scholarly journals Comprehensive Analysis of Cell Wall-Permeabilizing Conditions for Highly Sensitive Fluorescence In Situ Hybridization

2006 ◽  
Vol 21 (4) ◽  
pp. 227-234 ◽  
Author(s):  
Kazuhiro Furukawa ◽  
Tatsuhiko Hoshino ◽  
Satoshi Tsuneda ◽  
Yuhei Inamori
Keyword(s):  
Cell Wall ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Comprehensive Analysis ◽  
Highly Sensitive

A supersandwich fluorescence in situ hybridization strategy for highly sensitive and selective mRNA imaging in tumor cells

2016 ◽  
Vol 52 (2) ◽  
pp. 370-373 ◽  
Author(s):  
Jin Huang ◽  
He Wang ◽  
Xiaohai Yang ◽  
Yanjing Yang ◽  
Ke Quan ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Tumor Cells ◽  
Signal Amplification ◽  
Fluorescence Signal ◽  
Target Mrna ◽  
Highly Sensitive ◽  
Fluorescence Signal Amplification

This strategy uses two fluorophore-labeled signal probes to generate a supersandwich product, which in turn generates numerous signal probes located at the target mRNA position, resulting in thein situfluorescence signal amplification.

Highly sensitive analysis for N-myc amplification in neuroblastoma based on fluorescence in situ hybridization

1999 ◽  
Vol 34 (11) ◽  
pp. 1615-1619 ◽  
Author(s):  
Tatsurou Tajiri ◽  
Kumiko Shono ◽  
Yoshimitsu Fujii ◽  
Shinichi Noguchi ◽  
Yoshiaki Kinoshita ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Sensitive Analysis ◽  
Highly Sensitive

scholarly journals Comprehensive Analysis of ETS Family Members in Melanoma by Fluorescence In Situ Hybridization Reveals Recurrent ETV1 Amplification

2013 ◽  
Vol 6 (4) ◽  
pp. 405-412 ◽  
Author(s):  
Rohit Mehra ◽  
Saravana M. Dhanasekaran ◽  
Nallasivam Palanisamy ◽  
Pankaj Vats ◽  
Xuhong Cao ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Family Members ◽  
Comprehensive Analysis ◽  
Ets Family

l-DNA-tagged fluorescence in situ hybridization for highly sensitive imaging of RNAs in single cells

2020 ◽  
Vol 18 (40) ◽  
pp. 8084-8088
Author(s):  
Motoyuki Ogata ◽  
Gosuke Hayashi ◽  
Anri Ichiu ◽  
Akimitsu Okamoto
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Single Cells ◽  
Rna Detection ◽  
Pcr Product ◽  
Highly Sensitive

l-DNA tagged FISH (LT-FISH), including two-step hybridization processes with a l–d chimera oligonucleotide and a fluorescence-labeled PCR product tethering a l-DNA tag, has realized sensitive RNA detection in fixed cells.

scholarly journals Two Major Archaeal Pseudomurein Endoisopeptidases: PeiW and PeiP

Archaea ◽  
2010 ◽  
Vol 2010 ◽  
pp. 1-4 ◽  
Author(s):  
Ganesh Ram R. Visweswaran ◽  
Bauke W. Dijkstra ◽  
Jan Kok
Keyword(s):  
Cell Wall ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Dna Isolation ◽  
Permeability Ratio ◽  
Archaeal Species ◽  
Protoplast Preparation ◽  
Catalyzed Reporter Deposition ◽  
Card Fish

PeiW (UniProtKB Q7LYX0) and PeiP (UniProtKB Q77WJ4) are the two major pseudomurein endoisopeptidases (Pei) that are known to cleave pseudomurein cell-wall sacculi of the members of the methanogenic ordersMethanobacterialesandMethanopyrales. Both enzymes, originating from prophages specific for some methanogenic archaeal species, hydrolyze the (Ala)-Lys bond of the peptide linker between adjacent pseudomurein layers. Because lysozyme is not able to cleave the pseudomurein cell wall, the enzymes are used in protoplast preparation and in DNA isolation from pseudomurein cell-wall-containing methanogens. Moreover, PeiW increases the probe permeability ratio and enables fluorescence in situ hybridization (FISH) and catalyzed reporter deposition (CARD-) FISH experiments to be performed on these methanogens.

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