scholarly journals Infection, Multiplication and Evaluation of the Nitrogen-Fixing Ability of Herbaspirillum sp. Strain B501gfp1 in Sugarcane Stems Inoculated by the Vacuum Infiltration Method

2008 ◽  
Vol 23 (2) ◽  
pp. 128-133 ◽  
Author(s):  
Muhammad Zakria ◽  
Kana Udonishi ◽  
Yuichi Saeki ◽  
Akihiro Yamamoto ◽  
Shoichiro Akao
Keyword(s):  
Vacuum Infiltration ◽  
Nitrogen Fixing ◽  
Infiltration Method

scholarly journals Enhanced Histological Technique for Observation of Spathe Pigmentation in Anthurium species and Hybrids

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 865D-865
Author(s):  
Jennifer A. Ehrenberger* ◽  
Adelheid R. Kuehnle
Keyword(s):  
Polyethylene Glycol ◽  
Cross Sections ◽  
Spatial Localization ◽  
Vacuum Infiltration ◽  
New Method ◽  
Color Intensity ◽  
Histological Technique ◽  
Parental Material ◽  
The Cross ◽  
Infiltration Method

A hybridization strategy for certain coloration could be developed based on accurate histological information of parental material together with the knowledge of heritability of color and color intensity. A sample of 12 Anthurium species and hybrids were histologically examined for pigmentation in spathes using a new method employing vacuum infiltration of spathe tissue with polyethylene glycol (PEG) prior to cross-sectioning. PEG infiltration displaces intercellular air spaces between cells. This method greatly improved the clarity of the cross sections and consequently improved observations of spatial localization of anthocyanins and chloroplasts. This infiltration method accurately identified the spatial localization of pigments for future breeding reference, notably among Anthurium species.

SYNTHESIS AND HYDROLYSIS OF SUCROSE BY WHEAT LEAVES, AS DETERMINED BY THE VACUUM INFILTRATION METHOD

1952 ◽  
Vol 30 (1) ◽  
pp. 28-39 ◽  
Author(s):  
G. Krotkov ◽  
W. Constance G. Bennett
Keyword(s):  
Progressive Increase ◽  
Sodium Cyanide ◽  
Vacuum Infiltration ◽  
Free Sugar ◽  
Diurnal Changes ◽  
Sucrose Synthesis ◽  
Rate Of Hydrolysis ◽  
Wheat Leaves ◽  
Hydrolysis Of ◽  
Infiltration Method

Using the technique of vacuum infiltration, either a mixture of glucose plus fructose or a solution of sucrose alone was introduced into detached wheat leaves, and the rates of sucrose synthesis or hydrolysis from these infiltrated sugars were observed. It was found that these rates were not constant during 24 hr., but depended on the time of the day or night when the leaves were cut. Synthesis was high in leaves detached in the forenoon, before sunset, and in the first part of the night. Changes in the rate of hydrolysis were usually mirror images of those of synthesis. It has been concluded that in wheat leaves there exists a diurnal rhythm in their synthetic and hydrolytic potential and that the observed diurnal changes in the rates of sucrose synthesis and hydrolysis represent one of the manifestations of such a rhythm. Sodium fluoride, sodium cyanide, iodoacetate, and dinitrophenol always increased hydrolysis, and usually decreased synthesis. When glucose-1-phosphate, fructose-6-phosphate, and fructose-1, 6-diphosphate were substituted for the corresponding free sugar in a mixture of glucose plus fructose, a decrease in sucrose synthesis was observed. After 24 hr. of starvation the rate of sucrose synthesis declined, and eventually dropped practically to zero at the end of five days. In the same time there was a progressive increase in the rate of hydrolysis.

scholarly journals Screening of a multi-virus resistant RNAi construct in cowpea through transient vacuum infiltration method

VirusDisease ◽  
2019 ◽  
Vol 30 (2) ◽  
pp. 269-278
Author(s):  
K. Prasad Babu ◽  
Manamohan Maligeppagol ◽  
R. Asokan ◽  
M. Krishna Reddy
Keyword(s):  
Vacuum Infiltration ◽  
Rnai Construct ◽  
Infiltration Method

scholarly journals Construction of Full-length Infectious cDNA Clones of Apple chlorotic leaf spot virus and Their Agroinoculation to Woody Plants by a Novel Method of Vacuum Infiltration

Plant Disease ◽  
2017 ◽  
Vol 101 (12) ◽  
pp. 2110-2115 ◽  
Author(s):  
Lei Zhang ◽  
Wilhelm Jelkmann
Keyword(s):  
Leaf Spot ◽  
Plant Viruses ◽  
Vacuum Infiltration ◽  
Full Length ◽  
Cdna Clones ◽  
Spot Virus ◽  
Full Length Cdna ◽  
Chlorotic Leaf ◽  
Multiple Strains ◽  
Infiltration Method

Construction and agroinoculation of full-length infectious cDNA clones of plant RNA viruses have been used in plant virology to prove Koch’s postulates and for development of viruses as vectors for expressing foreign genes in plants. Four full-length cDNA clones (pIF3-12, pIF3-14, pIF3-15, and pIF3-19) of Apple chlorotic leaf spot virus (ACLSV) isolate 38/85 were produced. Two of the four full-length cDNA clones (pIF3-15 and pIF3-19) proved to be infectious on Nicotiana occidentalis 37B test plants by agroinoculation and were then mechanically transmissible to healthy N. occidentalis 37B. The genomic cDNAs of ACLSV pIF3-15 and pIF3-19 shared nucleotide identity of 77.5%, demonstrating mixed infections of multiple strains of ACLSV in the source tree of isolate 38/85. The two full-length cDNA clones were agroinoculated to apple seedlings by a newly developed vacuum infiltration method. The success rate of agroinoculation was greater than 78%, defined as the number of PCR positive seedlings to the number of apple seedlings that survived. ACLSV was transmissible from agroinoculated seedlings by cleft grafting. The results of this study will be useful for construction of infectious cDNA clones of plant viruses from full-length PCR fragments and agroinoculating woody host plants using the vacuum infiltration method outlined here.

scholarly journals In planta transformation of buckwheat (Fagopyrum esculentum Moench.)

2007 ◽  
Vol 59 (2) ◽  
pp. 135-138 ◽  
Author(s):  
Ana Bratic ◽  
Dragana Majic ◽  
Jovanka Miljus-Djukic ◽  
Z.S. Jovanovic ◽  
Vesna Maksimovic
Keyword(s):  
Vacuum Infiltration ◽  
Fagopyrum Esculentum ◽  
Functional Study ◽  
In Planta Transformation ◽  
In Planta ◽  
Fagopyrum Esculentum Moench ◽  
Transformation Methods ◽  
Infiltration Method

In order to develop a reliable and rapid transgenic system for functional study of specific buckwheat gene constructs, two different in planta transformation methods were analyzed: vacuum infiltration and infiltration by syringe. The results indicated that the vacuum infiltration method was much more efficient and can therefore be considered the method of choice for buckwheat transformation. .

scholarly journals High aspect ratio lead zirconate titanate tube structures: I. Template assisted fabrication - vacuum infiltration method

2012 ◽  
Vol 6 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Vladimír Kovaľ
Keyword(s):  
Aspect Ratio ◽  
Lead Zirconate Titanate ◽  
High Aspect Ratio ◽  
Perovskite Phase ◽  
Pyrochlore Phase ◽  
Vacuum Infiltration ◽  
Memory Storage ◽  
Pure Oxygen ◽  
Outer Diameter ◽  
Infiltration Method

Polycrystalline Pb(Zr0.52Ti0.48 )O3 (PZT) microtubes are fabricated by a vacuum infiltration method. The method is based on repeated infiltration of precursor solution into macroporous silicon (Si) templates at a sub-atmospheric pressure. The pyrolyzed PZT tubes of a 2-?m outer diameter, extending to over 30 ?m in length were released from the template using a selective isotropic-pulsed XeF2 reactive ion etching of silicon. Free-standing microtubes, partially anchored at the bottom of the Si template, were then crystallized in pure oxygen atmosphere at 750 ?C for 2 min using a rapid thermal annealer. The perovskite phase of the final PZT tubes was confirmed by X-ray diffraction (XRD) analysis. The XRD spectrum also revealed a small amount of the pyrochlore phase in the structure and signs of possible fluoride contamination caused most likely by the XeF2 etching process. The surface morphology was examined using scanning electron microscopy. It was demonstrated that the whole surface of the pore walls was conformally coated during the repeated infiltration of templates, resulting in straight tubes with closed tips formed on the opposite ends as replicas of the pore bottoms. These high aspect ratio ferroelectric structures are suggested as building units for developing miniaturized electronic devices, such as memory storage (DRAM trenched) capacitors, piezoelectric scanners and actuators, and are of fundamental value for the theory of ferroelectricity in systems with low dimensionality.

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