scholarly journals Physiological State of Vibrio anguillarum, a Fish Pathogen, under Starved and Low-Osmotic Environments

2003 ◽  
Vol 18 (3) ◽  
pp. 160-166 ◽  
Author(s):  
Mitsuru Eguchi ◽  
Erina Fujiwara-Nagata ◽  
Nobukazu Miyamoto
Keyword(s):  
Physiological State ◽  
Vibrio Anguillarum ◽  
Fish Pathogen

ChemInform Abstract: Structure of Anguibactin (Ia), a Unique Plasmid-Related Bacterial Siderophore from the Fish Pathogen Vibrio anguillarum

ChemInform ◽  
1989 ◽  
Vol 20 (15) ◽  
Author(s):  
M. A. F. JALAL ◽  
M. B. HOSSAIN ◽  
D. VAN DER HELM ◽  
J. SANDERS-LOEHR ◽  
L. A. ACTIS ◽  
...  
Keyword(s):  
Vibrio Anguillarum ◽  
Fish Pathogen ◽  
Abstract Structure ◽  
Unique Plasmid

scholarly journals Inhibition of Vibrio anguillarum byPseudomonas fluorescens AH2, a Possible Probiotic Treatment of Fish

1999 ◽  
Vol 65 (3) ◽  
pp. 969-973 ◽  
Author(s):  
Lone Gram ◽  
Jette Melchiorsen ◽  
Bettina Spanggaard ◽  
Ingrid Huber ◽  
Torben F. Nielsen
Keyword(s):  
Iron Concentration ◽  
Vibrio Anguillarum ◽  
Fish Farming ◽  
Fish Pathogen ◽  
Probiotic Treatment ◽  
Treated Fish ◽  
Culture Supernatants ◽  
Probiotic Effect

ABSTRACT To study the possible use of probiotics in fish farming, we evaluated the in vitro and in vivo antagonism of antibacterial strainPseudomonas fluorescens strain AH2 against the fish-pathogenic bacterium Vibrio anguillarum. As iron is important in virulence and bacterial interactions, the effect ofP. fluorescens AH2 was studied under iron-rich and iron-limited conditions. Sterile-filtered culture supernatants from iron-limited P. fluorescens AH2 inhibited the growth ofV. anguillarum, whereas sterile-filtered supernatants from iron-replete cultures of P. fluorescens AH2 did not.P. fluorescens AH2 inhibited the growth of V. anguillarum during coculture, independently of the iron concentration, when the initial count of the antagonist was 100 to 1,000 times greater that of the fish pathogen. These in vitro results were successfully repeated in vivo. A probiotic effect in vivo was tested by exposing rainbow trout (Oncorynchus mykissWalbaum) to P. fluorescens AH2 at a density of 105 CFU/ml for 5 days before a challenge with V. anguillarum at 104 to 105 CFU/ml for 1 h. Some fish were also exposed to P. fluorescens AH2 at 107 CFU/ml during the 1-h infection. The combined probiotic treatment resulted in a 46% reduction of calculated accumulated mortality; accumulated mortality was 25% after 7 days at 12°C in the probiotic-treated fish, whereas mortality was 47% in fish not treated with the probiont.

scholarly journals Large Phenotypic and Genetic Diversity of Prophages Induced from the Fish Pathogen Vibrio anguillarum

Viruses ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 983 ◽  
Author(s):  
Castillo ◽  
Andersen ◽  
Kalatzis ◽  
Middelboe
Keyword(s):  
Vibrio Anguillarum ◽  
Fish Pathogen ◽  
Pcr Assay ◽  
Phenotypic Properties ◽  
Specific Element ◽  
Group Iii ◽  
Multiplex Pcr Assay ◽  
Prophage Sequence ◽  
Fish And Shellfish ◽  
Efficient Transfer

Vibrio anguillarum is a marine pathogenic bacterium that causes vibriosis in fish and shellfish. Although prophage-like sequences have been predicted in V. anguillarum strains, many are not characterized, and it is not known if they retain the functional capacity to form infectious particles that can infect and lysogenize other bacterial hosts. In this study, the genome sequences of 28 V. anguillarum strains revealed 55 different prophage-related elements. Chemical and spontaneous induction allowed a collection of 42 phage isolates, which were classified in seven different groups according to a multiplex PCR assay. One shared prophage sequence, p41 (group III), was present in 17 V. anguillarum strains, suggesting that this specific element is very dynamically exchanged among V. anguillarum populations. Interestingly, the host range of genetically identical phages was highly dependent on the strains used for proliferation, indicating that phenotypic properties of phages were partly regulated by the host. Finally, experimental evidence displayed that the induced phage ɸVa_90-11-287_p41 was able to lysogenize V. anguillarum strain Ba35, and subsequently spontaneously become released from the lysogenized cells, demonstrating an efficient transfer of the phage among V. anguillarum strains. Altogether, the results showed large genetic and functional diversity and broad distribution of prophages in V. anguillarum, and demonstrated the potential of prophages as drivers of evolution in V. anguillarum strains.

scholarly journals Stumbling across the Same Phage: Comparative Genomics of Widespread Temperate Phages Infecting the Fish Pathogen Vibrio anguillarum

Viruses ◽  
2017 ◽  
Vol 9 (5) ◽  
pp. 122 ◽  
Author(s):  
Panos Kalatzis ◽  
Nanna Rørbo ◽  
Daniel Castillo ◽  
Jesper Mauritzen ◽  
Jóhanna Jørgensen ◽  
...  
Keyword(s):  
Comparative Genomics ◽  
Vibrio Anguillarum ◽  
Fish Pathogen

scholarly journals Molecular characterization of the TonB2 protein from the fish pathogen Vibrio anguillarum

2009 ◽  
Vol 418 (1) ◽  
pp. 49-59 ◽  
Author(s):  
Claudia S. López ◽  
R. Sean Peacock ◽  
Jorge H. Crosa ◽  
Hans J. Vogel
Keyword(s):  
Amino Acids ◽  
Solution Structure ◽  
Bacterial Species ◽  
Vibrio Anguillarum ◽  
Fish Pathogen ◽  
E Coli ◽  
Terminal Domain ◽  
C Terminus

In the fish pathogen Vibrio anguillarum the TonB2 protein is essential for the uptake of the indigenous siderophore anguibactin. Here we describe deletion mutants and alanine replacements affecting the final six amino acids of TonB2. Deletions of more than two amino acids of the TonB2 C-terminus abolished ferric-anguibactin transport, whereas replacement of the last three residues resulted in a protein with wild-type transport properties. We have solved the high-resolution solution structure of the TonB2 C-terminal domain by NMR spectroscopy. The core of this domain (residues 121–206) has an αββαβ structure, whereas residues 76–120 are flexible and extended. This overall folding topology is similar to the Escherichia coli TonB C-terminal domain, albeit with two differences: the β4 strand found at the C-terminus of TonB is absent in TonB2, and loop 3 is extended by 9 Å (0.9 nm) in TonB2. By examining several mutants, we determined that a complete loop 3 is not essential for TonB2 activity. Our results indicate that the β4 strand of E. coli TonB is not required for activity of the TonB system across Gram-negative bacterial species. We have also determined, through NMR chemical-shift-perturbation experiments, that the E. coli TonB binds in vitro to the TonB box from the TonB2-dependent outer membrane transporter FatA; moreover, it can substitute in vivo for TonB2 during ferric-anguibactin transport in V. anguillarum. Unexpectedly, TonB2 did not bind in vitro to the FatA TonB-box region, suggesting that additional factors may be required to promote this interaction. Overall our results indicate that TonB2 is a representative of a different class of TonB proteins.

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