Cytomegalovirus (CMV) Monitoring After Liver Transplantation: Comparison of CMV Pp65 Antigenemia Assay with Real-Time PCR Calibrated to WHO International Standard

2016 ◽  
Vol 21 ◽  
pp. 131-136 ◽  
Author(s):  
Hideya Kamei ◽  
Yoshinori Ito ◽  
Yasuharu Onishi ◽  
Michio Suzuki ◽  
Hisashi Imai ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Real Time ◽  
Real Time Pcr ◽  
International Standard ◽  
Antigenemia Assay ◽  
Pp65 Antigenemia

scholarly journals Pp65 antigenemia, plasma real-time PCR and DBS test in symptomatic and asymptomatic cytomegalovirus congenitally infected newborns

2010 ◽  
Vol 10 (1) ◽  
Author(s):  
Sandro Binda ◽  
Antonella Mammoliti ◽  
Valeria Primache ◽  
Patrizia Didò ◽  
Carlo Corbetta ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pp65 Antigenemia

Comparison of a UL111a real-time PCR and pp65 antigenemia for the detection of cytomegalovirus

2011 ◽  
Vol 83 (12) ◽  
pp. 2143-2150 ◽  
Author(s):  
Christiane Beckmann ◽  
Alexis Dumoulin ◽  
Christine Hanssen Rinaldo ◽  
Hans H. Hirsch
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pp65 Antigenemia

Enhanced enrichment and detection of thermotolerant Campylobacter species from water using the Portable Microbe Enrichment Unit and real-time PCR

2009 ◽  
Vol 55 (7) ◽  
pp. 849-858 ◽  
Author(s):  
Tarja Pitkänen ◽  
Juliane Bräcker ◽  
Ilkka T. Miettinen ◽  
Anneli Heitto ◽  
Jouni Pesola ◽  
...  
Keyword(s):  
Drinking Water ◽  
Real Time ◽  
Campylobacter Jejuni ◽  
Real Time Pcr ◽  
Pcr Detection ◽  
Detection Time ◽  
The Real ◽  
International Standard ◽  
Campylobacter Species

An enhanced enrichment using the Portable Microbe Enrichment Unit (PMEU) with the microaerobic bubbling of broths was applied for the detection of thermotolerant Campylobacter species from water. This PMEU enrichment was compared with the conventional static enrichment of the international standard ISO 17995:2005. In addition, Campylobacter detection after enrichment using a real-time PCR detection was compared with colony counts. The tests with stressed Campylobacter jejuni cells in drinking water indicated that the PMEU enrichment yielded a significantly higher number of Campylobacter cells in the Bolton broth compared with the conventional static incubation. Application of the real-time PCR technique shortened the Campylobacter detection time. This combination of method modifications can be used for Campylobacter detection from water and adds methodological repertoire for the rapid survey and management of waterborne outbreaks.

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