scholarly journals Effect of Elevated CO2 Concentration, Elevated Temperature and No Nitrogen Fertilization on Methanogenic Archaeal and Methane-Oxidizing Bacterial Community Structures in Paddy Soil

2016 ◽  
Vol 31 (3) ◽  
pp. 349-356 ◽  
Author(s):  
Dongyan Liu ◽  
Kanako Tago ◽  
Masahito Hayatsu ◽  
Takeshi Tokida ◽  
Hidemitsu Sakai ◽  
...  
Keyword(s):  
Elevated Temperature ◽  
Bacterial Community ◽  
Paddy Soil ◽  
Nitrogen Fertilization ◽  
Community Structures

scholarly journals Warming shapes nirS- and nosZ-type denitrifier communities and stimulates N2O emission in acidic paddy soil

2021 ◽  
Author(s):  
Xiaoyi Xing ◽  
Yafang Tang ◽  
Huifang Xu ◽  
Hongling Qin ◽  
Yi Liu ◽  
...  
Keyword(s):  
Global Warming ◽  
Elevated Temperature ◽  
Paddy Soil ◽  
High Throughput Sequencing ◽  
Pore Space ◽  
Paddy Soils ◽  
Community Structures ◽  
Total N ◽  
Temperature Range ◽  
Denitrifier Communities

Warming strongly stimulates soil nitrous oxide (N2O) emission, contributing to the global warming trend. Submerged paddy soils exhibit huge N2O emission potential; however, the N2O emission pathway and underlying mechanisms under warming are not clearly understood. We conducted an incubation experiment using 15N to investigate the dynamics of N2O emission at controlled temperatures (5, 15, 25, and 35 °C) in 125% water-filled pore space. The community structures of nitrifiers and denitrifiers were determined via high-throughput sequencing of functional genes. Our results showed that elevated temperature sharply enhanced soil N2O emission from submerged paddy soil. Denitrification was the main contributor, accounting for more than 90% of total N2O emission at all treatment temperatures. N2O flux was coordinatively regulated by nirK-, nirS-, and nosZ-containing denitrifiers, but not ammonia-oxidising archaea or ammonia-oxidising bacteria. The nirS-containing denitrifiers were more sensitive to temperature shifts, especially at a lower temperature range (5 to 25 °C), and showed a stronger correlation with N2O flux than that of nirK-containing denitrifiers. By contrast, nosZ-containing denitrifiers exhibited substantial variation at higher temperatures (15 to 35 °C), thereby playing an important role in N2O consumption. Certain taxa of nirS- and nosZ-containing denitrifiers regulated N2O flux, including nirS-containing denitrifiers affiliated with Rhodanbacter and Cupriavidus as well as nosZ-containing denitrifiers affiliated with Azoarcus and Azospirillum. Together, these findings suggest that elevated temperature can significantly increase N2O emission from denitrification in submerged paddy soils by shifting the overall community structures and enriching some indigenous taxa of nirS- and nosZ-containing denitrifiers. Importance The interdependence between global warming and greenhouse gas N2O has always been the hotspot. However, information on factors contributing to N2O and temperature-dependent community structure changes are scarce. This study demonstrated high temperature-induced N2O emission from submerged paddy soils, mainly via stimulating denitrification. Further, we speculate that key functional denitrifiers drive N2O emission. This study showed that denitrifiers were more sensitive to temperature rise than nitrifiers, and the temperature sensitivity differed among denitrifier communities. N2O-consuming denitrifiers (nosZ-containing denitrifiers) were more sensitive at a higher temperature range than N2O-producing denitrifiers (nirS-containing denitrifiers). This study’s findings help predict N2O fluxes under different degrees of warming and develop strategies to mitigate N2O emissions from paddy fields based on microbial community regulation.

Long-term nitrogen fertilization impacts soil fungal and bacterial community structures in a dryland soil of Loess Plateau in China

2017 ◽  
Vol 18 (4) ◽  
pp. 1632-1640 ◽  
Author(s):  
Jinshan Liu ◽  
Xiang Zhang ◽  
Hui Wang ◽  
Xiaoli Hui ◽  
Zhaohui Wang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Loess Plateau ◽  
Nitrogen Fertilization ◽  
Community Structures

Responses of nitrification and bacterial community in three size aggregates of paddy soil to both of initial fertility and biochar addition

2021 ◽  
Vol 166 ◽  
pp. 104004
Author(s):  
Qiong Hou ◽  
Ting Zuo ◽  
Jian Wang ◽  
Shan Huang ◽  
Xiaojun Wang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Paddy Soil

scholarly journals Microbial Population Changes during Bioremediation of an Experimental Oil Spill

1999 ◽  
Vol 65 (8) ◽  
pp. 3566-3574 ◽  
Author(s):  
Sarah J. MacNaughton ◽  
John R. Stephen ◽  
Albert D. Venosa ◽  
Gregory A. Davis ◽  
Yun-Juan Chang ◽  
...  
Keyword(s):  
Microbial Community ◽  
Bacterial Community ◽  
Oil Spill ◽  
Phospholipid Fatty Acid ◽  
Community Structures ◽  
Gram Negative ◽  
Dgge Analysis ◽  
16S Rdna Pcr ◽  
Microbial Community Structures ◽  
Plfa Analysis

ABSTRACT Three crude oil bioremediation techniques were applied in a randomized block field experiment simulating a coastal oil spill. Four treatments (no oil control, oil alone, oil plus nutrients, and oil plus nutrients plus an indigenous inoculum) were applied. In situ microbial community structures were monitored by phospholipid fatty acid (PLFA) analysis and 16S rDNA PCR-denaturing gradient gel electrophoresis (DGGE) to (i) identify the bacterial community members responsible for the decontamination of the site and (ii) define an end point for the removal of the hydrocarbon substrate. The results of PLFA analysis demonstrated a community shift in all plots from primarily eukaryotic biomass to gram-negative bacterial biomass with time. PLFA profiles from the oiled plots suggested increased gram-negative biomass and adaptation to metabolic stress compared to unoiled controls. DGGE analysis of untreated control plots revealed a simple, dynamic dominant population structure throughout the experiment. This banding pattern disappeared in all oiled plots, indicating that the structure and diversity of the dominant bacterial community changed substantially. No consistent differences were detected between nutrient-amended and indigenous inoculum-treated plots, but both differed from the oil-only plots. Prominent bands were excised for sequence analysis and indicated that oil treatment encouraged the growth of gram-negative microorganisms within the α-proteobacteria andFlexibacter-Cytophaga-Bacteroides phylum. α-Proteobacteria were never detected in unoiled controls. PLFA analysis indicated that by week 14 the microbial community structures of the oiled plots were becoming similar to those of the unoiled controls from the same time point, but DGGE analysis suggested that major differences in the bacterial communities remained.

Microbial communities in paddy soil as influenced by nitrogen fertilization and water regimes

2021 ◽  
Author(s):  
Yan Sun ◽  
Yichen Wang ◽  
Zhi Qu ◽  
Weiyi Mu ◽  
Wenhai Mi ◽  
...  
Keyword(s):  
Microbial Communities ◽  
Paddy Soil ◽  
Nitrogen Fertilization ◽  
Water Regimes

Response of activity, abundance, and composition of anammox bacterial community to different fertilization in a paddy soil

2018 ◽  
Vol 54 (8) ◽  
pp. 977-984 ◽  
Author(s):  
San’an Nie ◽  
Xiumei Lei ◽  
Lixia Zhao ◽  
Yi Wang ◽  
Fei Wang ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Paddy Soil

scholarly journals Bacterial community structures are unique and resilient in full-scale bioenergy systems

2011 ◽  
Vol 108 (10) ◽  
pp. 4158-4163 ◽  
Author(s):  
J. J. Werner ◽  
D. Knights ◽  
M. L. Garcia ◽  
N. B. Scalfone ◽  
S. Smith ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Full Scale ◽  
Community Structures ◽  
Bioenergy Systems

scholarly journals Changes in Denitrification Potentials and Riverbank Soil Bacterial Structures along Shibetsu River, Japan

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Yoshitaka Uchida ◽  
Hirosato Mogi ◽  
Toru Hamamoto ◽  
Miwako Nagane ◽  
Misato Toda ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Inorganic Nitrogen ◽  
Anaerobic Incubation ◽  
Community Structures ◽  
Bacterial Response ◽  
Glucose Carbon ◽  
Soil Ecosystems ◽  
Increase Rate ◽  
Before And After ◽  
C And N

Riverbank soil ecosystems are important zones in terms of transforming inorganic nitrogen (N), particularly nitrate (NO3−-N), in soils to nitrous oxide (N2O) gases. Thus, the gasification of N in the riverbank soil ecosystems may produce a greenhouse gas, N2O, when the condition is favourable for N2O-producing microbes. One of the major N2O-producing pathways is denitrification. Thus, we investigated the denitrification potentials along Shibetsu River, Hokkaido, Japan. We sampled riverbank soils from eight sites along the Shibetsu River. Their denitrification potentials with added glucose-carbon (C) and NO3−-N varied from 4.73 to 181 μg·N·kg−1·h−1. The increase of the denitrification after the addition of C and N was negatively controlled by soil pH and positively controlled by soil NH4+-N levels. Then, we investigated the changes in 16S rRNA bacterial community structures before and after an anaerobic incubation with added C and N. We investigated the changes in bacterial community structures, aiming to identify specific microbial species related to high denitrification potentials. The genus Gammaproteobacteria AeromonadaceaeTolumonaswas markedly increased, from 0.0 ± 0.0% to 16 ± 17%, before and after the anaerobic incubation with the excess substrates, when averaged across all the sites. Although we could not find a significant interaction between the denitrification potential and the increase rate of G. AeromonadaceaeTolumonas, our study suggested that along the Shibetsu River, bacterial response to added excess substrates was similar at the genus level. Further studies are needed to investigate whether this is a universal phenomenon even in other rivers.

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