Decolorization of Heat-Treatment Liquor of Waste Sludge by a Bioreactor Using Polyurethane Foam-Immobilized White Rot Fungus Equipped with an Ultramembrane Filtration Unit.

2000 ◽  
Vol 90 (4) ◽  
pp. 387-394
Author(s):  
MASANORI FUJITA ◽  
AKIRA ERA ◽  
MICHIHIKO IKE ◽  
SATOSHI SODA ◽  
NAOYUKI MIYATA ◽  
...  
1997 ◽  
Vol 33 (1) ◽  
pp. 35-45 ◽  
Author(s):  
NAOYUKI MIYATA ◽  
MASAKO YAMASHITA ◽  
MICHIHIKO IKE ◽  
KEISUKE IWAHORI ◽  
MASANORI FUJITA

2006 ◽  
Vol 1 (3) ◽  
Author(s):  
Dawen Gao ◽  
Xianghua Wen ◽  
Yonggang Zeng ◽  
Yi Qian

Most studies on decolourization of reactive dye with Phanerochaete chrysosporium have been performed under sterile conditions. In this paper, Phanerochaete chrysosporium, suspended in liquid medium, immobilized on nylon nets, and immobilized on polyurethane foams were studied to decolorize a textile-reactive dye in agitated liquid incubation under non-sterile conditions. The results showed that the decolourization against reactive brilliant red K-2BP by mycelia pellets in suspended cultures, the fungi immobilized on nylon nets and immobilized on polyurethane foams were 0%, 52% and 95%, respectively. The suspended incubation system and the system with nylon nets were contaminated with yeasts, which deteriorated the decolourization effects. The three-dimensional reticular structure of polyurethane foam was benefit to Phanerochaete chrysosporium growth in spreading mycelia taking nutrients and oxygen effectively. The foam could quickly absorb liquid medium into its holes, leaving much less nutrients to support the growth of single cell micro-organisms such as yeast in liquid. The micro-environment existed in polyurethane foam was close to the wild growth conditions of white rot fungus in nature. In conclusion, immobilized culture with polyurethane foam carriers is advantaged to reduce the colonization of other micro-organisms, and makes Phanerochaete chrysosporium possess higher decolorizing ability under non-sterile conditions.


2020 ◽  
Vol 19 (2) ◽  
pp. 112-116
Author(s):  
Luana Maria dos Santos ◽  
Erick Martins Nieri ◽  
Flávia Alves Pereira ◽  
Sandra Mara Krefta ◽  
Jéssica Batista da Mata

The companies in the forestry sector seek highly durable wood, prioritizing the use of planted forests, and the thermal treatment is a viable alternative to provide quality to these woods. Thus, the objective of the study was to evaluate the resistance of the heat-treated Pinus taeda L wood by the attack of white rot fungus Trametes versicolor (L.) CG Lloyd. The P. taeda L. wood was deployed in joinery to obtain samples with dimensions of 30 x 15 x 2.5 cm (length x width x thickness), being treated at temperatures of 140, 160 and 180ºC. From these, 1.0 x 1.0 x 1.0 cm specimens were obtained and subjected to the fungus for six weeks. The accelerated rot test was carried out in accordance with the ASTM D2017 standard. The experiment was conducted in a completely randomized design with four treatments (T1 = 0; T2 = 140°C; T3 = 160°C and T4 = 180°C) and nine replications. The loss of mass caused by the fungus was less in heat treated wood. On the other hand, the increase in temperature did not provide greater resistance to the material. In conclusion, heat treatment has reduced the degradation caused by the white rot fungus Trametes versicolor. Heat treatment at 140°C is indicated to avoid loss of mass in P. taeda L. woods submitted to the fungus T. versicolor. All treated woods were classified as highly resistant.


2021 ◽  
Vol 891 (1) ◽  
pp. 012010
Author(s):  
N Istriana ◽  
T Priadi

Abstract Manii wood (Maesopsiss eminii Engl.) is a fast grown species that has low durability (class IV) and requires preservation. Boric acid is generally used in wood preservation. However, its application needs to be combined with other treatments to prevent from leaching. This study aimed to evaluate the resistance of manii wood after double impregnation of boric acid and chitosan or glycerol and heat treatment. Heating temperatures were 70ºC and 140ºC. The durability test was carried out against white rot fungus (Schizophyllum commune), dry wood termites (Cryptotermes cynocephalus), subterranean termites (Coptotermes curvignathus) according to SNI 7207-2014 standard, and field test based on ASTM D 1758-08 standard. The retention of boric acid in manii wood was 15,2 ± 1,0 kg ma−3. The interaction of boric acid and chitosan/glycerol impregnations and heat treatment significantly affected the wood resistance against decay fungi and termites. Impregnation of boric acid and glycerol by heating at 140ºC increased the resistance of wood against dry wood and subterranean termites. Double impregnation of boric acid and chitosan followed by heating at 140ºC was the best treatment in this research that improved significantly the resistance of manii wood against white rot fungi, dry wood termites and subterranean termites.


2012 ◽  
Vol 3 (1) ◽  
pp. 20-21
Author(s):  
A.Sangeetha A.Sangeetha ◽  
◽  
K.Thanigai K.Thanigai ◽  
Narasimhamurthy Narasimhamurthy ◽  
S.K.Nath S.K.Nath

2020 ◽  
Vol 93 (9) ◽  
pp. 289-292
Author(s):  
Yumi SHIMIZU ◽  
Shuma SATHO ◽  
Taro NAKAJIMA ◽  
Hiroaki KOUZAI ◽  
Kiminori SHIMIZU

2018 ◽  
Vol 69 (1) ◽  
pp. 38-44
Author(s):  
Nicoleta Mirela Marin ◽  
Olga Tiron ◽  
Luoana Florentina Pascu ◽  
Mihaela Costache ◽  
Mihai Nita Lazar ◽  
...  

This study investigates the synergistic effects of ion exchange and biodegradation methods to remove the Acid Blue 193 also called Gryfalan Navy Blue RL (GNB) dye from wastewater. Ion exchange studies were performed using a strongly basic anion exchange resin Amberlite IRA 400. The equilibrium was characterized by a kinetic and thermodynamic points of view, establishing that the sorption of the GNB dye was subject to the Freundlich isotherm model with R2 = 0.8710. Experimental results showed that the activated resin can removed up to 93.4% when the concentration of dye solution is 5.62�10-2 mM. The biodegradation of the GNB was induced by laccase, an enzyme isolated from white-rot fungus. It was also analyzed the role of pH and dye concentration on GNB biodegradation, so 5�10-2 mM dye had a maximum discoloration efficiency of 82.9% at pH of 4. The laccase showed a very fast and robust activity reaching in a few minutes a Km value of 2.2�10-1mM. In addition, increasing the GNB concentration up to 8�10-1 mM did not triggered a substrat inhibition effect on the laccase activity. Overall, in this study we proposed a mixt physicochemical and biological approach to enhance the GNB removal and biodegradability from the wastewaters and subsequently the environment.


1991 ◽  
Vol 24 (3-4) ◽  
pp. 189-198 ◽  
Author(s):  
V. P. Lankinen ◽  
M. M. Inkeröinen ◽  
J. Pellinen ◽  
A. I. Hatakka

Decrease of adsorbable organic chlorine (AOX) is becoming the most important criterion for the efficiency of pulp mill effluent treatment in the 1990s. Two methods, designated MYCOR and MYCOPOR which utilize the white-rot fungus Phanerochaete chrysosporium have earlier been developed for the color removal of pulp mill effluents, but the processes have also a capacity to decrease the amount of chlorinated organic compounds. Lignin peroxidases (ligninases) produced by P. chrvsosporium may dechlorinate chlorinated phenols. In this work possibilities to use selected white-rot fungi in the treatment of E1-stage bleach plant effluent were studied. Phlebia radiata. Phanerochaete chrvsosporium and Merulius (Phlebia) tremellosus were compared in shake flasks for their ability to produce laccase, lignin peroxidase(s) and manganese-dependent peroxidase(s) and to remove color from a medium containing effluent. Softwood bleaching effluents were treated by carrier-immobilized P. radiata in 2 1 bioreactors and a 10 1 BiostatR -fermentor. Dechlorination was followed using Cl ion and AOX determinations. All fungi removed the color of the effluent. In P. radiata cultivations AOX decrease was ca. 4 mg l−1 in one day. Apparent lignin peroxidase activities as determined by veratryl alcohol oxidation method were negligible or zero in a medium with AOX content of ca. 60 mg l−1, prepared using about 20 % (v/v) of softwood effluent. However, the purification of extracellular enzymes implied that large amounts of lignin peroxidases were present in the medium and, after the purification, in active form. Enzyme proteins were separated using anion exchange chromatography, and they were further characterized by electrophoresis (SDS-PAGE) to reveal the kind of enzymes that were present during AOX decrease and color removal. The most characteristic lignin peroxidase isoenzymes in effluent media were LiP2 and LiP3.


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