 preferentially sequestered monomeric CXCL12 from the extracellular space and had minimal effects on dimeric CXCL12 in cell-based assays and an orthotopic tumour xenograft model of human breast cancer. These studies establish that CXCL12 secreted from mammalian cells forms homodimers under physiological conditions. Since monomeric and dimeric CXCL12 have distinct effects on cell signalling and function, our results have important implications for ongoing efforts to target CXCL12 pathways for therapy.

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