scholarly journals Expression of Serum Sialic Acid, Early Antigen-IgA, and Viral Capsid Antigen-IgA in Nasopharynx Cancer Patients: The Diagnostic Implication of Combined Assays

2015 ◽  
Vol 21 ◽  
pp. 4068-4073
Author(s):  
Yuning Sun ◽  
Caibo Sun ◽  
Endong Zhang
Keyword(s):  
Sialic Acid ◽  
Cancer Patients ◽  
Viral Capsid ◽  
Viral Capsid Antigen ◽  
Early Antigen ◽  
Serum Sialic Acid ◽  
Nasopharynx Cancer

Infectious antibodies in systemic lupus erythematosus patients

Lupus ◽  
2009 ◽  
Vol 18 (13) ◽  
pp. 1129-1135 ◽  
Author(s):  
Y. Berkun ◽  
G. Zandman-Goddard ◽  
O. Barzilai ◽  
M. Boaz ◽  
Y. Sherer ◽  
...  
Keyword(s):  
Lupus Erythematosus ◽  
Epstein Barr Virus ◽  
Viral Capsid ◽  
Infectious Agents ◽  
Viral Capsid Antigen ◽  
Igg Antibodies ◽  
Systemic Lupus ◽  
Early Antigen ◽  
Barr Virus ◽  
Epstein Barr

Infections can act as environmental triggers that induce or promote systemic lupus erythematosus (SLE) in genetically predisposed individuals. New technologies, developed recently, enable simultaneous assessment of multiple antibodies. Antibodies to specific infectious agents may shed light into the mechanisms of induction of SLE. The aim of this study was to investigate the prevalence of seropositivity and the titers of antibodies to bacterial, viral, and parasitic agents in SLE patients compared with non-autoimmune controls. Sera from 260 individuals (120 SLE patients and 140 controls) were tested by the BioPlex 2200 Multiplexed Immunoassay method (BioRad) for the prevalence and titers of antibodies to eight infectious agents (Epstein—Barr virus: early antigen IgG, nuclear antigen IgG, viral capsid antigen IgG and IgM, heterophile IgM; cytomegalovirus IgG and IgM; Toxoplasma gondii IgG and IgM; rubella IgG and IgM; Treponema pallidum TPr15G, TPr17G, TPr47G; herpes simplex virus type 1 and 2 IgG; hepatitis C virus and hepatitis B core antibodies. Cytomegalovirus IgM and Epstein—Barr virus early antigen IgG (but not other Epstein—Barr virus antigens) were significantly more prevalent in SLE patients than in controls. Conversely, positive titers of hepatitis B core and rubella IgG antibodies were less prevalent in the SLE patients than in controls. Other differences in titer positivity prevalence were not detected between patients and controls. The titers of the cytomegalovirus IgM, Toxoplasma IgG, Epstein—Barr virus early antigen, and viral capsid antigen IgG antibodies were significantly higher in SLE compared with controls. Our data suggest the importance of previous exposure to infectious agents in the induction and the prevention of SLE. Lupus (2009) 18, 1129—1135.

scholarly journals CANCER PATIENTS

2006 ◽  
Vol 13 (03) ◽  
pp. 344-348
Author(s):  
MOHAMMAD JAWAID SABZWARI ◽  
Muhammad Riaz ◽  
MUKHTAR AHMAD ◽  
Muhammad Umair ◽  
MUHAMMAD TAHIR MAJEED
Keyword(s):  
Sialic Acid ◽  
Carcinoembryonic Antigen ◽  
Cancer Patients ◽  
Tumor Markers ◽  
Age Distribution ◽  
Laboratory Diagnosis ◽  
Cancer Management ◽  
Serum Sialic Acid ◽  
Diagnosis Of Cancer ◽  
Diagnostic Indicator

In the world cancer is one of the leading causes of death. Most cancerswhen detected early, can be potentially cured. Thus early laboratory diagnosis of cancer has great importance inmanagement. The tumor markers are one of the methods of cancer management in use today. Objectives: Tocompare serum sialic acid (SA) and carcinoembryonic antigen (CEA) as tumor markers in different cancer patients.Study Design: Prospective study Setting: Shaikh Zayed Medical Complex, Lahore. Material and Methods: Onehundred and fifty three (153) documented different cancer patients were studied to compare between serum sialic acidand carcinoembryonic antigen as a tumor marker along with fifty controls of matched sex and age distribution between30-85 years. The sialic acid was estimated by colorimetric procedure and carcinoembryonic antigen by EnzymeImmunoassay. Results: The results revealed that mean levels of sialic acid and CEA in cancer patients weresignificantly higher (p<0.05) as compared to controls, considering all malignancies together, or carcinoma,hematological malignancies and sarcoma alone. Statistical analysis showed sialic acid to be more sensitive (p<0.05)than CEA in detection of cancer. Conclusion: Sialic acid was seen to be a good diagnostic indicator as compared toCEA in most cancer patients.

Primary Epstein-Barr Virus Infections in Children

PEDIATRICS ◽  
1977 ◽  
Vol 59 (1) ◽  
pp. 16-21
Author(s):  
Ciro Valent Sumaya
Keyword(s):  
Epstein Barr Virus ◽  
Viral Capsid ◽  
Viral Capsid Antigen ◽  
Virus Infections ◽  
Early Antigen ◽  
Barr Virus ◽  
Ebv Infection ◽  
Antibody Titers ◽  
Epstein Barr ◽  
A Current

During a seroepidemiologic survey of a community, 13 (6.2%) of 209 children were found to be experiencing a current or recent primary Epstein-Barr virus (EBV) infection. The sera contained elevated antibody titers to viral capsid antigen of EBV, antibodies to early antigen (EA) of EBV, and specific IgM. The frequency of primary infections was highest in the first decade of life. The primary EBV infections were usually asymptomatic. The antibody to EA was directed predominantly to the R component. A heterophil antibody response was not detected.

Serum Sialic Acid Levels in Lung Cancer Patients

Pharmacology ◽  
1976 ◽  
Vol 14 (1) ◽  
pp. 47-51 ◽  
Author(s):  
F.J. Krolikowski ◽  
K. Reuter ◽  
T.P. Waalkes ◽  
S.M. Sieber ◽  
R.H. Adamson
Keyword(s):  
Lung Cancer ◽  
Sialic Acid ◽  
Cancer Patients ◽  
Lung Cancer Patients ◽  
Serum Sialic Acid

scholarly journals Uji serologi IgA karakter KNF EBNA1+VCA p-18 pada penderita keluhan kronis kepala leher

2011 ◽  
Vol 41 (2) ◽  
pp. 105
Author(s):  
Camelia Herdini ◽  
Susanna Hutajulu ◽  
Sagung Rai Indrasari ◽  
Bambang Hariwiyanto ◽  
Jajah Fachiro ◽  
...  
Keyword(s):  
Head And Neck ◽  
Epstein Barr Virus ◽  
Viral Capsid ◽  
Viral Capsid Antigen ◽  
Early Antigen ◽  
Barr Virus ◽  
Ebv Infection ◽  
Serology Test ◽  
Epstein Barr ◽  
High Level

Background: Nasopharyngeal carcinoma (NPC), especially the WHO type III, is correlated almost100% with Epstein Barr Virus (EBV) infection. This is indicated by high IgG and IgA antibody responsesagainst viral capsid antigen (VCA), early antigen (EA) and Epstein Barr Nuclear antigen (EBNA).Increased IgA NPC character antibodies may be detected 2-10 years before the presence of the tumor. Thisoccurs as a result of reactivation of EBV infection. Purpose: To find out the level of IgA NPC characterantibodies (EBNA1+VCA p-18) in patients with chronic symptoms in the head and neck and to determine whether the level of IgA can be used as an early sign of NPC. Methods: Observational analytic study on 218 patients with chronic symptoms in the head and neck. The research was conducted from July 2006to September 2010. ELISA technique was used as serology test for IgA (EBNA1+VCA p-18). Result: Samples were 90 males and 128 females. High level of IgA by ELISA was found in 28 males (31.1%) and 45 females (35.2%). The IgA level tended to increase with age. The most common chronic symptoms inthe head and neck were chronic rhinitis (15.6%) and nasal obstruction (7.8%). From all patients who hadhigh level of IgA, 3 patients (4.1%) were found positive of early stage NPC. Conclusion: More than 33%of patients with chronic symptoms of head and neck had high level of IgA NPC character. This methodcan be used as an early detection of NPC. Keywords: serology test in NPC, EBNA1, VCA p-18, NPC symptoms in head and neck Abstrak :  Latar belakang: Karsinoma nasofaring (KNF) terutama tipe WHO III berkorelasi hampir 100%dengan infeksi Epstein Barr Virus (EBV). Hal ini ditunjukkan dengan tingginya respons antibodi IgGdan IgA terhadap viral capsid antigen (VCA), early antigen (EA) EBV serta antibodi Epstein BarrNuclear Antigen (EBNA). Kenaikan antibodi IgA dengan karakter KNF dapat terjadi 2-10 tahun sebelumterjadinya tumor. Hal ini terjadi sebagai akibat adanya reaktivasi infeksi EBV. Tujuan: Mengetahui kadarIgA karakter KNF (EBNA1+VCA p-18) pada penderita dengan gejala kronis di daerah kepala dan leherdan mengetahui apakah kadar IgA dapat digunakan sebagai tanda awal terjadinya KNF. Metode: Suatukajian analitik observasional terhadap 218 penderita dengan gejala kronis di daerah kepala dan leher.Penelitian ini dilakukan Juli 2006 sampai dengan September 2010. Pemeriksaan serologi IgA (EBNA1+VCA-p18)dilakukan denganteknik ELISA.Hasil:Terdapat90penderita laki-lakidan128 penderitaperempuan.HasiltesserologiIgAELISAdengankadartinggiditemukanpada28laki-laki(31,1%)dan45perempuan (35,2%). Kadar IgA cenderung meningkat pada peningkatan usia. Gejala kronis yangterbanyak dikeluhkan penderita adalah rinitis kronis, yaitu sebanyak 34 penderita (15,6%), diikuti denganobstruksi hidung sebanyak 17 penderita (7,8%). Pemeriksaan klinis lebih lanjut dari penderita yangmempunyai kadar IgA tinggi menunjukkan bahwa 3 penderita (4,1%) positif terkena kanker nasofaringstadium awal. __ Lebih dari 33% penderita dengan gejala kronis di daerah kepala dan lehermemiliki kadar IgA karakter KNF yang tinggi. Kadar IgA karakter KNF yang tinggi dapat digunakansebagai penanda awal kejadian KNF. Kata kunci: uji serologi KNF,EBNA1, VCA p-18, gejala KNF

Usefulness and Potential Pitfalls of Sialic Acid Determination in Sera of Patients with Ovarian Tumors

1986 ◽  
Vol 1 (1) ◽  
pp. 39-46 ◽  
Author(s):  
Bruno Berra ◽  
Silvana Rapelli ◽  
Gianluigi Monticelli ◽  
Maria Albertina Fighetti ◽  
Ines Della Mea ◽  
...  
Keyword(s):  
Sialic Acid ◽  
Tumor Marker ◽  
Cancer Patients ◽  
Preliminary Data ◽  
Ovarian Tumors ◽  
Serum Sialic Acid ◽  
Methodological Errors ◽  
Acid Determination

Increasing evidence in the literature indicates that serum sialic acid is increased in cancer patients suggesting a possible usefulness of its determination as a tumor marker. However there are many discrepancies in the data reported, probably due to methodological errors, mainly in lipid bound sialic measurement. In this paper we illustrate the results obtained when we applied a method worked out in our laboratory for the determination of total and fractionated sialic acid (lipid and protein bound) to the analysis of sera from patients with ovarian tumors and the preliminary data on the follow up of selected cases. The potential pitfalls in using this relatively new tumor marker will be critically evaluated.

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