scholarly journals Cytoprotective Effects and Mechanisms of Δ-17 Fatty Acid Desaturase in Injured Human Umbilical Vein Endothelial Cells (HUVECs)

2017 ◽  
Vol 23 ◽  
pp. 1627-1635 ◽  
Author(s):  
Haoyu Zhou ◽  
Chengming Wang
Keyword(s):  
Endothelial Cells ◽  
Fatty Acid ◽  
Umbilical Vein ◽  
Fatty Acid Desaturase ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Lipid Rafts Promote trans Fatty Acid-Induced Inflammation in Human Umbilical Vein Endothelial Cells

Lipids ◽  
2016 ◽  
Vol 52 (1) ◽  
pp. 27-35 ◽  
Author(s):  
Yao Pan ◽  
Benxin Liu ◽  
Zeyuan Deng ◽  
Yawei Fan ◽  
Jing Li ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Fatty Acid ◽  
Lipid Rafts ◽  
Trans Fatty Acid ◽  
Umbilical Vein ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

scholarly journals Enhancement of thrombin-thrombomodulin-catalysed protein C activation by phosphatidylethanolamine containing unsaturated fatty acids: possible physiological significance of phosphatidylethanolamine in anticoagulant activity of thrombomodulin

1994 ◽  
Vol 301 (3) ◽  
pp. 683-691 ◽  
Author(s):  
S Horie ◽  
H Ishii ◽  
H Hara ◽  
M Kazama
Keyword(s):  
Endothelial Cells ◽  
Plasma Membrane ◽  
Fatty Acid ◽  
Protein C ◽  
Unsaturated Fatty Acids ◽  
Umbilical Vein ◽  
Anticoagulant Activity ◽  
Dependent Manner ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

The effects of phospholipid vesicles and their fatty acid compositions on the acceleration of Protein C activation by thrombin-thrombomodulin was studied in vitro. Four main phospholipid fractions were prepared from cultured human umbilical vein endothelial cells, and purified thrombomodulin from human placenta was reconstituted into vesicles consisting of phosphatidylcholine (PtdCho) alone, PtdCho plus phosphatidylethanolamine (PtdEtn), PtdCho plus phosphatidylserine (PtdSer) and PtdCho plus PtdIns (1:1, w/w in each case). Vesicles of PtdCho, PtdIns/PtdCho, PtdSer/PtdCho and PtdEtn/PtdCho increased thrombin-thrombomodulin-catalysed protein C activation by 1.2-, 1.9-, 4.3- and 8.4-fold respectively compared with that in the absence of phospholipid. This Protein C activation was not affected by distearoyl PtdEtn/distearoyl PtdCho, whereas it was markedly increased with increasing content of unsaturated fatty acid in PtdEtn. The thrombin-dependent Protein C activation by thrombomodulin reconstituted into dilinolenoyl PtdEtn/distearoyl PtdCho was 14.6 times that by thrombomodulin reconstituted into distearoyl PtdEtn/distearoyl PtdCho, as a result of a decrease in the dissociation constant (Kd) for thrombin and the Michaelis constant (Km) for Protein C of thrombomodulin. Binding of Protein C to PtdEtn/PtdCho fixed to a microwell plate required the presence of CaCl2 and increased with increasing degree of unsaturation of fatty acid in PtdEtn. As PtdEtn appeared on the outside of the plasma membrane in cultured human umbilical vein endothelial cells after thrombin stimulation, it was presumed that Protein C activation could be elevated by PtdEtn at the outer surface of the plasma membrane via an increased affinity between thrombomodulin, thrombin and Protein C, resulting from both increased formation of the thrombin-thrombomodulin complex via a conformational change in thrombomodulin and increased binding of Protein C to the membrane phospholipid in a Ca(2+)-dependent manner.

scholarly journals 10-Hydroxy-2-decenoic Acid, a Major Fatty Acid from Royal Jelly, Inhibits VEGF-Induced Angiogenesis in Human Umbilical Vein Endothelial Cells

2009 ◽  
Vol 6 (4) ◽  
pp. 489-494 ◽  
Author(s):  
Hiroshi Izuta ◽  
Yuichi Chikaraishi ◽  
Masamitsu Shimazawa ◽  
Satoshi Mishima ◽  
Hideaki Hara
Keyword(s):  
Endothelial Cells ◽  
Fatty Acid ◽  
Royal Jelly ◽  
Umbilical Vein ◽  
Major Fatty Acid ◽  
Tube Formation ◽  
Angiogenic Factor ◽  
Decenoic Acid ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

Vascular endothelial growth factor (VEGF) is reported to be a potent pro-angiogenic factor that plays a pivotal role in both physiological and pathological angiogenesis. Royal jelly (RJ) is a honeybee product containing various proteins, sugars, lipids, vitamins and free amino acids. 10-Hydroxy-2-decenoic acid (10HDA), a major fatty acid component of RJ, is known to have various pharmacological effects; its antitumor activity being especially noteworthy. However, the mechanism underlying this effect is unclear. We examined the effect of 10HDA on VEGF-induced proliferation, migration and tube formation in human umbilical vein endothelial cells (HUVECs). Our findings showed that, 10HDA at 20 µM or more significantly inhibited such proliferation, migration and tube formation. Similarly, 10 µM GM6001, a matrix metalloprotease inhibitor, prevented VEGF-induced migration and tube formation. These findings indicate that 10HDA exerts an inhibitory effect on VEGF-induced angiogenesis, partly by inhibiting both cell proliferation and migration. Further experiments will be needed to clarify the detailed mechanism.

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