scholarly journals Occurrence of Diazotrophic Endophytes in Different Portions of Sweetpotato Stems

2004 ◽  
Vol 19 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Katsuki Adachi ◽  
Yasuhiro Takahata ◽  
Constancio A. Asis, Jr.
Keyword(s):  
Diazotrophic Endophytes

Diazotrophic Endophytes of Poplar and Willow for Growth Promotion of Rice Plants in Nitrogen-Limited Conditions

Crop Science ◽  
2015 ◽  
Vol 55 (4) ◽  
pp. 1765-1772 ◽  
Author(s):  
S. L. Kandel ◽  
N. Herschberger ◽  
S.H. Kim ◽  
S. L. Doty
Keyword(s):  
Growth Promotion ◽  
Rice Plants ◽  
Diazotrophic Endophytes

scholarly journals Population of Diazotrophic Endophytes in Stem Apoplast Solution of Sugarcane and Related Grass Species in Tanegashima, Japan

2003 ◽  
Vol 18 (3) ◽  
pp. 133-137 ◽  
Author(s):  
Constancio A. Asis, Jr. ◽  
Katsuki Adachi ◽  
Akira Sugimoto ◽  
Kunihiro Ujihara ◽  
Yoshifumi Terajima ◽  
...  
Keyword(s):  
Grass Species ◽  
Diazotrophic Endophytes

scholarly journals Plant Growth Promotion Diversity in Switchgrass-Colonizing, Diazotrophic Endophytes

2021 ◽  
Vol 12 ◽  
Author(s):  
Sara Gushgari-Doyle ◽  
Marcus Schicklberger ◽  
Yifan V. Li ◽  
Robert Walker ◽  
Romy Chakraborty
Keyword(s):  
Plant Growth ◽  
Panicum Virgatum ◽  
Growth Promotion ◽  
Pgp Traits ◽  
Isolation And Characterization ◽  
Plant Growth Promoting ◽  
Cultivation Methods ◽  
Diazotrophic Endophytes ◽  
Raoultella Terrigena

Endophytic nitrogen-fixing (diazotrophic) bacteria are essential members of the microbiome of switchgrass (Panicum virgatum), considered to be an important commodity crop in bioenergy production. While endophytic diazotrophs are known to provide fixed atmospheric nitrogen to their host plant, there are many other plant growth-promoting (PGP) capabilities of these organisms to be demonstrated. The diversity of PGP traits across different taxa of switchgrass-colonizing endophytes is understudied, yet critical for understanding endophytic function and improving cultivation methods of important commodity crops. Here, we present the isolation and characterization of three diazotrophic endophytes: Azospirillum agricola R1C, Klebsiella variicola F10Cl, and Raoultella terrigena R1Gly. Strains R1C and F10Cl were isolated from switchgrass and strain R1Gly, while isolated from tobacco, is demonstrated herein to colonize switchgrass. Each strain exhibited highly diverse genomic and phenotypic PGP capabilities. Strain F10Cl and R1Gly demonstrated the highest functional similarity, suggesting that, while endophyte community structure may vary widely based on host species, differences in functional diversity are not a clearly delineated. The results of this study advance our understanding of diazotrophic endophyte diversity, which will allow us to design robust strategies to improve cultivation methods of many economically important commodity crops.

scholarly journals In silico differential display of defense-related expressed sequence tags from sugarcane tissues infected with diazotrophic endophytes

2001 ◽  
Vol 24 (1-4) ◽  
pp. 103-111 ◽  
Author(s):  
Marcio R. Lambais
Keyword(s):  
Hierarchical Clustering ◽  
Expressed Sequence Tags ◽  
Expression Profiles ◽  
Expression Patterns ◽  
Cdna Libraries ◽  
A Genome ◽  
Wide Scale ◽  
Clustering Approach ◽  
Diazotrophic Endophytes ◽  
Expressed Sequence

The expression patterns of 277 sugarcane expressed sequence tags (EST)-contigs encoding putative defense-related (DR) proteins were evaluated using the Sugarcane EST database. The DR proteins evaluated included chitinases, beta-1,3-glucanases, phenylalanine ammonia-lyases, chalcone synthases, chalcone isomerases, isoflavone reductases, hydroxyproline-rich glycoproteins, proline-rich glycoproteins, peroxidases, catalases, superoxide dismutases, WRKY-like transcription factors and proteins involved in cell death control. Putative sugarcane WRKY proteins were compared and their phylogenetic relationships determined. A hierarchical clustering approach was used to identify DR ESTs with similar expression profiles in representative cDNA libraries. To identify DR ESTs differentially expressed in sugarcane tissues infected with Gluconacetobacter diazotrophicus or Herbaspirillum rubrisubalbicans, 179 putative DR EST-contigs expressed in non-infected tissues (leaves and roots) and/or infected tissues were selected and arrayed by similarity of their expression profiles. Changes in the expression levels of 124 putative DR EST-contigs, expressed in non-infected tissues, were evaluated in infected tissues. Approximately 42% of these EST-contigs showed no expression in infected tissues, whereas 15% and 3% showed more than 2-fold suppression in tissues infected with G. diazotrophicus or H. rubrisubalbicans, respectively. Approximately 14 and 8% of the DR EST-contigs evaluated showed more than 2-fold induction in tissues infected with G. diazotrophicus or H. rubrisubalbicans, respectively. The differential expression of clusters of DR genes may be important in the establishment of a compatible interaction between sugarcane and diazotrophic endophytes. It is suggested that the hierarchical clustering approach can be used on a genome-wide scale to identify genes likely involved in controlling plant-microorganism interactions.

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