Application of the PHO5-Gene-Fusion Technology to Molecular Genetics and Biotechnology in Yeast.

2001 ◽  
Vol 91 (4) ◽  
pp. 325-338 ◽  
Author(s):  
SATOSHI HARASHIMA ◽  
YOSHINOBU KANEKO
Keyword(s):  
Molecular Genetics ◽  
Gene Fusion ◽  
Fusion Technology ◽  
Pho5 Gene

scholarly journals A genomic approach to gene fusion technology

2001 ◽  
Vol 98 (5) ◽  
pp. 2555-2560 ◽  
Author(s):  
T. K. Van Dyk ◽  
Y. Wei ◽  
M. K. Hanafey ◽  
M. Dolan ◽  
M. J. G. Reeve ◽  
...  
Keyword(s):  
Gene Fusion ◽  
Fusion Technology ◽  
Genomic Approach

Insertional gene fusion technology

FEBS Letters ◽  
1999 ◽  
Vol 457 (1) ◽  
pp. 1-4 ◽  
Author(s):  
Nobuhide Doi ◽  
Hiroshi Yanagawa
Keyword(s):  
Gene Fusion ◽  
Fusion Technology

scholarly journals Combination ofERG9Repression and Enzyme Fusion Technology for Improved Production of Amorphadiene inSaccharomyces cerevisiae

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Rama Raju Baadhe ◽  
Naveen Kumar Mekala ◽  
Sreenivasa Rao Parcha ◽  
Yalavarthy Prameela Devi
Keyword(s):  
Gene Fusion ◽  
Mevalonate Pathway ◽  
Artemisia Annua ◽  
Combinatorial Biosynthesis ◽  
Plant Origin ◽  
Fusion Technology ◽  
Precursor Molecule ◽  
Yeast Strains ◽  
Intermediate Metabolite ◽  
Enzyme Fusion

The yeast strain (Saccharomyces cerevisiae) MTCC 3157 was selected for combinatorial biosynthesis of plant sesquiterpene amorpha-4,11-diene. Our main objective was to overproduce amorpha 4-11-diene, which is a key precursor molecule of artemisinin (antimalarial drug) produced naturally in plantArtemisia annuathrough mevalonate pathway. Farnesyl diphosphate (FPP) is a common intermediate metabolite of a variety of compounds in the mevalonate pathway of yeast and leads to the production of ergosterols, dolichol and ubiquinone, and so forth. In our studies, FPP converted to amorphadiene (AD) by expressing heterologous amorphadiene synthase (ADS) in yeast. First,ERG9(squalane synthase) promoter of yeast was replaced with repressible methionine (MET3) promoter by using bipartite gene fusion method. Further to overcome the loss of the intermediate FPP through competitive pathways in yeast, fusion protein technology was adopted and farnesyldiphosphate synthase (FPPS) of yeast has been coupled with amorphadiene synthase (ADS) of plant origin (Artemisia annuaL.) where amorphadiene production was improved by 2-fold (11.2 mg/L) and 4-fold (25.02 mg/L) in yeast strains YCF-002 and YCF-005 compared with control strain YCF-AD (5.5 mg/L), respectively.

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

Molecular Genetics Of Acoustic Tumors

1992 ◽  
Vol 25 (5) ◽  
pp. 995-1009
Author(s):  
Michael J. Lanser
Keyword(s):  
Molecular Genetics
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