scholarly journals Proteomic Profiling of Aging in Glomeruli of Mice by using Two-Dimensional Differential Gel Electrophoresis

2015 ◽  
Vol 21 ◽  
pp. 419-425 ◽  
Author(s):  
Lining Wang
Keyword(s):  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Proteomic Profiling ◽  
Differential Gel Electrophoresis

scholarly journals Proteomic Profiling and Neurodegeneration in West-Nile-Virus-Infected Neurons

2005 ◽  
Vol 2005 (3) ◽  
pp. 271-279 ◽  
Author(s):  
V. Dhingra ◽  
Q. Li ◽  
A. B. Allison ◽  
D. E. Stallknecht ◽  
Z. F. Fu
Keyword(s):  
Mass Spectrometry ◽  
West Nile Virus ◽  
Gel Electrophoresis ◽  
Fluorescent Dyes ◽  
Two Dimensional ◽  
Proteomic Profiling ◽  
West Nile ◽  
Altered Expression ◽  
Peptide Mass ◽  
Differential Gel Electrophoresis

West Nile virus, a mosquito-borne flavivirus, is a human, equine, and avian pathogen. High-resolution two-dimensional differential-gel electrophoresis (2D-DIGE) was used to characterize protein expression in primary rat neurons and to examine the proteomic profiling to understand the pathogenesis of West-Nile-associated meningoencephalitis. Three pH ranges, 3–10, 4–7, and 5–6, were used to analyze the protein spots. The proteins are labeled with fluorescent dyes Cy3 and Cy5 before being separated on the basis of charge and size respectively on a two-dimensional platform. About 55 proteins showed altered expression levels. These were then subsequently digested and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis using peptide mass fingerprinting and database searching. These cellular proteins could represent distinct roles during infection related to apoptosis. Our findings show that two-dimensional differential gel electrophoresis combined with mass spectrometry is a powerful approach that permits the identification of proteins whose expression was altered due to West Nile virus infection.

scholarly journals Proteomics Analysis by Two-Dimensional Differential Gel Electrophoresis Reveals the Lack of a Broad Response of Neisseria meningitidis to In Vitro-Produced AI-2

2005 ◽  
Vol 187 (1) ◽  
pp. 392-395 ◽  
Author(s):  
Stephan Schauder ◽  
Lucia Penna ◽  
Adeline Ritton ◽  
Catherine Manin ◽  
Fabienne Parker ◽  
...  
Keyword(s):  
Protein Expression ◽  
Neisseria Meningitidis ◽  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Differential Protein Expression ◽  
Proteomics Analysis ◽  
Global Response ◽  
Differential Gel Electrophoresis ◽  
Signaling Activity

ABSTRACT To investigate the effect of the autoinducer AI-2 on protein expression in Neisseria meningitidis, a luxS mutant of strain MC58 was grown in the presence and absence of in vitro-produced AI-2, and differential protein expression was assessed by two-dimensional differential gel electrophoresis. N. meningitidis did not show a global response to AI-2 signaling activity.

Validation and development of fluorescence two-dimensional differential gel electrophoresis proteomics technology

PROTEOMICS ◽  
2001 ◽  
Vol 1 (3) ◽  
pp. 377-396 ◽  
Author(s):  
Robert Tonge ◽  
Joanne Shaw ◽  
Brian Middleton ◽  
Rachel Rowlinson ◽  
Steve Rayner ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Proteomics Technology ◽  
Differential Gel Electrophoresis

Proteomic profiling of dog urine after European adder (Vipera berus berus) envenomation by two-dimensional difference gel electrophoresis

Toxicon ◽  
2012 ◽  
Vol 60 (7) ◽  
pp. 1228-1234 ◽  
Author(s):  
Mari Palviainen ◽  
Marja Raekallio ◽  
Mari Vainionpää ◽  
Suvi Kosonen ◽  
Outi Vainio
Keyword(s):  
Gel Electrophoresis ◽  
Two Dimensional ◽  
Proteomic Profiling ◽  
Vipera Berus ◽  
Difference Gel Electrophoresis
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