Characterization of a Flavinogenic Mutant of Methanol Yeast Cahdida boidinii and Its Extracellular Secretion of Riboflavin.

2000 ◽  
Vol 90 (1) ◽  
pp. 52-56 ◽  
Author(s):  
HERMAN SURYADI ◽  
NOBUYUKI YOSHIDA ◽  
KEIKO YAMADA-ONODERA ◽  
TOHORU KATSURAGI ◽  
YOSHIKI TANI
2000 ◽  
Vol 90 (1) ◽  
pp. 52-56 ◽  
Author(s):  
Herman Suryadi ◽  
Nobuyuki Yoshida ◽  
Keiko Yamada-Onodera ◽  
Tohoru Katsuragi ◽  
Yoshiki Tani

Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


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