Induction of TNF-.ALPHA. Production from Human Peripheral Blood Monocytes with .BETA.-1,3-Glucan Oligomer Prepared from Laminarin with .BETA.-1,3-Glucanase from Bacillus clausii NM-1.

2003 ◽  
Vol 95 (2) ◽  
pp. 192-195 ◽  
Author(s):  
NOBUMITSU MIYANISHI ◽  
YOSHIKO IWAMOTO ◽  
ETSUO WATANABE ◽  
TATSUYA ODA
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Tnf Alpha ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Bacillus Clausii ◽  
Alpha Production

TNF-alpha Production by Peripheral Blood Monocytes in Multiple Sclerosis Patients and Healthy Controls

2015 ◽  
Vol 44 (6) ◽  
pp. 590-601 ◽  
Author(s):  
Mehrdad Farrokhi ◽  
Masoud Etemadifar ◽  
Maryam Sadat Jafary Alavi ◽  
Sayyed Hamid Zarkesh-Esfahani ◽  
Mohaddeseh Behjati ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Peripheral Blood ◽  
Tnf Alpha ◽  
Healthy Controls ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Alpha Production ◽  
Multiple Sclerosis Patients

scholarly journals Interleukin 7 induces cytokine secretion and tumoricidal activity by human peripheral blood monocytes.

1991 ◽  
Vol 173 (4) ◽  
pp. 923-930 ◽  
Author(s):  
M R Alderson ◽  
T W Tough ◽  
S F Ziegler ◽  
K H Grabstein
Keyword(s):  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Optimal Concentration ◽  
Cytokine Secretion ◽  
Tnf Alpha ◽  
Northern Hybridization ◽  
Blood Monocytes ◽  
Tumoricidal Activity ◽  
Peripheral Blood Monocytes ◽  
Interleukin 7

Peripheral blood monocytes can be induced by stimuli such as bacterial lipopolysaccharide (LPS) to secrete an array of cytokines. We have studied the effects of interleukin 7 (IL-7) on human peripheral blood mononuclear cells (PBMC) and found that IL-7 is a relatively potent inducer of IL-6 secretion IL-6 protein levels were determined either by the B9 hybridoma growth factor assay or by enzyme-linked immunosorbent assay, and mRNA for IL-6 was analyzed by Northern hybridization. Detailed examination revealed that, among PBMC, monocytes, rather than lymphocytes, were secreting IL-6 in response to IL-7. In contrast to the low concentrations of IL-7 required to stimulate T cell growth and differentiation (as low as 0.1 ng/ml), relatively high concentrations of IL-7 were necessary to induce IL-6 secretion by monocytes (at least 10 ng/ml). An optimal concentration of IL-7 (100 ng/ml) induced monocytes to secrete 10-fold more IL-6 than an optimal concentration of IL-1 beta (10 ng/ml), and almost as much as LPS. However, significantly more IL-7 than IL-1 beta was required to induce detectable levels of IL-6. The kinetics of IL-6 secretion by monocytes were identical in response to IL-7, IL-1 beta, or LPS, with IL-6 protein detectable in culture supernatants as early as 2 h after the initiation of culture. IL-4 was found to markedly inhibit the ability of IL-7 or LPS to induce IL-6 mRNA and IL-6 secretion. In addition to promoting IL-6 production, IL-7 induced the secretion of immunoreactive IL-1 alpha, IL-1 beta, and tumor necrosis factor alpha (TNF-alpha) by monocytes. IL-7 also induced monocyte/macrophage tumoricidal activity against a human melanoma cell target, an activity that may be related to the secretion of IL-1 alpha, IL-1 beta, and TNF-alpha. Finally, we used a whole blood culture system as a bridge to in vivo analysis to demonstrate that IL-7 induces cytokine secretion in the absence of culture medium, fetal calf serum, and adherence to plastic. Our data suggest that IL-7, in addition to regulating lymphocyte growth and differentiation, has potent effects on cells of the monocytic lineage. Thus, IL-7 may be an important mediator in inflammation and in the macrophage immune response to tumors.

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