scholarly journals Construction and Comprehensive Analysis for Dysregulated Long Non-Coding RNA (lncRNA)-Associated Competing Endogenous RNA (ceRNA) Network in Gastric Cancer

2018 ◽  
Vol 24 ◽  
pp. 37-49 ◽  
Author(s):  
Fengxi Li ◽  
Chuiguo Huang ◽  
Qian Li ◽  
Xianghua Wu
Keyword(s):  
Gastric Cancer ◽  
Comprehensive Analysis ◽  
Competing Endogenous Rna ◽  
Cerna Network ◽  
Non Coding Rna ◽  
Long Non Coding Rna

scholarly journals Identification of Long Non-Coding RNA-Associated Competing Endogenous RNA Network in the Differentiation of Chicken Preadipocytes

Genes ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 795 ◽  
Author(s):  
Chen ◽  
Zhang ◽  
Zhang ◽  
Huang ◽  
Zhang ◽  
...  
Keyword(s):  
Adipogenic Differentiation ◽  
Target Prediction ◽  
Competing Endogenous Rna ◽  
Chicken Fat ◽  
Cerna Network ◽  
Non Coding Rna ◽  
Competing Endogenous Rna Network ◽  
Gene Expression Correlation ◽  
Long Non Coding Rna

Emerging evidence indicates that long noncoding RNAs (lncRNAs) play important roles in the regulation of cell differentiation by acting as competing endogenous RNA (ceRNA). However, the regulatory mechanisms of lncRNA and the lncRNA-associated ceRNA network involved in adipogenic differentiation of chicken preadipocytes remain elusive. Here, we first constructed the chicken preadipocyte in vitro induction model. Then, we identified differentially expressed lncRNAs (DELs), miRNAs (DEMis), and mRNAs (DEMs) between differentiated and undifferentiated preadipocytes. Furthermore, we constructed the lncRNA associated ceRNA network by gene expression correlation analysis and target prediction of DELs, DEMis, and DEMs. Finally, we determined twelve candidate lncRNA-miRNA-mRNA interactions from the lncRNA associated ceRNA network. Eight out of the twelve interactions were validated by RT-qPCR, indicating their potential role in the regulation of chicken preadipocytes differentiation. Among the eight interactions, TCONS_00026544-gga-miR-128-1-5p-RASD1, TCONS_00055280-gga-miR-135a-5p-JAM3, TCONS_00055280-gga-miR-135a-5p-GPR133, TCONS_00055280-gga-miR-135a-5p-CLDN1, and TCONS_00055280-gga-miR-135a-5p-TMEM123 may promote adipogenic differentiation of chicken preadipocytes while TCONS_00057272-gga-miR-146a-3p-FOXO6, TCONS_00057242-gga-miR-6615-3p-FOXO6, and TCONS_00057242-gga-miR-6615-3p-ENSGALT00000043224 have the opposite effects. Our results not only provide novel insights into ceRNA roles of lncRNAs in chicken preadipocytes differentiation and but also contribute to a better understanding of chicken fat deposition.

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